Antigenic and genetic characterization of serogroup C meningococci isolated from invasive meningococcal disease cases in Canada from 1999 to 2003

2005 ◽  
Vol 51 (7) ◽  
pp. 523-530 ◽  
Author(s):  
Dennis K.S Law ◽  
Jan Stoltz ◽  
Averil M Henderson ◽  
Raymond S.W Tsang
Keyword(s):  
Membrane Protein ◽  
Key Words ◽  
Outer Membrane Protein ◽  
Meningococcal Disease ◽  
Clonal Complex ◽  
Genetic Characterization ◽  
Hot Spot ◽  
Invasive Meningococcal Disease ◽  
Vaccination Campaigns

Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal complex of electrophoretic type ET-15. However, after more than a decade of endemic disease as well as a number of outbreaks and many vaccination campaigns, both genetic and antigenic variants of the serogroup C serotype 2a meningococci were noted. Such variants include strains characterized as C:2a:P1.5 and C:2a:P1.7,1 as well as a non-serotypeable phenotype due to a mutational hot spot on the serotype 2a PorB outer-membrane protein. Meningococci characterized by the antigen formula B:2a:P1.5,2 and B:2a:P1.7,1 have also been found, which suggests capsule switching. Besides the clonal group of ET-15/ET-37, small numbers of serogroup C isolates were found to belong to the clonal complexes of ST-8 (Cluster A4), ST-41/44 (Lineage 3), ST-35, and ST-269.Key words: serogroup C, meningococci, genetic, antigenic, variants.

scholarly journals Characterization of Serogroup ANeisseria meningitidisfrom Invasive Meningococcal Disease Cases in Canada Between 1979 and 2006: Epidemiological Links to Returning Travellers

2008 ◽  
Vol 19 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Angela M Sloan ◽  
Averil M Henderson ◽  
Raymond SW Tsang
Keyword(s):  
Meningococcal Disease ◽  
Clonal Complex ◽  
Disease Incidence ◽  
Developing Nations ◽  
Invasive Meningococcal Disease ◽  
Protein Antigens ◽  
Genes Encoding ◽  
Returning Travellers ◽  
The 1960S

INTRODUCTION: Serogroup ANeisseria meningitidishas repeatedly caused epidemics of invasive meningococcal disease (IMD) in developing nations since the 1960s. The present study is the first detailed study of serogroup A bacteria isolated in Canada.METHODS: Thirty-four serogroup A meningococcal isolates collected from individuals with IMD in Canada between 1979 and 2006 were characterized by serology and multilocus sequence typing of seven housekeeping enzyme genes and genes encoding three outer membrane protein antigens.RESULTS: Isolates were assigned to either the sequence type (ST)-1 or the ST-5 clonal complex. Clones within the ST-1 complex were recovered between 1979 and 1992, while clones of the ST-5 complex were isolated between 1987 and 2006; respectively, they accounted for 70.6% and 29.4% of all isolates studied. Isolates of the ST-1 complex were characterized by serosubtype antigen P1.3 or P1.3,6 with PorB allele 60 (serotype 4) and FetA sequence F5-1, while isolates of the ST-5 complex were characterized by serosubtype antigen P1.9 with PorB allele 47 (also serotype 4) and FetA sequence F3-1.CONCLUSIONS: The Canadian serogroup A IMD isolates likely originated in travellers returning from hyperendemic or epidemic areas of the globe where serogroup A bacteria circulate. Although the Canadian cases of serogroup A IMD were caused by clones known to have caused epidemics in developing countries, disease incidence remained low in Canada.

scholarly journals Molecular Cloning and Characterization of theoprQGene Coding for Outer Membrane Protein OprE3 ofPseudomonas aeruginosa

1999 ◽  
Vol 43 (3) ◽  
pp. 297-301 ◽  
Author(s):  
Kiyomi Okamoto ◽  
Naomasa Gotoh ◽  
Hideto Tsujimoto ◽  
Hiroshi Yamada ◽  
Eisaku Yoshihara ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Molecular Cloning ◽  
Outer Membrane Protein
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