Comparison of enterotoxigenicEscherichia coliisolated from surface water and diarrhoeal stool samples in Bangladesh

2007 ◽  
Vol 53 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Y A Begum ◽  
K A Talukder ◽  
G B Nair ◽  
S I Khan ◽  
A-M Svennerholm ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Surface Water ◽  
Water Samples ◽  
Gel Electrophoresis ◽  
Water Sources ◽  
Pulsed Field Gel Electrophoresis ◽  
Enterotoxigenic Escherichia Coli ◽  
Watery Diarrhea ◽  
Pulsed Field ◽  
Surface Water Samples

Enterotoxigenic Escherichia coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children. Although the prevalence of ETEC is high in Bangladesh and infections can be spread through food and contaminated water, limited information is available about ETEC in the surface water. We carried out studies to isolate ETEC from surface water samples from ponds, rivers, and a lake from a site close to field areas known to have a high incidence of diarrhea in Dhaka, Bangladesh, and Matlab, Bangladesh. ETEC strains isolated from the water sources were compared with ETEC strains isolated from patients with diarrhea at two hospitals in these areas. ETEC were isolated from 30% (45 of 150) of the samples from the surface water sources and 19% (518 of 2700) of the clinical specimens. One hundred ETEC strains isolated from patients with similar phenotypes as the environmental strains were compared for phenotypic and genotypic properties. The most common O serogroups on ETEC were O6, O25, O78, O115, and O126 in both types of strains. Pulsed-field gel electrophoresis analyses of the ETEC strains showed that multiple clones of ETEC were present within each colonization factor type and that some clones detected in the environment were also isolated from the stools of patients. The strains showed multiple and similar antibiotic resistance patterns. This study shows that ETEC is prevalent in surface water sources in Bangladesh suggesting a possible reason for the endemicity of this pathogen in Bangladesh.Key words: enterotoxigenic Escherichia coli (ETEC), surface water samples, colonization factors, pulsed-field gel electrophoresis, toxin types.

Concurrent Outbreaks of Shigella sonnei and Enterotoxigenic Escherichia coli Infections Associated with Parsley: Implications for Surveillance and Control of Foodborne Illness†

2003 ◽  
Vol 66 (4) ◽  
pp. 535-541 ◽  
Author(s):  
TIMOTHY S. NAIMI ◽  
JULIE H. WICKLUND ◽  
SONJA J. OLSEN ◽  
GERARD KRAUSE ◽  
JOY G. WELLS ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Distribution Networks ◽  
Pulsed Field Gel Electrophoresis ◽  
Shigella Sonnei ◽  
Enterotoxigenic Escherichia Coli ◽  
Health Concern ◽  
Foodborne Disease ◽  
Pulsed Field

In recent years, the globalization of the food supply and the development of extensive food distribution networks have increased the risk of foodborne disease outbreaks involving multiple states or countries. In particular, outbreaks associated with fresh produce have emerged as an important public health concern. During July and August 1998, eight restaurant-associated outbreaks of shigellosis caused by a common strain of Shigella sonnei occurred in the United States and Canada. The outbreak strain was characterized by unique pulsed-field gel electrophoresis patterns. Epidemiologic investigation determined that the illness was associated with the ingestion of parsley at four restaurants; at the other four restaurants, the majority of the people who contracted the illness ate parsley. Isolates from patrons in two unrelated restaurant-associated enterotoxigenic Escherichia coli (ETEC) outbreaks in Minnesota shared a common serotype and pulsed-field gel electrophoresis (PFGE) pattern. Parsley was the implicated or suspected source of both ETEC outbreaks. In each of the outbreak-associated restaurants, parsley was chopped, held at room temperature, and used as an ingredient or garnish for multiple dishes. Infected food workers at several restaurants may also have contributed to the propagation of the outbreak. The sources of parsley served in outbreak-associated restaurants were traced, and a 1,600-acre farm in Baja California, Mexico, was identified as a likely source of the parsley implicated in six of the seven Shigella outbreaks and as a possible source of the parsley implicated in the two ETEC outbreaks. Global food supplies and large distribution networks demand strengthened laboratory and epidemiologic capacity to enable state and local public health agencies to conduct foodborne disease surveillance and to promote effective responses to multistate outbreaks.

scholarly journals Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

2009 ◽  
Vol 21 (4) ◽  
pp. 510-516 ◽  
Author(s):  
Su In Lee ◽  
Nabin Rayamahji ◽  
Won Jung Lee ◽  
Seung Bin Cha ◽  
Min Kyung Shin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

scholarly journals Longitudinal Study of Shiga Toxin-Producing Escherichia coli Shedding in Sheep Feces: Persistence of Specific Clones in Sheep Flocks

2009 ◽  
Vol 75 (6) ◽  
pp. 1769-1773 ◽  
Author(s):  
Sergio Sánchez ◽  
Remigio Martínez ◽  
Alfredo García ◽  
Jorge Blanco ◽  
Jesús E. Blanco ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Longitudinal Study ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Individual Animal ◽  
Pulsed Field ◽  
The Individual ◽  
Sheep Feces

ABSTRACT To provide information on the persistence and maintenance of colonization with Shiga toxin-producing Escherichia coli (STEC) in sheep, pulsed-field gel electrophoresis analysis of STEC isolates (n = 145) belonging to serogroups O5, O91, and O146 from 39 healthy animals was performed in a 12-month longitudinal study carried out with four sheep flocks. At the flock level as well as the individual-animal level, the same clones were obtained on sampling occasions separated by as much as 11 months.

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