scholarly journals A new multidrug‐resistant enterotoxigenic Escherichia coli pulsed‐field gel electrophoresis cluster associated with enrofloxacin non‐susceptibility in diseased pigs

2020 ◽  
Author(s):  
M. Lagarde ◽  
G. Vanier ◽  
G. Desmarais ◽  
H‐R. Kohan‐Ghadr ◽  
J. Arsenault ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Enterotoxigenic Escherichia Coli ◽  
Pulsed Field

Concurrent Outbreaks of Shigella sonnei and Enterotoxigenic Escherichia coli Infections Associated with Parsley: Implications for Surveillance and Control of Foodborne Illness†

2003 ◽  
Vol 66 (4) ◽  
pp. 535-541 ◽  
Author(s):  
TIMOTHY S. NAIMI ◽  
JULIE H. WICKLUND ◽  
SONJA J. OLSEN ◽  
GERARD KRAUSE ◽  
JOY G. WELLS ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Distribution Networks ◽  
Pulsed Field Gel Electrophoresis ◽  
Shigella Sonnei ◽  
Enterotoxigenic Escherichia Coli ◽  
Health Concern ◽  
Foodborne Disease ◽  
Pulsed Field

In recent years, the globalization of the food supply and the development of extensive food distribution networks have increased the risk of foodborne disease outbreaks involving multiple states or countries. In particular, outbreaks associated with fresh produce have emerged as an important public health concern. During July and August 1998, eight restaurant-associated outbreaks of shigellosis caused by a common strain of Shigella sonnei occurred in the United States and Canada. The outbreak strain was characterized by unique pulsed-field gel electrophoresis patterns. Epidemiologic investigation determined that the illness was associated with the ingestion of parsley at four restaurants; at the other four restaurants, the majority of the people who contracted the illness ate parsley. Isolates from patrons in two unrelated restaurant-associated enterotoxigenic Escherichia coli (ETEC) outbreaks in Minnesota shared a common serotype and pulsed-field gel electrophoresis (PFGE) pattern. Parsley was the implicated or suspected source of both ETEC outbreaks. In each of the outbreak-associated restaurants, parsley was chopped, held at room temperature, and used as an ingredient or garnish for multiple dishes. Infected food workers at several restaurants may also have contributed to the propagation of the outbreak. The sources of parsley served in outbreak-associated restaurants were traced, and a 1,600-acre farm in Baja California, Mexico, was identified as a likely source of the parsley implicated in six of the seven Shigella outbreaks and as a possible source of the parsley implicated in the two ETEC outbreaks. Global food supplies and large distribution networks demand strengthened laboratory and epidemiologic capacity to enable state and local public health agencies to conduct foodborne disease surveillance and to promote effective responses to multistate outbreaks.

scholarly journals Molecular Epidemiology of Ceftiofur-Resistant Escherichia coli Isolates from Dairy Calves

2006 ◽  
Vol 72 (6) ◽  
pp. 3940-3948 ◽  
Author(s):  
Sarah C. Donaldson ◽  
Beth A. Straley ◽  
Narasimha V. Hegde ◽  
Ashish A. Sawant ◽  
Chitrita DebRoy ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Dairy Herd ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Dairy Calves ◽  
Disk Diffusion ◽  
Pulsed Field ◽  
E Coli ◽  
Clonal Type

ABSTRACT Healthy calves (n = 96, 1 to 9 weeks old) from a dairy herd in central Pennsylvania were examined each month over a five-month period for fecal shedding of ceftiofur-resistant gram-negative bacteria. Ceftiofur-resistant Escherichia coli isolates (n = 122) were characterized by antimicrobial resistance (disk diffusion and MIC), serotype, pulsed-field gel electrophoresis subtypes, beta-lactamase genes, and virulence genes. Antibiotic disk diffusion assays showed that the isolates were resistant to ampicillin (100%), ceftiofur (100%), chloramphenicol (94%), florfenicol (93%), gentamicin (89%), spectinomycin (72%), tetracycline (98%), ticarcillin (99%), and ticarcillin-clavulanic acid (99%). All isolates were multidrug resistant and displayed elevated MICs. The E. coli isolates belonged to 42 serotypes, of which O8:H25 was the predominant serotype (49.2%). Pulsed-field gel electrophoresis classified the E. coli isolates into 27 profiles. Cluster analysis showed that 77 isolates (63.1%) belonged to one unique group. The prevalence of pathogenic E. coli was low (8%). A total of 117 ceftiofur-resistant E. coli isolates (96%) possessed the bla CMY2 gene. Based on phenotypic and genotypic characterization, the ceftiofur-resistant E. coli isolates belonged to 59 clonal types. There was no significant relationship between calf age and clonal type. The findings of this study revealed that healthy dairy calves were rapidly colonized by antibiotic-resistant strains of E. coli shortly after birth. The high prevalence of multidrug-resistant nonpathogenic E. coli in calves could be a significant source of resistance genes to other bacteria that share the same environment.

Comparison of enterotoxigenicEscherichia coliisolated from surface water and diarrhoeal stool samples in Bangladesh

2007 ◽  
Vol 53 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Y A Begum ◽  
K A Talukder ◽  
G B Nair ◽  
S I Khan ◽  
A-M Svennerholm ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Surface Water ◽  
Water Samples ◽  
Gel Electrophoresis ◽  
Water Sources ◽  
Pulsed Field Gel Electrophoresis ◽  
Enterotoxigenic Escherichia Coli ◽  
Watery Diarrhea ◽  
Pulsed Field ◽  
Surface Water Samples

Enterotoxigenic Escherichia coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children. Although the prevalence of ETEC is high in Bangladesh and infections can be spread through food and contaminated water, limited information is available about ETEC in the surface water. We carried out studies to isolate ETEC from surface water samples from ponds, rivers, and a lake from a site close to field areas known to have a high incidence of diarrhea in Dhaka, Bangladesh, and Matlab, Bangladesh. ETEC strains isolated from the water sources were compared with ETEC strains isolated from patients with diarrhea at two hospitals in these areas. ETEC were isolated from 30% (45 of 150) of the samples from the surface water sources and 19% (518 of 2700) of the clinical specimens. One hundred ETEC strains isolated from patients with similar phenotypes as the environmental strains were compared for phenotypic and genotypic properties. The most common O serogroups on ETEC were O6, O25, O78, O115, and O126 in both types of strains. Pulsed-field gel electrophoresis analyses of the ETEC strains showed that multiple clones of ETEC were present within each colonization factor type and that some clones detected in the environment were also isolated from the stools of patients. The strains showed multiple and similar antibiotic resistance patterns. This study shows that ETEC is prevalent in surface water sources in Bangladesh suggesting a possible reason for the endemicity of this pathogen in Bangladesh.Key words: enterotoxigenic Escherichia coli (ETEC), surface water samples, colonization factors, pulsed-field gel electrophoresis, toxin types.

scholarly journals Carbapenem-Resistant KPC-2-Producing Escherichia coli in a Tel Aviv Medical Center, 2005 to 2008

2010 ◽  
Vol 54 (6) ◽  
pp. 2687-2691 ◽  
Author(s):  
Moran G. Goren ◽  
Shiri Navon-Venezia ◽  
Inna Chmelnitsky ◽  
Yehuda Carmeli
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Medical Center ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Beta Lactamase ◽  
Pulsed Field ◽  
Extended Spectrum Beta Lactamase ◽  
Tel Aviv ◽  
Carbapenem Resistant

ABSTRACT All of the carbapenem-resistant Escherichia coli (CREC) isolates identified in our hospital from 2005 to 2008 (n = 10) were studied. CREC isolates were multidrug resistant, all carried bla KPC-2, and six of them were also extended-spectrum beta-lactamase producers. Pulsed-field gel electrophoresis indicated six genetic clones; within the same clone, similar transferable bla KPC-2-containing plasmids were found whereas plasmids differed between clones. Tn4401 elements were identified in all of these plasmids.

scholarly journals Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

2009 ◽  
Vol 21 (4) ◽  
pp. 510-516 ◽  
Author(s):  
Su In Lee ◽  
Nabin Rayamahji ◽  
Won Jung Lee ◽  
Seung Bin Cha ◽  
Min Kyung Shin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

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