New antifungal metabolites from the coprophilous fungus Cercophorasordarioides

1997 ◽  
Vol 75 (6) ◽  
pp. 768-772 ◽  
Author(s):  
Authrine C. Whyte ◽  
Katherine B. Gloer ◽  
James B. Gloer ◽  
Brenda Koster ◽  
David Malloch
Keyword(s):  
Ethyl Acetate ◽  
Key Words ◽  
Natural Product ◽  
Fungal Metabolite ◽  
Liquid Cultures ◽  
Antifungal Metabolites ◽  
Coprophilous Fungus ◽  
Bioassay Guided Fractionation ◽  
Related Compounds ◽  
Ethyl Acetate Extracts

A chemical investigation of the coprophilous fungus Cercophorasordarioides has led to the isolation of arthrinone (1), a known fungal metabolite, and three new related compounds: 1-dehydroxyarthrinone (2), 3a,9a-deoxy-3a-hydroxy-1-dehydroxyarthrinone (3), and cerdarin (4). These metabolites were obtained from antifungal ethyl acetate extracts of liquid cultures of C. sordarioides through bioassay-guided fractionation, and their structures were assigned on the basis of 1D-NMR, HMQC, and HMBC results. Compounds 2 and 4 exhibited anti-Candida activity. Key words: antifungal, fungal metabolite, natural product, Cercophorasordarioides.

scholarly journals Metabolite Profiles of Lactic Acid Bacteria in Grass Silage

2007 ◽  
Vol 73 (17) ◽  
pp. 5547-5552 ◽  
Author(s):  
Anders Broberg ◽  
Karin Jacobsson ◽  
Katrin Ström ◽  
Johan Schnürer
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pichia Anomala ◽  
Coumaric Acid ◽  
Phenyllactic Acid ◽  
Liquid Cultures ◽  
Antifungal Metabolites ◽  
Metabolite Profiles ◽  
Bioassay Guided Fractionation ◽  
Test Organisms

ABSTRACT The metabolite production of lactic acid bacteria (LAB) on silage was investigated. The aim was to compare the production of antifungal metabolites in silage with the production in liquid cultures previously studied in our laboratory. The following metabolites were found to be present at elevated concentrations in silos inoculated with LAB strains: 3-hydroxydecanoic acid, 2-hydroxy-4-methylpentanoic acid, benzoic acid, catechol, hydrocinnamic acid, salicylic acid, 3-phenyllactic acid, 4-hydroxybenzoic acid, (trans, trans)-3,4-dihydroxycyclohexane-1-carboxylic acid, p-hydrocoumaric acid, vanillic acid, azelaic acid, hydroferulic acid, p-coumaric acid, hydrocaffeic acid, ferulic acid, and caffeic acid. Among these metabolites, the antifungal compounds 3-phenyllactic acid and 3-hydroxydecanoic acid were previously isolated in our laboratory from liquid cultures of the same LAB strains by bioassay-guided fractionation. It was concluded that other metabolites, e.g., p-hydrocoumaric acid, hydroferulic acid, and p-coumaric acid, were released from the grass by the added LAB strains. The antifungal activities of the identified metabolites in 100 mM lactic acid were investigated. The MICs against Pichia anomala, Penicillium roqueforti, and Aspergillus fumigatus were determined, and 3-hydroxydecanoic acid showed the lowest MIC (0.1 mg ml−1 for two of the three test organisms).

scholarly journals Antimicrobial activity of n-hexane and Ethyl acetate extracts of Erythrina stricta Roxb

1970 ◽  
Vol 27 (2) ◽  
pp. 65-66
Author(s):  
Mohammad Musarraf Hussain ◽  
M Mizanur Rahman Mughal ◽  
Md Masud Alam ◽  
Mohammad Golam Dastagir ◽  
AHM Masum Billah ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Key Words ◽  
Microbial Growth ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Zone Of Inhibition ◽  
Microbiological Investigation ◽  
Ethyl Acetate Extracts

The crude n-hexane and ethyl acetate extract of the stem bark of E. stricta were subjected to microbiological investigation and were found to be significantly inhibitory to microbial growth, with the average zone of inhibition 12–17 and 10–16 mm, respectively. In the cytotoxic observation, the n-hexane and ethyl acetate extracts were found to show LC50 of 2.1 and 0.316 mg/ml respectively. Key words: Erythrina stricta; Fabaceae; Antimicrobial activities; Cytotoxicity observation. DOI: http://dx.doi.org/10.3329/bjm.v27i2.9176 BJM 2010; 27(2): 65-66

scholarly journals Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts fromCarica papayaLeaves

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Paula Melariri ◽  
William Campbell ◽  
Paschal Etusim ◽  
Peter Smith
Keyword(s):  
Ethyl Acetate ◽  
Carica Papaya ◽  
High Selectivity ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
Selectivity Index ◽  
Antiplasmodial Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioassay Guided Fractionation ◽  
Ethyl Acetate Extracts

We investigated the antiplasmodial properties of crude extracts fromCarica papayaleaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract.C. papayashowed a high selectivity forP. falciparumagainst CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chloroquine-resistant DD2 strains, respectively.Carica papayaethyl acetate extract was subjected to bioassay-guided fractionation to ascertain the most active fraction, which was purified and identified using high-pressure liquid chromatography (HPLC) and GC-MS (Gas chromatography-Mass spectrometry) methods. Linoleic and linolenic acids identified from the ethyl acetate fraction showed IC50of 6.88 μg/ml and 3.58 μg/ml, respectively. The study demonstrated greater antiplasmodial activity of the crude ethyl acetate extract ofCarica papayaleaves with an IC50of2.96±0.14 μg/ml when compared to the activity of the fractions and isolated compounds.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

α-glucosidase Inhibitory Activity of Extracts and Compounds from the Leaves of Ruellia tuberosa L.

2021 ◽  
Vol 11 ◽  
Author(s):  
Le Nguyen Tuong Vi ◽  
Nguyen Ngoc Tuan ◽  
Quach Tong Hung ◽  
Pham Thi Nhat Trinh ◽  
Tong Thanh Danh ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Spectroscopic Method ◽  
Positive Control ◽  
Strong Activity ◽  
Butanol Extract ◽  
Glucosidase Inhibitor ◽  
Inhibitory Activities ◽  
Bio Assay ◽  
Ethyl Acetate Extracts

Background: In recent years, the study of the structure and biological activity of medicinal plants has a particularly important to search for diabetes medicine. Ruellia tuberosa is used to treat various diseases such as diabetes by inhibiting the activity of α-glucosidase. Objective: In this study, experiment was designed to isolated isolate and identified identify α-glucosidase inhibitory extracts and compounds from Ruellia tuberosa L. through bio-assay guided isolation. Method: Dry powder of Ruellia tuberosa L. was extracted with 70% ethanol, then liquid-liquid partition with n-hexane, ethyl acetate and butanol, respectively. The extracts were evaluated with α-glucosidase inhibition. The potential extracts were isolated and identified compounds. The effects of these compounds on the α-glucosidase inhibitory were evaluated. Results: The a-glucosidase inhibitory activities showed that the n-hexane, ethyl acetate and the butanol extract had the α-glucosidase inhibition with an IC50 of 46.2 0.9, 6.6 2.9 and 8.9  μg/mL, respectively. From the n-hexane and ethyl acetate extracts, the structures of four compounds were elucidated by NMR spectroscopic method, including lupeol (1), syringaresinol (2), apigenin (3), verbascoside (4). The a-glucosidase inhibitory activities showed that all isolated compounds were more active than the positive control - acarbose with an IC50 of 37.5  0.4; 19.5  0.2; 20.1  0.3; 69.3  0.2 µg/mL, respectively. Conclusion: The ethyl acetate extract showed strong activity about 19 times more than positive control - acarbose. The compound syringaresinol (2) was the most powerful α-glucosidase inhibitor. Therefore, Ruellia tuberosa L. contains many compounds that can inhibit α-glucosidase activity.

scholarly journals Toxicity of Thevetia peruviana (Pers) Schum. Extract to Adults of Callosobruchus maculatus F. (Coleoptera: Bruchidae)

1970 ◽  
pp. 105-109 ◽  
Author(s):  
JU Mollah ◽  
W Islam
Keyword(s):  
Ethyl Acetate ◽  
Key Words ◽  
Organic Solvents ◽  
Callosobruchus Maculatus ◽  
Root Extract ◽  
Thevetia Peruviana

Leaf, stem and roots of Thevetia peruviana (Pers) Schum. were extracted in four organic solvents; petroleum spirit, ethyl acetate, acetone and methanol and tested against the adults of Callosobruchus maculatus F. All the tested extracts effectively produced mortality of C. maculatus and their toxicity was in order of solvents: petroleum spirit>ethyl acetate>acetone>methanol. Root extract was the most toxic to C. maculatus. Females were more tolerant than males. Key words: Extract, mortality, solvent, Thevetia peruviana, Callosobruchus maculatus. DOI = 10.3329/jard.v5i1.1466 J Agric Rural Dev 5(1&2), 105-109, June 2007

scholarly journals Kenganthranol F, a new Anthranol from Psorospermum aurantiacum

2013 ◽  
Vol 8 (1) ◽  
pp. 1934578X1300800
Author(s):  
Gesquiere M. Tiani ◽  
Ishtiaq Ahmed ◽  
Karsten Krohn ◽  
Ivan R. Green ◽  
Augustin E. Nkengfack
Keyword(s):  
Ethyl Acetate ◽  
Whole Plant ◽  
Ethyl Acetate Extracts

A new anthranol, kenganthranol F, was isolated from the ethyl acetate extracts of the seeds and the whole plant of the Cameroonian plant Psorospermum aurantiacum (Hypericaceae), together with fifteen known compounds viz., ferruginin B, vismin, vismion D, haronginanthrone, kenganthraquinone, kenganthranol B and kenganthranol E isolated from the fruits and 3-geranyloxyemodinanthrone, 2-geranylemodin, bianthrone A1, vismione D, 1,8-dihydroxy- 3-geranyloxy-6-methylanthraquinone, vismione M, vismiaquinone, vismiaquinone C being isolated from the whole plant. The structure of the new isomer of kenganthranol F was determined to be 1,8,10-trihydroxy-3,4-[2,2-dimethyldihydropyrano]-6-methyl-2,5-bis-(3,3-dimethylallyl)-anthrone.

scholarly journals Chemical composition and screening of the antimicrobial and anti-oxidative activity of extracts of Stachys species

2010 ◽  
Vol 75 (10) ◽  
pp. 1347-1359 ◽  
Author(s):  
Jelena Lazarevic ◽  
Radosav Palic ◽  
Niko Radulovic ◽  
Novica Ristic ◽  
Gordana Stojanovic
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Salmonella Enteritidis ◽  
Balkan Peninsula ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Diethyl Ether Extract ◽  
The Common ◽  
Ethyl Acetate Extracts

GC and GC/MS analyses of the diethyl ether and ethyl acetate extracts obtained from the aerial parts of Stachys germanica subsp. heldreichii (Boiss) Hayek, Stachys iva Griseb., Stachys plumosa Griseb. and Stachys scardica Griseb., Balkan peninsula endemics, were performed. One hundred and seventy-nine constituents, accounting for 88.8-98.1% of the total composition of the extracts, were identified. The common feature of the diethyl ether extracts was the high content of terpenoids and fatty acidderived compounds, while the common feature of the ethyl acetate extracts was the prevalence of fatty acid-derived compounds. A disk diffusion method was used for the evaluation of the antimicrobial activities of the extracts against a panel of microorganisms (bacteria: Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella enteritidis; fungi: Aspergillus niger and Candida albicans). The total antioxidant capacity of the extracts was evaluated by the phosphomolybdenum method. The preliminary bioassay results indicated that the diethyl ether extract of S. plumosa could be a possible source of antioxidant and antimicrobial compounds.

Vasodilatory effects and underlying mechanisms of the ethyl acetate extracts from Gastrodia elata

2017 ◽  
Vol 95 (5) ◽  
pp. 564-571 ◽  
Author(s):  
Rong Dai ◽  
Ting Wang ◽  
Xiaoqin Si ◽  
Yuanyuan Jia ◽  
Lili Wang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Vascular Tone ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Rat Aorta ◽  
Gastrodia Elata ◽  
Vasodilatory Effect ◽  
Intracellular Ca ◽  
Underlying Mechanisms ◽  
Ethyl Acetate Extracts

The objective of this study was to assess the ethyl acetate extracts of Gastrodia elata Blume (GEB) on vascular tone and the mechanisms involved. GEB was extracted with 95% EtOH followed by a further extraction with ethyl acetate. The effects of GEB and its ingredients on the isometric tensions of the aortic rings from rats were measured. The ethyl acetate extract of GEB induced a vasodilatory effect on rat aorta, which was partially dependent on endothelium. Four chemical compounds isolated from GEB were identified as 3,4-dihydroxybenzaldehyde (DB), 4-hydroxybenzaldehyde (HB), 4-methoxybenzyl alcohol (MA), and 4,4′-dihydroxydiphenyl methane (DM), respectively. All of these compounds induced vasodilatations, which were dependent on the endothelium to different degrees. After pretreatment with Nω-nitro-l-arginine methyl ester, indomethacin, or methylene blue, the vasodilatations induced by DB, HB, and MA were significantly decreased. In addition, the contractions of the rat aortic rings due to Ca2+ influx and intracellular Ca2+ release were also inhibited by DM. Furthermore, the administration of DB significantly enhanced the productions of nitric oxide (NO) and the activities of the endothelial NO synthase in aorta and in endothelial cells. Thus, GEB may play an important role in the amelioration of hypertension by modulating vascular tones.

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