PREPARATIVE PARTITION CHROMATOGRAPHY OF CARBOHYDRATES ON CELITE COLUMNS

1956 ◽  
Vol 34 (10) ◽  
pp. 1365-1371 ◽  
Author(s):  
R. U. Lemieux ◽  
C. T. Bishop ◽  
G. E. Pelletier
Keyword(s):  
Chromatographic Method ◽  
Partition Chromatography ◽  
Analytical Work ◽  
Derivatives Of ◽  
Yield And Purity ◽  
Partially Methylated

Partition on Celite columns, using both elution and extrusion techniques, was found to be a convenient and satisfactory chromatographic method for preparative or analytical work involving carbohydrates and their derivatives. The method is superior to other chromatographic procedures for carbohydrates in resolution of mixtures; cost, ease, and speed of operation; yield and purity of products; and range of applicability. To illustrate these advantages a number of specific examples are given involving partially methylated derivatives of D-glucose and D-xylose and isolation of products from reaction mixtures.

The Influence of Phenothiazine and Some of its Derivatives on the Determination of 5-Hydroxyincioleacetic Acid in Urine

1958 ◽  
Vol 4 (1) ◽  
pp. 66-76 ◽  
Author(s):  
George Ross ◽  
I Bernard Weinstein ◽  
Bernard Kabakow
Keyword(s):  
Urinary Excretion ◽  
Experimental Work ◽  
Chemical Method ◽  
Chromatographic Method ◽  
Metastatic Carcinoid ◽  
Chemical Determination ◽  
Derivatives Of

Abstract The experimental work in this paper demonstrates that derivatives of phenothiazine interfere with the chemical determination of 5-HIAA. This holds true when attempts are made to determine 5-HIAA in the presence of Compazine, Sparine, Phenergan or chlorpromazine, and in the case of the latter substance also when administered to a patient. Thus, it is impossible by the chemical method to determine 5-HIAA in the urine under these circumstances, and results so obtained will be incorrect. However, with the chromatographic method of Curzon (7) no such interference occurs, and, utilizing this technic, it is possible to determine 5-HIAA in the urine of a patient while he is receiving chlorpromazine. In this manner it was shown that the urinary excretion of 5-HIAA in a patient with metastatic carcinoid was not influenced by the administration of chlorpromazine. (See ADDENDUM, p. 76.)

The synthesis, separation, and identification of the methyl ethers of arabinose and their derivatives

1967 ◽  
Vol 45 (3) ◽  
pp. 275-290 ◽  
Author(s):  
S. C. Williams ◽  
J. K. N. Jones
Keyword(s):  
Reducing Sugars ◽  
Reducing Sugar ◽  
The Other ◽  
Partition Chromatography ◽  
Ring Form ◽  
Optical Rotations ◽  
Layer Chromatography ◽  
Derivatives Of ◽  
Spray Reagents

A study has been made of various methods available for the identification and separation of the methyl ethers of arabinose. Gas–liquid partition chromatography has been used to separate the acetylated glycosides and the acetylated alditols of the methyl ethers of arabinose. All of the methyl ethers of arabinopyranose and arabinofuranose have been separated by paper chromatography. Several spray reagents have been used to distinguish between those methyl ethers with similar rates of movement. Thin-layer chromatography has been used to separate the methyl glycosides, acetylated methyl glycosides, and glycitols of the methyl ethers of arabinose, as well as the methyl ethers of the reducing sugar. The optical rotations of the reducing sugars and of the methyl glycosides of the methyl ethers of arabinose provide information about the ring form and, in the case of the glycosides, about the anomer present. The rotations of the acetylated and unacetylated O-methyl arabinitols aid in the determination of the position of the methyl substitutents. In connection with this study, all of the mono-O-methyl and tri-O-methyl, and most of the di-O-methyl ethers of arabinose have been synthesized. New syntheses have been devised for 4-O-methyl and 2,3-di-O-methyl arabinose, and the other sugars have been synthesized by known or partially revised syntheses. During this work, previously unreported derivatives of these sugars have been prepared.

scholarly journals Analyses of Antioxidative Properties of Selected Cyclitols and Their Mixtures with Flavanones and Glutathione

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 158
Author(s):  
Joanna Płonka ◽  
Joanna Szablińska-Piernik ◽  
Bogusław Buszewski ◽  
Irena Baranowska ◽  
Lesław B. Lahuta
Keyword(s):  
Reactive Oxygen Species ◽  
Chromatographic Method ◽  
Antioxidant Properties ◽  
Ros Generation ◽  
Oxygen Species ◽  
Dpph Radical ◽  
Antioxidative Properties ◽  
Spectrophotometric Methods ◽  
First Time ◽  
Derivatives Of

The conditions for determining the antioxidant properties of cyclitols (d-pinitol, l-quebrachitol, myo-, l-chiro-, and d-chiro-inositol), selected flavanones (hesperetin, naringenin, eriodictyol, and liquiritigenin) and glutathione by spectrophotometric methods—CUPRAC and with DPPH radical, and by a chromatographic method DPPH-UHPLC-UV, have been identified. Interactions of the tested compounds and their impact on the ox-red properties were investigated. The RSA (%) of the compounds tested was determined. Very low antioxidative properties of cyclitols, compared with flavanones and glutathione alone, were revealed. However, a significant increase in the determined antioxidative properties of glutathione by methyl-ether derivatives of cyclitols (d-pinitol and l-quebrachitol) was demonstrated for the first time. Thus, cyclitols seem to be a good candidate for creating drugs for the treatment of many diseases associated with reactive oxygen species (ROS) generation.

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