Comparison of methods for the concentration ofCryptosporidiumoocysts andGiardiacysts from raw waters

2004 ◽  
Vol 50 (9) ◽  
pp. 675-682 ◽  
Author(s):  
Christobel Ferguson ◽  
Christine Kaucner ◽  
Martin Krogh ◽  
Daniel Deere ◽  
Malcolm Warnecke
Keyword(s):  
Internal Control ◽  
Membrane Filtration ◽  
Immunomagnetic Separation ◽  
The Other ◽  
Recovery Efficiency ◽  
Comparison Of Methods ◽  
Raw Water ◽  
High Recovery ◽  
Cryptosporidium Oocysts ◽  
Giardia Cysts

This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration–elution methods: flatbed membranes, FiltaMaxTMfoam, EnvirochekTMHV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining steps was also determined. Analysis of variance of arcsine-transformed data demonstrated that recovery of Cryptosporidium oocysts by 2 of the methods was statistically equivalent (flatbed filtration 26.7% and Hemoflow 28.3%), with FiltaMaxTMand EnvirochekTMHV recoveries significantly lower (18.9% and 18.4%). Recovery of Giardia cysts was significantly higher using flatbed membrane filtration (42.2%) compared with the other 3 methods (EnvirochekTMHV 29.3%, FiltaMaxTM29.0%, and Hemoflow 20.9%). All methods were generally acceptable and are suitable for laboratory use; 2 of the methods are also suitable for field use (FiltaMaxTMand EnvirochekTMHV). In conclusion, with recoveries generally being statistically equivalent or similar, practical considerations become important in determining which filters to use for particular circumstances. The results indicate that while low-turbidity or "finished" waters can be processed with consistently high recovery efficiencies, recoveries from raw water samples differ significantly with variations in raw water quality. The use of an internal control with each raw water sample is therefore highly recommended.Key words: catchments, EnvirochekTMHV, Hemoflow, FiltaMaxTM, flatbed filtration.

scholarly journals Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater

2015 ◽  
Vol 13 (3) ◽  
pp. 811-818 ◽  
Author(s):  
Raphael Corrêa Medeiros ◽  
Luiz Antonio Daniel
Keyword(s):  
Membrane Filtration ◽  
Low Cost ◽  
Immunomagnetic Separation ◽  
Species Differentiation ◽  
Treated Effluent ◽  
Cryptosporidium Oocysts ◽  
Advance Research ◽  
Wastewater Samples ◽  
Raw Wastewater ◽  
Giardia Cysts

More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo)cysts from wastewater samples using ColorSeed®. Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries.

Determination of the Recovery Efficiency of Cryptosporidium Oocysts and Giardia Cysts from Seeded Bivalve Mollusks

2013 ◽  
Vol 76 (1) ◽  
pp. 93-98 ◽  
Author(s):  
FRANCISKA M. SCHETS ◽  
HAROLD H. J. L. van den BERG ◽  
ANA MARIA de RODA HUSMAN
Keyword(s):  
Quantitative Assessment ◽  
Intestinal Parasites ◽  
Cryptosporidium Oocyst ◽  
Immunomagnetic Separation ◽  
Giardia Cyst ◽  
Bivalve Mollusks ◽  
Recovery Efficiency ◽  
Risk Of Infection ◽  
Cryptosporidium Oocysts

The intestinal parasites Cryptosporidium and Giardia are transmitted by water and food and cause human gastroenteritis. Filter-feeding bivalve mollusks, such as oysters and mussels, filter large volumes of water and thus concentrate such pathogens, which makes these bivalves potential vectors of disease. To assess the risk of infection from consumption of contaminated bivalves, parasite numbers and parasite recovery data are required. A modified immunomagnetic separation (IMS) procedure was used to determine Cryptosporidium oocyst and Giardia cyst numbers in individually homogenized oysters (Crassostrea gigas) and mussels (Mytilus edulis). About 12% of the commercial bivalves were positive, with low (oo)cyst numbers per specimen. The recovery efficiency of the IMS procedure was systematically evaluated. Experiments included seeding of homogenized bivalves and whole animals with 100 to 1,000 (oo)cysts. Both seeding procedures yielded highly variable recovery rates. Median Cryptosporidium recoveries were 7.9 to 21% in oysters and 62% in mussels. Median Giardia recoveries were 10 to 25% in oysters and 110% in mussels. Giardia recovery was significantly higher than Cryptosporidium recovery. (Oo)cysts were less efficiently recovered from seeded whole animals than from seeded homogenates, with median Cryptosporidium recoveries of 5.3% in oysters and 45% in mussels and median Giardia recoveries of 4.0% in oysters and 82% in mussels. Both bivalve homogenate seeding and whole animal seeding yielded higher (oo)cyst recovery in mussels than in oysters, likely because of the presence of less shellfish tissue in IMS when analyzing the smaller mussels compared with the larger oysters, resulting in more efficient (oo)cyst extraction. The data generated in this study may be used in the quantitative assessment of the risk of infection with Cryptosporidium or Giardia associated with the consumption of raw bivalve mollusks. This information may be used for making risk management decisions.

Occurrence of Cryptosporidium oocysts and Giardia cysts in a conventional water purification plant

2001 ◽  
Vol 43 (12) ◽  
pp. 89-92 ◽  
Author(s):  
A. Hashimoto ◽  
T. Hirata ◽  
S. Kunikane
Keyword(s):  
Water Purification ◽  
Water Samples ◽  
Geometric Mean ◽  
Giardia Cyst ◽  
Raw Water ◽  
Observation Level ◽  
Cryptosporidium Oocysts ◽  
Purification Plant ◽  
Mean Concentration ◽  
Giardia Cysts

A one-year monitoring of Cryptosporidium oocysts and Giardia cysts was conducted at a water purification plant. A total of thirteen 50 L samples of river source water and twenty-six 2,000 L samples of filtered water (treated by coagulation-flocculation, sedimentation and rapid filtration) were concentrated using a hollow fibre ultrafiltration membrane module at a purification plant. Cryptosporidium oocysts were detected in all raw water samples with a geometric mean concentration of 400 oocysts/m3 (range 160-1,500 oocysts/m3). Giardia cysts were detected in 12/13 raw waters (92%) with a geometric mean concentration of 170 cysts/m3 (range 40-580 oocysts/m3). Probability distributions of both Cryptosporidium oocyst and Giardia cyst concentration in raw water were nearly log-normal. In filtered water samples, Cryptosporidium oocysts were detected in 9/26 samples (35%) with a geometric mean concentration of 1.2 oocysts/m3 (range 0.5-8 oocysts/m3) and Giardia cysts in three samples (12%) with 0.8 cysts/m3 (range 0.5-2 oocysts/m3). The estimated removal of Cryptosporidium oocysts and Giardia cysts was, respectively, 2.54 log10 and 2.53 log10 on the basis of geometric means, 3.20 and 3.57 log10 on the basis of 50% observation level and 2.70 and 2.90 log10 on the basis of 90% observation level.

scholarly journals Development of a Method for Detection of Giardia duodenalis Cysts on Lettuce and for Simultaneous Analysis of Salad Products for the Presence of Giardia Cysts and Cryptosporidium Oocysts

2007 ◽  
Vol 73 (22) ◽  
pp. 7388-7391 ◽  
Author(s):  
N. Cook ◽  
R. A. B. Nichols ◽  
N. Wilkinson ◽  
C. A. Paton ◽  
K. Barker ◽  
...  
Keyword(s):  
Internal Control ◽  
Giardia Duodenalis ◽  
Immunomagnetic Separation ◽  
Internal Controls ◽  
Food Sample ◽  
Simultaneous Analysis ◽  
Natural Food ◽  
Cryptosporidium Oocysts ◽  
Natural Foods ◽  
Texas Red

ABSTRACT We report a method for detecting Giardia duodenalis cysts on lettuce, which we subsequently use to examine salad products for the presence of Giardia cysts and Cryptosporidium oocysts. The method is based on four basic steps: extraction of cysts from the foodstuffs, concentration of the extract and separation of the cysts from food materials, staining of the cysts to allow their visualization, and identification of cysts by microscopy. The concentration and separation steps are performed by centrifugation, followed by immunomagnetic separation using proprietary kits. Cyst staining is also performed using proprietary reagents. The method recovered 46.0% ± 19.0% (n = 30) of artificially contaminating cysts in 30 g of lettuce. We tested the method on a variety of commercially available natural foods, which we also seeded with a commercially available internal control, immediately prior to concentration of the extract. Recoveries of the Texas Red-stained Giardia cyst and Cryptosporidium oocyst internal controls were 36.5% ± 14.3% and 36.2% ± 19.7% (n = 20), respectively. One natural food sample of organic watercress, spinach, and rocket salad contained one Giardia cyst 50 g−1 of sample as an indigenous surface contaminant.

Evaluation of fluorogenic TSC agar for recovering Clostridium perfringens in groundwater samples

2001 ◽  
Vol 43 (12) ◽  
pp. 201-204 ◽  
Author(s):  
M. Araujo ◽  
R. A. Sueiro ◽  
M. J. Gómez ◽  
M. J. Garrido
Keyword(s):  
Water Pollution ◽  
Clostridium Perfringens ◽  
Membrane Filtration ◽  
The Other ◽  
Human Consumption ◽  
Recovery Efficiency ◽  
Groundwater Samples ◽  
Pollution Indicator ◽  
The Media ◽  
Filtration Technique

Clostridium perfringens is widely recognised as a reliable water pollution indicator. Since several media can be employed for the membrane filtration enumeration of this microorganism, the main aim of this work was to investigate the ability of fluorocult-supplemented TSC-agar (Merck) for recovering Cl. perfringens from public springs used for direct human consumption. Cl. perfringens recovery was also performed on mCP agar (Cultimed) according to Directive 98/83 as well as on TSC-Agar (Merck), TSN-Agar (Merck) and SPS-Agar (BBL) media. Variance analysis of data obtained showed no statistically significant differences in the counts obtained among all media employed in this work. However, the Cl. perfringens recovery efficiencies with TSC and fluorogenic TSC agars were significantly greater (P = < 0.05) than the corresponding values of mCP and TSN media. On the other hand, the identification of typical and atypical colonies isolated from all media demonstrated that fluorogenic TSC agar was the most specific medium for Cl. perfringens recovery in groundwater samples (85.3% of typical colonies and 82.8% of atypical colonies confirmed). In summary, the membrane filtration technique with fluorogenic TSC agar showed the best performance characteristics of all the media tested as judged by their recovery efficiency and specificity in these water samples.

scholarly journals Detection of Giardia and Cryptosporidium cysts/oocysts in watersheds and drinking water sources in Brazil urban areas

2009 ◽  
Vol 8 (2) ◽  
pp. 399-404 ◽  
Author(s):  
Maria Tereza Pepe Razzolini ◽  
Thaís Filomena da Silva Santos ◽  
Veridiana Karmann Bastos
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Urban Areas ◽  
Water Sources ◽  
Raw Water ◽  
Protozoan Parasites ◽  
The Us ◽  
Cryptosporidium Oocysts ◽  
Us Epa ◽  
Giardia Cysts

The protozoan parasites Giardia and Cryptosporidium have been described as important waterborne disease pathogens, and are associated with severe gastrointestinal illnesses. The objective of this paper was to investigate the presence of Giardia cysts and Cryptosporidium oocysts in samples from watershed catchments and treated water sources. A total of 25 water samples were collected and examined according to the US EPA—Method 1623, 2005, consisting of 12 from drinking water and 13 from raw water. Positive samples from raw water for Giardia cysts and Cryptosporidium oocysts were 46.1 and 7.6%, respectively. In finished water, positive samples were 41.7% for Giardia cysts and 25.0% for Cryptosporidium oocysts. Concentrations of Giardia cysts found in raw water samples ranged from “not detected” to 3.4 cysts/L, whereas concentrations of Cryptoporidium oocysts ranged from “not detected” to 0.1 oocysts/L. In finished water, Giardia concentrations ranged from “not detected” to 0.06 cysts/L, and Cryptosporidium, from “not detected” to 0.01 oocysts/L. Concentrations of Giardia cysts and Cryptosporidium oocysts were not high in the samples analyzed. Nevertheless, the results of this study highlight the need to monitor these organisms in both raw and drinking water.

Isolation and Enumeration of Giardia Cysts, Cryptosporidium Oocysts, and Ascaris Eggs from Fruits and Vegetables

2000 ◽  
Vol 63 (6) ◽  
pp. 775-778 ◽  
Author(s):  
L. J. ROBERTSON ◽  
B. GJERDE
Keyword(s):  
Fruits And Vegetables ◽  
Immunomagnetic Separation ◽  
Fruit And Vegetables ◽  
Cryptosporidium Oocysts ◽  
Brightfield Microscopy ◽  
Bean Sprouts ◽  
Giardia Cysts

Published techniques for recovering parasites from fruit and vegetables are generally inadequate, with low and variable recovery efficiencies. Here we describe an improved methodology for analyzing fruit and vegetables for Giardia cysts, Cryptosporidium oocysts, and Ascaris eggs. The method includes washing procedures, sonication, and, for Giardia and Cryptosporidium, immunomagnetic separation. Identification is by immunofluorescence (Giardia and Cryptosporidium) or brightfield microscopy (Ascaris). Recovery efficiencies from lettuce, Chinese leaves, and strawberries were found to be approximately 67% for Giardia, 42% for Cryptosporidium, and 72% for Ascaris. Recovery efficiencies from bean sprouts tended to be more variable and lower. This could be due to material removed with the parasites during the washing procedures, which, in turn, appeared related to the age of the bean sprouts. It is therefore recommended that fruit and vegetables should be as fresh as possible when analyzed for parasites.

Factors Affecting Recovery Efficiency in Isolation of Cryptosporidium Oocysts and Giardia Cysts from Vegetables for Standard Method Development

2001 ◽  
Vol 64 (11) ◽  
pp. 1799-1805 ◽  
Author(s):  
L. J. ROBERTSON ◽  
B. GJERDE
Keyword(s):  
Sample Size ◽  
Standard Method ◽  
Fruits And Vegetables ◽  
Method Development ◽  
Immunomagnetic Separation ◽  
Recovery Efficiency ◽  
Factors Affecting ◽  
Cryptosporidium Oocysts ◽  
Magnetic Strength ◽  
Bean Sprouts

While recently published techniques for recovering parasites from fruits and vegetables demonstrate a marked increase in efficiency and utility, there is still scope for further improvement in developing a standard method, particularly with difficult, but important, sample matrices such as bean sprouts. Herein, a number of parameters used in published techniques are investigated more closely. While sample size reduction may improve recovery efficiency because of a range of factors, it is important to keep the sample large enough for detection of low-level contamination. Age of sample is also important, and samples should be as fresh as possible. Elution procedures may contribute to losses of Giardia and should be more thoroughly investigated. Improved immunomagnetic separation techniques currently coming onto the market also have the potential to increase recovery efficiency substantially, even with difficult samples such as aged bean sprouts. However, merely increasing magnetic strength of the capturing magnet does not affect recovery efficiency, which must be reliant on a superior bead system, buffering system, or both.

Pilot-plant study of a high recovery membrane filtration process for drinking water treatment

2000 ◽  
Vol 41 (10-11) ◽  
pp. 77-84 ◽  
Author(s):  
J.Y. Huang ◽  
S. Takizawa ◽  
K. Fujita
Keyword(s):  
Membrane Fouling ◽  
Membrane Filtration ◽  
Cross Flow ◽  
Drinking Water Treatment ◽  
Pilot Scale ◽  
Raw Water ◽  
Circulation Flow ◽  
High Recovery ◽  
Chemical Cleaning ◽  
Cross Flow Filtration

Successful application of energy efficient dead-end micro-filtration (MF), which does not require recirculation like cross-flow filtration, depends on achieving high recovery rates. In this study, two different types of pilot scale membrane systems (horizontally and vertically stretched membrane filters) were evaluated based on the effects of pre-chlorination, intermittent chlorination and circulation flow by air-scrubbing. Additionally, the effects of operating factors including physical cleaning and chemical cleaning on membrane fouling were examined. The vertically stretched membranes showed better performance than horizontally stretched membranes at filtration fluxes of either 0.55 m/day or 0.78 m/day even under 2.6 to 27.5°C and raw water turbidity higher than 300 units, as long as intermittent chlorination (10 mg/l once a week) along with the circulation flow by air-scrubbing (once in 30 minutes) in the housing were employed. It was demonstrated that the vertically stretched membranes have been operated for one year without chemical cleaning of the membranes, wherein the recovery of raw water as a filtrate was 97.0% or 98.9%.

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