Confirmation of invasive meningococcal disease by single point estimation of IgM antibody to outer membrane protein of Neisseria meningitidis

Pathology ◽  
2001 ◽  
Vol 33 (3) ◽  
pp. 375-378 ◽  
Author(s):  
Peter W. Robertson ◽  
Porl Reinbott ◽  
Yvonne Duffy ◽  
Enzo Binotto ◽  
John W. Tapsall
Keyword(s):  
Membrane Protein ◽  
Neisseria Meningitidis ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Meningococcal Disease ◽  
Single Point ◽  
Point Estimation ◽  
Invasive Meningococcal Disease ◽  
Igm Antibody

scholarly journals Use of transformation to construct antigenic hybrids of the class 1 outer membrane protein in Neisseria meningitidis.

1993 ◽  
Vol 61 (10) ◽  
pp. 4217-4224 ◽  
Author(s):  
P van der Ley ◽  
J van der Biezen ◽  
P Hohenstein ◽  
C Peeters ◽  
J T Poolman
Keyword(s):  
Membrane Protein ◽  
Neisseria Meningitidis ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Class 1

scholarly journals Protection of Mice against Brucellosis by Intranasal Immunization with Brucella melitensis Lipopolysaccharide as a Noncovalent Complex with Neisseria meningitidis Group B Outer Membrane Protein

2002 ◽  
Vol 70 (7) ◽  
pp. 3324-3329 ◽  
Author(s):  
Apurba K. Bhattacharjee ◽  
Lillian Van De Verg ◽  
Mina J. Izadjoo ◽  
Liang Yuan ◽  
Ted L. Hadfield ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Antibody Response ◽  
Neisseria Meningitidis ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Brucella Melitensis ◽  
Noncovalent Complex ◽  
High Titer ◽  
Intranasal Immunization ◽  
Group B

ABSTRACT Intranasal immunization of mice with purified Brucella melitensis lipopolysaccharide (LPS) as a noncovalent complex with Neisseria meningitidis group B outer membrane protein (GBOMP) elicited a high-titer anti-LPS systemic antibody response and a significant mucosal antibody response. The anti-LPS immunoglobulin G (IgG) antibody was predominantly of the IgG1 subtype, although there was some response of the IgG2a, IgG2b, and IgG3 subtypes. The antibody titer remained high for 16 weeks postimmunization. Immunized mice and sham-immunized control mice were challenged intranasally with 104 CFU of virulent B. melitensis strain 16 M 4 weeks after the second dose of vaccine. The numbers of bacteria in lungs, livers, and spleens at 3 days, 9 days, and 8 weeks postchallenge were determined. Bacteria were found in lungs of all mice on day 3, but there was no disseminated infection of liver or spleen. By day 9, 40% of the mice had infected spleens and livers. At 8 weeks postchallenge, spleens of 25 of 62 immunized mice were infected, compared to 61 of 62 control mice (P < 0.0001). The livers of 12 of 43 immunized mice were infected, compared to 22 of 36 control mice (P = 0.005). In contrast, the lungs of 26 of 46 immunized mice were still infected, compared to 27 of 44 control mice. The numbers of bacterial CFU in lungs of immunized and control animals were identical. These studies show that intranasal immunization with B. melitensis LPS-GBOMP subunit vaccine significantly protects mice against intranasal challenge with virulent B. melitensis. Vaccination reduces bacterial dissemination to spleen and liver but has no effect on the course of lung infection.

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