Kinetic Studies of the Reaction of Picryl Chloride with Sodium Methoxide in Methanol

1974 ◽  
Vol 52 (1) ◽  
pp. 18-24 ◽  
Author(s):  
Leong Huat Gan ◽  
Albert Richard Norris
Keyword(s):  
Kinetic Parameters ◽  
Sodium Methoxide ◽  
Kinetic Studies ◽  
Analogous Reaction ◽  
Picryl Chloride ◽  
Methoxide Ion ◽  
Thermodynamic And Kinetic Parameters

Picryl chloride reacts rapidly with methoxide ion in methanol to form a σ-complex containing methoxide ion attached to the C-3 position of the picryl chloride. Thermodynamic and kinetic parameters characterizing this reaction are presented and compared with corresponding parameters for the analogous reaction of methoxide ion and 2,4,6-trinitroanisole in methanol. Details concerning the much slower formation of the σ-complex containing methoxide ion added to the C-1 position of picryl chloride are also presented.

scholarly journals Real-time kinetic studies of Mycobacterium tuberculosis LexA-DNA interaction

2021 ◽  
Author(s):  
Chitral Chatterjee ◽  
Soneya Majumdar ◽  
Sachin Deshpande ◽  
Deepak Pant ◽  
Saravanan Matheshwaran
Keyword(s):  
Amino Acids ◽  
Mycobacterium Tuberculosis ◽  
Dna Binding ◽  
Kinetic Parameters ◽  
Dna Sequences ◽  
Kinetic Studies ◽  
Dna Interaction ◽  
Damage Repair ◽  
Inducible Genes ◽  
Kinetics Of

Transcriptional repressor, LexA, regulates the “SOS” response, an indispensable bacterial DNA damage repair machinery.  Compared to its E.coli ortholog, LexA from Mycobacterium tuberculosis (Mtb) possesses a unique N-terminal extension of additional 24 amino acids in its DNA binding domain (DBD) and 18 amino acids insertion at its hinge region that connects the DBD to the C-terminal dimerization/autoproteolysis domain. Despite the importance of LexA in “SOS” regulation, Mtb LexA remains poorly characterized and the functional importance of its additional amino acids remained elusive. In addition, the lack of data on kinetic parameters of Mtb LexA-DNA interaction prompted us to perform kinetic analyses of Mtb LexA and its deletion variants using Bio-layer Interferometry (BLI). Mtb LexA is seen to bind to different “SOS” boxes, DNA sequences present in the operator regions of damage-inducible genes, with comparable nanomolar affinity. Deletion of 18 amino acids from the linker region is found to affect DNA binding unlike the deletion of the N-terminal stretch of extra 24 amino acids. The conserved RKG motif has been found to be critical for DNA binding. Overall, this study provides insights into the kinetics of the interaction between Mtb LexA and its target “SOS” boxes. The kinetic parameters obtained for DNA binding of Mtb LexA would be instrumental to clearly understand the mechanism of “SOS” regulation and activation in Mtb.

scholarly journals Thermodynamic and Kinetic Parameters for Calcite Nucleation on Peptoid and Model Scaffolds: A Step toward Nacre Mimicry

2020 ◽  
Vol 20 (6) ◽  
pp. 3762-3771 ◽  
Author(s):  
Anne R. Nielsen ◽  
Stanislav Jelavić ◽  
Daniel Murray ◽  
Behzad Rad ◽  
Martin P. Andersson ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Thermodynamic And Kinetic Parameters

scholarly journals Thermodynamics and kinetics of DNA nanotube polymerization from single-filament measurements

2015 ◽  
Vol 6 (4) ◽  
pp. 2252-2267 ◽  
Author(s):  
Rizal F. Hariadi ◽  
Bernard Yurke ◽  
Erik Winfree
Keyword(s):  
Kinetic Parameters ◽  
Single Filament ◽  
Common Features ◽  
Thermodynamics And Kinetics ◽  
Polymer Models ◽  
Thermodynamic And Kinetic Parameters ◽  
Model Sharing ◽  
Dna Nanotube ◽  
Kinetics Of

Single-filament measurement of the thermodynamic and kinetic parameters of DNA nanotube assembly supports a polymerization/depolymerization model sharing common features with cytoskeletal polymer models.

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