Partial specific volume of bovine plasma albumin in the presence of potassium chloride

1967 ◽  
Vol 71 (12) ◽  
pp. 3717-3721 ◽  
Author(s):  
Margaret J. Hunter
Keyword(s):  
Potassium Chloride ◽  
Specific Volume ◽  
Partial Specific Volume ◽  
Plasma Albumin ◽  
Bovine Plasma

THE DETERMINATION OF PARTIAL SPECIFIC VOLUMES BY DIFFERENTIAL SEDIMENTATION

1956 ◽  
Vol 34 (6) ◽  
pp. 809-814 ◽  
Author(s):  
W. G. Martin ◽  
W. H. Cook ◽  
C. A. Winkler
Keyword(s):  
Polyvinyl Alcohol ◽  
Sodium Alginate ◽  
Heavy Water ◽  
Specific Volume ◽  
Partial Specific Volume ◽  
Plasma Albumin ◽  
Bovine Plasma ◽  
Differential Sedimentation ◽  
Specific Volumes

Sedimentation in aqueous and heavy water solutions has been used to determine the partial specific volume of bovine plasma albumin, sodium alginate, and polyvinyl alcohol, resulting in 0.74 ± 0.01 (S.D.), 0.54 ± 0.06, and 0.79 ± 0.01 ml./gm. at 25 °C, respectively. The sedimentation method yields results comparable with those given by conventional methods for macromolecules.

scholarly journals Further evidence for the concept of bovine plasma arylesterase as a lipoprotein

1975 ◽  
Vol 151 (3) ◽  
pp. 625-630 ◽  
Author(s):  
M M Don ◽  
C J Masters ◽  
D J Winzor
Keyword(s):  
Molecular Weight ◽  
High Density Lipoprotein ◽  
Specific Volume ◽  
Neutral Lipids ◽  
Deae Cellulose ◽  
Density Lipoprotein ◽  
Enzymic Activity ◽  
Partial Specific Volume ◽  
Alternative Source ◽  
Bovine Plasma

Purified preparations of bovine plasma arylesterase were obtained by isoelectric focusing of enzyme prepared by (NH4)2SO4 fractionation of plasma and chromatography on DEAE-cellulose and Sephadex G-200. Although the high-density-lipoprotein fraction (HDL2) of serum provides an alternative source of enzyme, the enzymic activity of preparations made from it is much less stable. The purified arylesterase preparation has a molecular weight of 440000 and a partial specific volume of 0.91 ml/g, properties indistinguishable from those of the less highly purified enzyme. Extraction with acetone and ether removes neutral lipids from the enzyme, but the resulting lipid-depleted preparation retains most of the phospholipid present initially. A partial specific volume of 0.81 ml/g and a minimum molecular weight of approx. 100000 were determined for the lipid-depleted preparations of arylesterase. The present results support the concept of bovine plasma arylesterase as a lipoprotein in its own right, rather than as an enzymic polypeptide that is loosely associated with the HDL2 fraction of serum.

PARTIAL SPECIFIC VOLUME MEASUREMENTS BY DIFFERENTIAL SEDIMENTATION

1959 ◽  
Vol 37 (10) ◽  
pp. 1662-1670 ◽  
Author(s):  
W. G. Martin ◽  
C. A. Winkler ◽  
W. H. Cook
Keyword(s):  
Heavy Water ◽  
Isotopic Exchange ◽  
Partial Specific Volume ◽  
Plasma Albumin ◽  
Bovine Plasma ◽  
Water Media ◽  
Volume Measurements ◽  
Differential Sedimentation ◽  
Specific Volumes ◽  
Magnetic Float

The partial specific volumes of several macromolecules were determined from their sedimentation rates in aqueous and heavy water solutions with results comparable to those obtained by conventional methods. Corrections for the changes in weight due to isotopic exchange were obtained from measurements on small quantities of material with a quartz spiral balance. Measurements of the partial specific volumes of bovine plasma albumin, polyvinyl alcohol, glycine, and triglycylglycine in both aqueous and heavy water media, made with a magnetic float apparatus, indicate that isotopic exchange has no significant effect on the macromolecular volume. The sedimentation method is therefore a micro method that does not require a knowledge of the concentration of macromolecules and which is applicable to mixtures that are resolvable during sedimentation.

Bovine Platelet Proteins

1969 ◽  
Vol 21 (03) ◽  
pp. 409-418 ◽  
Author(s):  
S Łopaciuk ◽  
N. O Solum
Keyword(s):  
Bovine Serum ◽  
Protein Composition ◽  
Disc Electrophoresis ◽  
Plasma Fibrinogen ◽  
Plasma Albumin ◽  
Precipitin Line ◽  
Bovine Plasma ◽  
Platelet Proteins

Summary1. The protein composition of bovine platelet extracts has been investigated by immunoelectrophoresis and polyacrylamide disc electrophoresis. The information obtained is discussed as a basis for study on platelet fibrinogen.2. With antiserum to platelet proteins 11 precipitin lines were observed 3 of which corresponded electrophoretically to plasma albumin, fibrinogen and γ-globulin. These lines were not seen using the same antiserum absorbed with bovine plasma. The 8 additional lines were still present indicating that they represented specific platelet components. Antiserum to plasma produced the 3 above-mentioned lines, but no others.3. With antiserum to purified bovine plasma fibrinogen 3 precipitin lines were observed. The fibrinogen line was the dominant one. The 2 additional lines did not disappear by absorption of the antiserum with bovine serum nor by incubation of the extracts with thrombin. The latter treatment totally removed the fibrinogen line.4. A non-fibrinogen precipitin line, observed only with the antiserum to platelet extract and positioned in the β2-globulin region, disappeared by the incubation of platelet extracts with thrombin.

scholarly journals An Endonuclease Associated with Bovine Plasma Albumin Fraction

1972 ◽  
Vol 247 (1) ◽  
pp. 193-198
Author(s):  
Motoaki Anai ◽  
Hiroyuki Haraguchi ◽  
Yasuyuki Takagi
Keyword(s):  
Plasma Albumin ◽  
Albumin Fraction ◽  
Bovine Plasma

The Relation of Free Sulfhydryl Groups to Chromatographic Heterogeneity and Polymerization of Bovine Plasma Albumin*

Biochemistry ◽  
1962 ◽  
Vol 1 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Robert W. Hartley ◽  
Elbert A. Peterson ◽  
Herbert A. Sober
Keyword(s):  
Sulfhydryl Groups ◽  
Plasma Albumin ◽  
Bovine Plasma

CD-resolved secondary structure of bovine plasma albumin in acid-induced isomerization*

2009 ◽  
Vol 22 (3) ◽  
pp. 333-340 ◽  
Author(s):  
SEIICHI ERA ◽  
HIROSHI ASHIDA ◽  
SHUNJI NAGAOKA ◽  
HIROSHI INOUYE ◽  
MASARU SOGAMI
Keyword(s):  
Secondary Structure ◽  
Plasma Albumin ◽  
Bovine Plasma
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