PARTIAL SPECIFIC VOLUME MEASUREMENTS BY DIFFERENTIAL SEDIMENTATION

1959 ◽  
Vol 37 (10) ◽  
pp. 1662-1670 ◽  
Author(s):  
W. G. Martin ◽  
C. A. Winkler ◽  
W. H. Cook
Keyword(s):  
Heavy Water ◽  
Isotopic Exchange ◽  
Partial Specific Volume ◽  
Plasma Albumin ◽  
Bovine Plasma ◽  
Water Media ◽  
Volume Measurements ◽  
Differential Sedimentation ◽  
Specific Volumes ◽  
Magnetic Float

The partial specific volumes of several macromolecules were determined from their sedimentation rates in aqueous and heavy water solutions with results comparable to those obtained by conventional methods. Corrections for the changes in weight due to isotopic exchange were obtained from measurements on small quantities of material with a quartz spiral balance. Measurements of the partial specific volumes of bovine plasma albumin, polyvinyl alcohol, glycine, and triglycylglycine in both aqueous and heavy water media, made with a magnetic float apparatus, indicate that isotopic exchange has no significant effect on the macromolecular volume. The sedimentation method is therefore a micro method that does not require a knowledge of the concentration of macromolecules and which is applicable to mixtures that are resolvable during sedimentation.

THE DETERMINATION OF PARTIAL SPECIFIC VOLUMES BY DIFFERENTIAL SEDIMENTATION

1956 ◽  
Vol 34 (6) ◽  
pp. 809-814 ◽  
Author(s):  
W. G. Martin ◽  
W. H. Cook ◽  
C. A. Winkler
Keyword(s):  
Polyvinyl Alcohol ◽  
Sodium Alginate ◽  
Heavy Water ◽  
Specific Volume ◽  
Partial Specific Volume ◽  
Plasma Albumin ◽  
Bovine Plasma ◽  
Differential Sedimentation ◽  
Specific Volumes

Sedimentation in aqueous and heavy water solutions has been used to determine the partial specific volume of bovine plasma albumin, sodium alginate, and polyvinyl alcohol, resulting in 0.74 ± 0.01 (S.D.), 0.54 ± 0.06, and 0.79 ± 0.01 ml./gm. at 25 °C, respectively. The sedimentation method yields results comparable with those given by conventional methods for macromolecules.

Bovine Platelet Proteins

1969 ◽  
Vol 21 (03) ◽  
pp. 409-418 ◽  
Author(s):  
S Łopaciuk ◽  
N. O Solum
Keyword(s):  
Bovine Serum ◽  
Protein Composition ◽  
Disc Electrophoresis ◽  
Plasma Fibrinogen ◽  
Plasma Albumin ◽  
Precipitin Line ◽  
Bovine Plasma ◽  
Platelet Proteins

Summary1. The protein composition of bovine platelet extracts has been investigated by immunoelectrophoresis and polyacrylamide disc electrophoresis. The information obtained is discussed as a basis for study on platelet fibrinogen.2. With antiserum to platelet proteins 11 precipitin lines were observed 3 of which corresponded electrophoretically to plasma albumin, fibrinogen and γ-globulin. These lines were not seen using the same antiserum absorbed with bovine plasma. The 8 additional lines were still present indicating that they represented specific platelet components. Antiserum to plasma produced the 3 above-mentioned lines, but no others.3. With antiserum to purified bovine plasma fibrinogen 3 precipitin lines were observed. The fibrinogen line was the dominant one. The 2 additional lines did not disappear by absorption of the antiserum with bovine serum nor by incubation of the extracts with thrombin. The latter treatment totally removed the fibrinogen line.4. A non-fibrinogen precipitin line, observed only with the antiserum to platelet extract and positioned in the β2-globulin region, disappeared by the incubation of platelet extracts with thrombin.

scholarly journals An Endonuclease Associated with Bovine Plasma Albumin Fraction

1972 ◽  
Vol 247 (1) ◽  
pp. 193-198
Author(s):  
Motoaki Anai ◽  
Hiroyuki Haraguchi ◽  
Yasuyuki Takagi
Keyword(s):  
Plasma Albumin ◽  
Albumin Fraction ◽  
Bovine Plasma

The Relation of Free Sulfhydryl Groups to Chromatographic Heterogeneity and Polymerization of Bovine Plasma Albumin*

Biochemistry ◽  
1962 ◽  
Vol 1 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Robert W. Hartley ◽  
Elbert A. Peterson ◽  
Herbert A. Sober
Keyword(s):  
Sulfhydryl Groups ◽  
Plasma Albumin ◽  
Bovine Plasma

CD-resolved secondary structure of bovine plasma albumin in acid-induced isomerization*

2009 ◽  
Vol 22 (3) ◽  
pp. 333-340 ◽  
Author(s):  
SEIICHI ERA ◽  
HIROSHI ASHIDA ◽  
SHUNJI NAGAOKA ◽  
HIROSHI INOUYE ◽  
MASARU SOGAMI
Keyword(s):  
Secondary Structure ◽  
Plasma Albumin ◽  
Bovine Plasma

The Aggregation of Bovine Plasma Albumin at Low pH1

1960 ◽  
Vol 82 (14) ◽  
pp. 3741-3745 ◽  
Author(s):  
Edward J. Williams ◽  
Joseph F. Foster
Keyword(s):  
Plasma Albumin ◽  
Bovine Plasma
Sign in / Sign up

Export Citation Format

Share Document