Identification of suitable nontraditional tropical and residual paving materials in relation to the environment

1987 ◽  
Vol 24 (1) ◽  
pp. 58-71 ◽  
Author(s):  
M. D. Gidigasu ◽  
S. P. K. Asante ◽  
E. Dougan
Keyword(s):  
Performance Studies ◽  
Low Cost ◽  
Index Properties ◽  
Moisture Index ◽  
Fines Content ◽  
Geotechnical Characteristics ◽  
Pavement Construction ◽  
The One ◽  
Identification And Characterization

Results of laboratory and field construction and pavement performance studies have revealed that some apparently substandard or borderline—by temperature zone standards—tropical gravel materials can be used successfully for the construction of low-cost pavements in subhumid climatic zones.The geotechnical characteristics of suitable paving materials have been defined from results of studies carried out on satisfactory and poor road sections over a period of 5–6 years.Important correlations were found between the bearing strength (CBR) on the one hand and some moisture index properties as well as the products of the fines content and these index properties on the other.It is shown that the product of the fines content and some moisture index properties form a useful basis for identifying suitable materials for pavement construction in the moist subhumid climatic zone of Ghana.The paper is a contribution to the identification and characterization of nontraditional tropical and residual paving materials in relation to the environment. Key words: pavements, California bearing ratio (CBR), index properties, fines content, subhumid climate.

Identification and characterization of a fibronectin-binding protein from Clostridium difficile

Microbiology ◽  
2003 ◽  
Vol 149 (10) ◽  
pp. 2779-2787 ◽  
Author(s):  
Claire Hennequin ◽  
Claire Janoir ◽  
Marie-Claude Barc ◽  
Anne Collignon ◽  
Tuomo Karjalainen
Keyword(s):  
Clostridium Difficile ◽  
Binding Protein ◽  
Immunoblot Analysis ◽  
Vero Cells ◽  
Cell Fractionation ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding ◽  
The One ◽  
Identification And Characterization

A 68 kDa fibronectin-binding protein (Fbp68) from Clostridium difficile displaying significant homology to several established or putative Fbps from other bacteria was identified. The one-copy gene is highly conserved in C. difficile isolates. Fbp68 was expressed in Escherichia coli in fusion with glutathione S-transferase; the fusion protein and the native Fbp68 were purified. Immunoblot analysis and cell fractionation experiments revealed that Fbp68 is present on the surface of the bacteria. Far-immuno dot-blotting demonstrated that Fbp68 was capable of fixing fibronectin. Indirect immunofluorescence and ELISA were employed to demonstrate that C. difficile could bind both soluble and immobilized fibronectin. With competitive adherence inhibition assays it was shown that antibodies raised against Fbp68 partially inhibited attachment of C. difficile to fibronectin and Vero cells. Furthermore, Vero cells could fix purified membrane-immobilized Fbp68. Thus Fbp68 appears to be one of the several adhesins identified to date in C. difficile.

scholarly journals Identification and characterization of the contact zone between short-tailed shrews (Blarina) in Iowa and Missouri

2011 ◽  
Vol 89 (4) ◽  
pp. 278-288 ◽  
Author(s):  
C.W. Thompson ◽  
R.S. Pfau ◽  
J.R. Choate ◽  
H.H. Genoways ◽  
E.J. Finck
Keyword(s):  
Fragment Length Polymorphism ◽  
Putative Hybrid ◽  
Aflp Analysis ◽  
Contact Zones ◽  
Blarina Brevicauda ◽  
Field Identification ◽  
The One ◽  
Blarina Hylophaga ◽  
Identification And Characterization

Short-tailed shrews (genus Blarina Gray, 1838) are characterized by divergent karyotypes and are genetically distinct. Blarina species are similar morphologically but, in most cases, can be distinguished morphometrically. Blarina distributions tend to be parapatric along well-defined contact zones; however, it has been suggested that the northern short-tailed shrew ( Blarina brevicauda (Say, 1823)) and Elliot’s short-tailed shrew ( Blarina hylophaga Elliot, 1899) occur sympatrically in Iowa and Missouri. To evaluate this possibility, 179 specimens were collected in southwestern Iowa and northwestern Missouri. Karyotypes and total length were used for field identification, and amplified fragment length polymorphism (AFLP) analysis was used to verify field identifications and to investigate the extent of hybridization. One hundred seventy-eight of 179 specimens were identified to species. The one exception had a karyotype of B. brevicauda (2n = 50, FN = 48); however, AFLP analysis indicated that this individual was likely an F1 hybrid. No backcrosses were detected, so it appears that introgression is minimal. The putative hybrid was trapped at a locality with B. brevicauda just north of a locality having only B. hylophaga. No locality contained both species. Therefore, these species are not broadly sympatric as has been suggested, but rather exhibit a distribution similar to the pattern of parapatry seen in most of the contact zones of Blarina.

Identification and characterization of aldose reductase in cultured rat mesangial cells

Diabetes ◽  
1992 ◽  
Vol 41 (9) ◽  
pp. 1165-1171 ◽  
Author(s):  
R. Kikkawa ◽  
K. Umemura ◽  
M. Haneda ◽  
N. Kajiwara ◽  
S. Maeda ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
Mesangial Cells ◽  
Identification And Characterization
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