Production and monomer composition of exopolysaccharides by yogurt starter cultures

2000 ◽  
Vol 46 (12) ◽  
pp. 1123-1127 ◽  
Author(s):  
Ginka I Frengova ◽  
Emilina D Simova ◽  
Dora M Beshkova ◽  
Zhelyasko I Simov
Keyword(s):  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Lactobacillus Bulgaricus ◽  
Streptococcus Salivarius ◽  
Monomer Composition ◽  
Monomer Structure ◽  
Production Activity ◽  
Thermophilic Streptococci ◽  
Lactobacillus Delbrueckii Subsp

As components of starter cultures for Bulgarian yogurt, Streptococcus salivarius subsp. thermophilus and Lactobacillus delbrueckii subsp. bulgaricus revealed extensive exopolysaccharide (EPS) production activity when cultivated in whole cow's milk. The polymer-forming activity of thermophilic streptococci was lower (230-270 mg EPS/L) than that of the lactobacilli (400-540 mg EPS/L). Mixed cultures stimulated EPS production in yogurt manufacture, and a maximum concentration of 720-860 mg EPS/L was recorded after full coagulation of milk. The monomer structure of the exopolysaccharides formed by the yogurt starter cultures principally consists of galactose and glucose (1:1), with small amounts of xylose, arabinose, and/or mannose.Key words: Streptococcus thermophilus, Lactobacillus bulgaricus, starter cultures, yogurt, exopolysaccharides.

Production of natural folates by lactic acid bacteria starter cultures isolated from artisanal Argentinean yogurts

2012 ◽  
Vol 58 (5) ◽  
pp. 581-588 ◽  
Author(s):  
Jonathan Emiliano Laiño ◽  
Jean Guy LeBlanc ◽  
Graciela Savoy de Giori
Keyword(s):  
Lactic Acid ◽  
Folic Acid ◽  
Lactic Acid Bacteria ◽  
Culture Medium ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Nonfat Milk ◽  
Native Strains ◽  
Lactobacillus Delbrueckii Subsp

Folate is a B-group vitamin that cannot be synthesized by humans and must be obtained exogenously. Although some species of lactic acid bacteria (LAB) can produce folates, little is known about the production of this vitamin by yogurt starter cultures. Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains were isolated from artisanal Argentinean yogurts and were grown in folate-free culture medium (FACM) and nonfat milk after which intracellular and extracellular folate production were evaluated. From the initial 92 isolated LAB strains, 4 L. delbrueckii subsp. bulgaricus and 32 S. thermophilus were able to grow in the absence of folate. Lactobacillus delbrueckii subsp. bulgaricus CRL 863 and S. thermophilus CRL 415 and CRL 803 produced the highest extracellular folate levels (from 22.3 to 135 µg/L) in FACM. In nonfat milk, these strains were able to increase the initial folate concentrations by almost 190%. This is the first report where native strains of L. delbrueckii subsp. bulgaricus were shown to produce natural folate. The LAB strains identified in this study could be used in developing novel fermented products bio-enriched in natural folates that could in turn be used as an alternative to fortification with the controversial synthetic chemical folic acid.

INVESTIGATION OF THE RHEOLOGICAL CHARACTERISTICS AND LEVEL OF SYNTHESIS OF EXOPOLYSACCHARIDES (EPS) BY THE CONSORTIUM OF STREPTOCOCCUS SALIVARIUS SUBSP. THERMOPHILUS AND LACTOBACILLUS DELBRUECKII SUBSP. BULGARICUS AT DIFFERENT TEMPERATURE IN PROCESS OF MILK FERMENTATION

2020 ◽  
pp. 58-67
Author(s):  
O. Golovach ◽  
M. Babitskaya ◽  
N. Zhabanos ◽  
I. Pyzhik ◽  
M. Korkina ◽  
...  
Keyword(s):  
Raw Materials ◽  
Skim Milk ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Streptococcus Salivarius ◽  
Temperature Conditions ◽  
Fermentation Temperature ◽  
Organoleptic Characteristics ◽  
Milk Fermentation ◽  
Lactobacillus Delbrueckii Subsp

The article provides the results of studies on the influence of temperature conditions of milk fermentation in the manufacture of yogurt on the characteristics of milk clots and the level of EPS production. The characteristics of the samples of reconstituted skim milk fermented by consortia of lactic acid microorganisms for the manufacture of starter and viscous consistency yoghurt starter cultures under temperature conditions are determined: (43 ± 1)°С, (38 ± 1)°С, (35 ± 1)°С and ( 30 ± 1)°С. It was noted that with a decrease in the fermentation temperature from (43±1)°C to (30±1)°С, the organoleptic characteristics of the formed clot are evaluated differently. At the fermentation temperature of (30±1)°С, the lowest degree of syneresis was noted: 45% for the consortium, during the fermentation of milk raw materials forming clumps of inviscid consistency, 29% for the consortium, during the fermentation of milk raw materials forming clumps of viscous consistency. At the same time, it was found that at fermentation temperatures of (43±1)°С and (30±1)°С, the highest level of EPS synthesis for consortia was noted: (2107 ST-A+2674 TL-AV) – 874.6 and 667.9 μg / ml, (1141 ST-AV+2674 TLAV) – 683.9 and 541.3 μg / ml.

scholarly journals Biodiversity and Safety Assessment of Half-Century Preserved Natural Starter Cultures for Pecorino Romano PDO Cheese

2021 ◽  
Vol 9 (7) ◽  
pp. 1363
Author(s):  
Luigi Chessa ◽  
Antonio Paba ◽  
Elisabetta Daga ◽  
Ilaria Dupré ◽  
Roberta Comunian
Keyword(s):  
Enterococcus Faecium ◽  
Safety Assessment ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Strain Level ◽  
New Approach ◽  
The 1960S ◽  
Product Specification ◽  
Lactobacillus Delbrueckii Subsp

The use of biodiverse autochthonous natural starter cultures to produce typical and PDO cheeses contributes to establishing a link between products and territory of production, which commercial starters, constituted by few species and strains, are not able to. The purpose of this work was the assessment of biodiversity, at strain level, and safety of natural scotta-innesto cultures whose use is mandatory for the Pecorino Romano PDO cheese manufacturing, according to its product specification. The biodiversity of three scotta-innesto, collected in the 1960s and preserved in lyophilised form, was assessed by molecular biotyping using both PFGE and (GTG)5 rep-PCR profiling on 209 isolates belonging to Streptococcus thermophilus (30), Lactobacillus delbrueckii subsp. lactis (72), Enterococcus faecium (87), and Limosilactobacillus reuteri (20), revealing high biodiversity, at the strain level, in the cultures. The cultures’ safety was proved through a new approach assessing phenotypic and molecular antibiotic resistance of the cultures in toto, instead of single strains, while the safety of Enterococcus faecium isolates was investigated according to EFSA guidelines. The use of natural biodiverse cultures for the production of microbial starters for typical and PDO cheeses, such as Pecorino Romano, could be an opportunity for recovering the cheese microbiota biodiversity lost during years of commercial starters use.

scholarly journals Research of rheological properties of fermented milk ice cream with high content of exopolysaccharides

2021 ◽  
Vol 30 ◽  
pp. 01015
Author(s):  
Ekaterina Pozhidaeva ◽  
Lyubov Golubeva ◽  
Anton Sadchenko ◽  
Yana Dymovskih
Keyword(s):  
Starter Culture ◽  
Control Sample ◽  
Ice Cream ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Lactobacillus Bulgaricus ◽  
Thawing Rate ◽  
Milk Mixture

As a result of scientific and technological research, the ingredient composition and the feasibility of using complex bacterial starter cultures as part of fermented milk ice cream have been substantiated. The objects of the study were samples of mixtures for the production of fermented milk ice cream were considered, which included dairy and non-dairy components, including complex bacterial starter cultures: YF-L812 (Streptococcus thermophilus, Lactobacillus bulgaricus) - control sample and YO-PROX 777 (Streptococcus thermophilus, Lactobacillus delbrueckii ssp.bulgaricus), as well as pectin stabilizers “Grindsted Pektin LC 710” and “Cremodan SE 334” with a concentration of 0.1-0.7%. It was found that a prototype sample with starter culture YO-PROX 777 has increased values of dynamic viscosity compared with the control sample with similar stabilizers and their identical concentrations, which is evidence of the accumulation of exopolysaccharides in the fermented fermented milk mixture. A prototype of fermented milk ice cream has better shape stability during temperature control, the thawing rate is reduced by 1.6 times compared to the control. The degree of overrun of the prototype fermented milk ice cream is 37.6%, which is 1.2 times higher compared to the control.

Exopolysaccharides Produced by Lactic Acid Bacteria of Kefir Grains

2002 ◽  
Vol 57 (9-10) ◽  
pp. 805-810 ◽  
Author(s):  
Ginka I. Frengova ◽  
Emilina D. Simova ◽  
Dora M. Beshkova ◽  
Zhelyasko I. Simov
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Starter Culture ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Helveticus ◽  
Lactobacillus Delbrueckii ◽  
Monomer Composition ◽  
Kefir Grains ◽  
The One ◽  
Lactobacillus Delbrueckii Subsp

A Lactobacillus delbrueckii subsp. bulgaricus HP1 strain with high exopolysaccharide activity was selected from among 40 strains of lactic acid bacteria, isolated from kefir grains. By associating the Lactobacillus delbrueckii subsp. bulgaricus HP1 strain with Streptococcus thermophilus T15, Lactococcus lactis subsp. lactis C15, Lactobacillus helveticus MP12. and Sacharomyces cerevisiae A13, a kefir starter was formed. The associated cultivation of the lactobacteria and yeast had a positive effect on the exopolysaccharide activity of Lactobacillus delbrueckii subsp. bulgaricus HP1. The maximum exopolysaccharide concentration of the starter culture exceeded the one by the Lactobacillus delbrueckii subsp. bulgaricus HP1 monoculture by approximately 1.7 times, and the time needed to reach the maximum concentration (824.3 mg exopolysacharides/l) was shortened by 6 h. The monomer composition of the exopolysaccharides from the kefir starter culture was represented by glucose and galactose in a 1.0:0.94 ratio, which proves that the polymer synthesized is kefiran.

Yeasts as Primary Contaminants in Yogurts Produced Commercially in Lagos, Nigeria

1987 ◽  
Vol 50 (3) ◽  
pp. 193-198 ◽  
Author(s):  
MINABELEMA DUBLIN GREEN ◽  
STELLA N. IBE
Keyword(s):  
Streptococcus Thermophilus ◽  
Maximum Growth ◽  
Starter Cultures ◽  
Lactobacillus Bulgaricus ◽  
Contamination Level ◽  
Psychrotrophic Bacteria ◽  
Retail Outlets ◽  
Candida Lusitaniae ◽  
Aspergillus Sp ◽  
Initial Contamination

Yogurt samples purchased from a dairy industry, retail outlets, and hawkers in Lagos, Nigeria, were plated on potato dextrose agar containing 100 μg of chloramphenicol/ml and found to contain Candida lusitaniae, C krusei, C rugosa, Kluveromyces fragilis, and Saccharomyces cerevisiae as the primary yeast contaminants. Sixty-seven percent of the samples had yeast counts in the range of 104–106 cfu/ml. C. lusitaniae, K. fragilis and C. krusei had the highest counts and occurrence of 65, 48 and 51%, respectively, in 100 random yogurt samples. C. lusitaniae attained the highest count in yogurts stored at 10°C and C. krusei in those at 30°C. Ninety percent of the samples had less than 10 coliforms or staphylococci per ml, whereas 20% had over 103 psychrotrophic bacteria per ml. Molds of Aspergillus sp. and Neurospora sp. were isolated mainly from the strawberry-fluid yogurt. The starter cultures, Lactobacillus bulgaricus and Streptococcus thermophilus, were present in the ratio of 1:1 and reached maximum growth levels of 107 to 108 cfu/ml after 4 to 8 d of storage at 10°C, whereas the yeast continued to increase beyond this level by the 12th day. The extent of contamination observed suggests high initial contamination level and improper refrigeration of yogurts marketed.

scholarly journals CHARACTERIZATION OF HAZELNUT MILK FERMENTED BY LACTOBACILLUS DELBRUECKII SUBSP. BULGARICUS AND STREPTOCOCCUS THERMOPHILUS

2018 ◽  
pp. 677-686 ◽  
Author(s):  
Ertan Ermiş ◽  
Rabia Güneş ◽  
İnci Zent ◽  
Muhammed Yusuf Çağlar ◽  
Mustafa Tahsin Yılmaz
Keyword(s):  
Streptococcus Thermophilus ◽  
Lactobacillus Delbrueckii ◽  
Lactobacillus Delbrueckii Subsp
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