Structural studies of the capsular polysaccharide from Streptococcus pneumoniae Type 12F

1981 ◽  
Vol 59 (14) ◽  
pp. 2081-2085 ◽  
Author(s):  
Karin Leontein ◽  
Bengt Lindberg ◽  
Jörgen Lönngren
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nmr Spectroscopy ◽  
Capsular Polysaccharide ◽  
Structural Studies ◽  
Methylation Analysis ◽  
Mannuronic Acid ◽  
Structure Formula

The capsular polysaccharide from Streptococcus pneumoniae type 12F is composed of D-glucosyl, D-galactosyl, 2-acetamido-2-deoxy-D-galactosyl, 2-acetamido-2,6-dideoxy-L-galactosyl, and 2-acetamido-2-deoxy-D-mannuronic acid residues in the proportions 2:1:1:1:1. The main structural evidence was adduced from nmr spectroscopy, methylation analysis, and specific degradations whereby it could be concluded that the polysaccharide is composed of hexasaccharide repeating-units having the structure:[Formula: see text]

Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype 2

1987 ◽  
Vol 65 (5) ◽  
pp. 414-422 ◽  
Author(s):  
Eleonora Altman ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Structural Studies ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Structure Formula ◽  
Nuclear Magnetic

The capsular polysaccharide of Haemophilus pleuropneumoniae serotype 2 (ATCC 27089) is composed of D-glucose (two parts), D-galactose (one part), glycerol (one part), and phosphate (one part). Hydrolysis, dephosphorylation, methylation, enzymic studies, and 1H and 13C nuclear magnetic resonance experiments showed that the polysaccharide is a high molecular weight polymer of a tetrasaccharide repeating units, linked by monophosphate diester and having the following structure:[Formula: see text]

Structural Studies on the Galactomannans of Lichens of the Genus Cladonia

1997 ◽  
Vol 29 (5) ◽  
pp. 471-481 ◽  
Author(s):  
S. M. Woranovicz ◽  
P. A. J. Gorin ◽  
M. P. Marcelli ◽  
G. Torri ◽  
M. Iacomini*
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr ◽  
13C Nmr Spectroscopy ◽  
Lichen Species ◽  
Chemical Data ◽  
Alkaline Extraction ◽  
Structural Studies ◽  
Methylation Analysis ◽  
Smith Degradation

AbstractGalactomannans were isolated from Cladonia signata, C. furcata, C. imperialis, and C. clathrata via successive alkaline extraction and precipitation with Fehling solution and Cetavlon. They were investigated using ⊃13C-NMR spectroscopy, methylation analysis, and Smith degradation, and their specific rotations and monosaccharide compositions determined. As with galactomannans of other Cladonia species, they contained (l →6)-linked main chains of α-mannopyranose, which were non-substituted (structure 4 in Fig. 2), monosubstituted at O-2 with α-mannopyranose (structure 6) or α-galactopyranose (structure 1), O-4 with β-galactopyranose (structure 2), and disubstituted at O-2 and O-4 with α-mannopyranosyl and β-galactopyranosyl units, respectively (structure 5). Disubstitution was present to a greater extent in the galactomannans of C. clathrata and C. imperialis than in those of C. signata and C. furcata. In the case of the galactomannans of C. furcata, C. clathrata, and C. imperialis, substitution also occurred at O-2 with O-β-galactofuranosyl-(l→6)-O-α-mannopyranosyl units (structure 7). As observed in previous investigations, the C-l portion of the ⊃13C-NMR of mannose-containing polysaccharides is typical of the lichen species. However, those of galactomannans of C. imperialis and C. clathrata are almost identical and, although other chemical data showed many structures in common, some differences were evident.

Structural studies on a sulfated polysaccharide from an Arthrobacter sp. by NMR spectroscopy and methylation analysis

1997 ◽  
Vol 305 (2) ◽  
pp. 253-260 ◽  
Author(s):  
Ken-ichi Yamazaki ◽  
Kazuyoshi Inukai ◽  
Makoto Suzuki ◽  
Hiroshi Kuga ◽  
Hiroshi Korenaga
Keyword(s):  
Nmr Spectroscopy ◽  
Sulfated Polysaccharide ◽  
Structural Studies ◽  
Methylation Analysis

Structural analysis of the specific capsular polysaccharide of Streptococcuspneumoniae type 22F

1989 ◽  
Vol 67 (6) ◽  
pp. 1038-1050 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Periodate Oxidation ◽  
Resonance Spectroscopy ◽  
Methylation Analysis ◽  
Chemical Shift Correlation ◽  
Structure Formula ◽  
Correlation Techniques ◽  
Heteronuclear Chemical Shift ◽  
C Nmr

The structure of the specific capsular polysaccharide produced by Streptococcuspneumoniae type 22F (American type 22) was investigated by high-field 1H and 13C nuclear magnetic resonance spectroscopy, composition, methylation analysis, and periodate oxidation studies. The polysaccharide was found to be a high molecular weight acidic polymer composed of D-glucose, D-galactose, D-glucuronic acid, and L-rhamnose residues to form a regular repeating hexasaccharide unit having the structure[Formula: see text]in which the β-L-rhamnopyranosyl residues were substituted by O-acetyl groups in 80% of the repeating units. The 1H and 13C nmr resonances of the O-deacetylated type 22F polysaccharide were completely assigned by application of two-dimensional homo- and heteronuclear chemical shift correlation techniques. Keywords: Streptococcuspneumoniae polysaccharide, NMR analysis.

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30)

1984 ◽  
Vol 62 (2-3) ◽  
pp. 151-161 ◽  
Author(s):  
Martine Caroff ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30) is composed of D-galactose (three parts), D-glucose (one part), 2-acetamido-2-deoxy-D-glucose (one part), phosphate (one part), and glycerol (one part). Hydrolysis, periodate oxidation, methylation, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight linear polymer of a pentasaccharide repeating unit having the structure:[Formula: see text]

Structural studies of an arabinan from the stems of Ephedra sinica by methylation analysis and 1D and 2D NMR spectroscopy

2015 ◽  
Vol 121 ◽  
pp. 449-456 ◽  
Author(s):  
Yong-Gang Xia ◽  
Jun Liang ◽  
Bing-You Yang ◽  
Qiu-Hong Wang ◽  
Hai-Xue Kuang
Keyword(s):  
Nmr Spectroscopy ◽  
2D Nmr ◽  
Structural Studies ◽  
Methylation Analysis ◽  
2D Nmr Spectroscopy ◽  
Ephedra Sinica

Structural studies of the capsular polysaccharide from Streptococcus pneumoniae type 18F

1988 ◽  
Vol 173 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Per-Erik Jansson ◽  
Bengt Lindberg ◽  
Johan Lindberg ◽  
Jørgen Henrichsen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Structural Studies

Structural studies of the capsular polysaccharide from Streptococcus pneumoniae type 17A

1983 ◽  
Vol 118 ◽  
pp. 157-171 ◽  
Author(s):  
Per-Erik Jansson ◽  
Bengt Lindberg ◽  
Ulf Lindquist ◽  
Dennis J. Carlo
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Structural Studies

Structural studies of the capsular polysaccharide from streptococcus pneumoniae type 2, a reinvestigation

1988 ◽  
Vol 182 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Per-Erik Jansson ◽  
Bengt Lindberg ◽  
Mats Andersson ◽  
Ulf Lindquist ◽  
Jørgen Henrichsen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Structural Studies

Structural studies of the capsular polysaccharide from streptococcus pneumoniae type 1

1980 ◽  
Vol 78 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Bengt Lindberg ◽  
Bengt Lindqvist ◽  
Jörgen Lönngren ◽  
David A. Powell
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Structural Studies
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