Lithium preserves F-actin from the disarrangement induced by either DNase I or cytochalasin D

1993 ◽  
Vol 71 (9-10) ◽  
pp. 440-446
Author(s):  
Isabella DalleDonne ◽  
Aldo Milzani ◽  
Umberto Fascio ◽  
Antonia Ratti ◽  
Roberto Colombo
Keyword(s):  
Light Scattering ◽  
Actin Filaments ◽  
Actin Polymerization ◽  
Dnase I ◽  
Cytochalasin D ◽  
Cross Linking ◽  
Lithium Ions ◽  
Scattering Intensity ◽  
Light Scattering Intensity ◽  
Rodlike Particles

Light scattering at 546 nm, which is mainly related to the presence of rodlike particles longer than 50 nm, showed that Li+ accelerates the formation of actin filaments. Intermolecular cross-linking with N,N′-1,4-phenylene-bismaleimide proved that the observed enhancement in the light-scattering intensity is caused by the increase in the concentration of actin oligomers, which gradually elongate to form longer filaments. DNase-I-related F-actin disassembly was reduced in the presence of lithium ions, as demonstrated by fluorimetric and viscometric experiments. Li+–F-actin showed an apparently similar behaviour when exposed to cytochalasin D. We confirm that Li+ acts on actin polymerization by stabilizing actin nuclei and polymers. The stabilization of cytoskeletal polymers really appears as one of the mechanisms by which lithium ions influence some of the cell activities.Key words: lithium, F-actin stability, cytochalasin D, DNase I.

Light Scattering Intensity Fluctuations in Microdroplets Containing Inclusions.

1997 ◽  
Author(s):  
Gorden Videen ◽  
Paul Pellegrino ◽  
Dat Ngo ◽  
John S. Videen ◽  
Ronald G. Pinnick
Keyword(s):  
Light Scattering ◽  
Scattering Intensity ◽  
Light Scattering Intensity ◽  
Intensity Fluctuations

Some Effects on Light Scattering Intensity and Red Blood Cell Size Distribution Histograms Due to Sphering

1988 ◽  
Vol 25 (6) ◽  
pp. 673-679 ◽  
Author(s):  
P C Bartels ◽  
P W Helleman ◽  
A F M Roijers ◽  
J B J Soons
Keyword(s):  
Light Scattering ◽  
Blood Cell ◽  
Size Distribution ◽  
Red Blood Cell ◽  
Signal To Noise Ratio ◽  
Quantitative Information ◽  
Cell Size Distribution ◽  
Scattering Intensity ◽  
Distribution Ranges ◽  
Light Scattering Intensity

At present most haematology blood cell analysers routinely provide red blood cell (RBC) size distribution histograms. Sophisticated improvements of the instruments have re-awakened interest in the study of size histograms. The quantitative information derived from the histograms may be applied more fruitfully if insight is available, with respect to some essential principles of sizing technology and methods for treatment of RBCs before measurement. In this study the consequences of sphering RBCs are investigated in relation to the generation of size distribution histograms by means of methods based on light scattering intensity (LSI). Sphering of RBCs results in considerably narrower histograms than unsphered RBCs. The overall signal to noise ratio increases and there is a broader gap between large platelets and microcytic RBCs. Narrower size distribution ranges will enable closer modes to be separated. Compared to unsphered RBCs, microcytic sphered RBCs yield increased LSI whereas macrocytic sphered RBCs yield decreased LSI.

An enhanced time-of-flight spectrometer that measures aerodynamic size plus light-scattering intensity

1997 ◽  
Vol 28 ◽  
pp. S11-S12 ◽  
Author(s):  
R.L. Holm ◽  
R. Caldow ◽  
P.P. Hairston ◽  
F.R. Quant ◽  
G.J. Sem
Keyword(s):  
Light Scattering ◽  
Time Of Flight ◽  
Scattering Intensity ◽  
Light Scattering Intensity
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