Ion and water distribution in pig lenses incubated at 0 °C to disable ion transport pumps

1991 ◽  
Vol 69 (10-11) ◽  
pp. 742-746 ◽  
Author(s):  
Ivan L. Cameron ◽  
W. Elaine Hardman ◽  
Gary D. Fullerton ◽  
Miklos Kellermayer ◽  
Andrea Ludany ◽  
...  
Keyword(s):  
Water Content ◽  
Active Transport ◽  
Water Distribution ◽  
Content Volume ◽  
Cell Volume Regulation ◽  
Transport Processes ◽  
Water Volume ◽  
Bathing Solution ◽  
Ion Distribution ◽  
Lens Cell

This study was designed to test how extended exposure of lenses to sera with different ionic strengths influences the distribution of ions and water in the lens. Pig lenses were incubated in cold sera (0 °C), which were adjusted to variable concentrations of NaCl, and their K+, Na+, Cl−, and water contents were measured. Incubation at 0 °C inhibits active transport processes and thereby allows equilibration of the mobile ions and water. The hypothesis was that lens water content (volume) would follow the ion-induced protein changes predicted by a model derived from previous osmotic studies on proteins. As expected, exposure of the lens to cold caused a gain of sodium and a partial loss of potassium. However, the potassium concentration in the lens remained several fold higher than that in the bathing solution (about 41 vs. 1.8–4.6 mM/kg H2O), indicating that a portion of the potassium within the cold-exposed lens was not free to diffuse. That the water content of the lens showed a negative rather than a positive relationship with the concentration of NaCl within the lens was explained by the idea that an increase in NaCl within the lens (up to at least 250 mM/kg H2O) causes a decrease in the osmotically unresponsive water volume associated with lens proteins.Key words: pig lens, cell water, Na+, K+, Cl−, osmotic pressure, ion distribution, cell volume regulation, inhibition of active transport.

scholarly journals Osmotic water permeability of Necturus gallbladder epithelium.

1989 ◽  
Vol 93 (4) ◽  
pp. 649-679 ◽  
Author(s):  
C U Cotton ◽  
A M Weinstein ◽  
L Reuss
Keyword(s):  
Water Content ◽  
Time Course ◽  
Basolateral Membrane ◽  
Water Volume ◽  
Hydraulic Permeability ◽  
Bathing Solution ◽  
Gallbladder Epithelium ◽  
Cell Water ◽  
Necturus Gallbladder

An electrophysiological technique that is sensitive to small changes in cell water content and has good temporal resolution was used to determine the hydraulic permeability (Lp) of Necturus gallbladder epithelium. The epithelial cells were loaded with the impermeant cation tetramethylammonium (TMA+) by transient exposure to the pore-forming ionophore nystatin in the presence of bathing solution TMA+. Upon removal of the nystatin a small amount of TMA+ is trapped within the cell. Changes in cell water content result in changes in intracellular TMA+ activity which are measured with intracellular ion-sensitive microelectrodes. We describe a method that allows us to determine the time course for the increase or decrease in the concentration of osmotic solute at the membrane surface, which allows for continuous monitoring of the difference in osmolality across the apical membrane. We also describe a new method for the determination of transepithelial hydraulic permeability (Ltp). Apical and basolateral membrane Lp's were assessed from the initial rates of change in cell water volume in response to anisosmotic mucosal or serosal bathing solutions, respectively. The corresponding values for apical and basolateral membrane Lp's were 0.66 x 10(-3) and 0.38 x 10(-3) cm/s.osmol/kg, respectively. This method underestimates the true Lp values because the nominal osmotic differences (delta II) cannot be imposed instantaneously, and because it is not possible to measure the true initial rate of volume change. A model was developed that allows for the simultaneous determination of both apical and basal membrane Lp's from a unilateral exposure to an anisosmotic bathing solution (mucosal). The estimates of apical and basal Lp with this method were 1.16 x 10(-3) and 0.84 x 10(-3) cm/s.osmol/kg, respectively. The values of Lp for the apical and basal cell membranes are sufficiently large that only a small (less than 3 mosmol/kg) transepithelial difference in osmolality is required to drive the observed rate of spontaneous fluid absorption by the gallbladder. Furthermore, comparison of membrane and transepithelial Lp's suggests that a large fraction of the transepithelial water flow is across the cells rather than across the tight junctions.

Evaluation of Imaging Techniques Applied to Water Management Research in PEMFCs

2009 ◽  
Author(s):  
Michael M. Daino ◽  
Satish G. Kandlikar
Keyword(s):  
Water Management ◽  
Fuel Cells ◽  
Water Content ◽  
Water Distribution ◽  
Imaging Techniques ◽  
Gas Diffusion ◽  
Transport Processes ◽  
Normal Operation ◽  
X Rays ◽  
Efficient Operation

Water management in proton exchange membrane fuel cells (PEMFCs) is critical in efficient operation of fuel cells during normal operation as well as purge and start-up conditions. Insufficient membrane hydration impedes the flow of protons and an overabundance of water obstructs the flow of reactants in the gas diffusion layer (GDL) and in gas distribution channels. These two extremes of water content in PEMFCs significantly reduce performance and efficiency, causing material degradation and potential failure. Visualization and quantitative measurement of water content in PEMFCs lead to greater comprehension of water distribution and transport processes. A wide variety of imaging techniques have been employed in literature to reveal water distribution and transport processes on both macroscale and microscale. The presented techniques utilize visible, infrared, X-rays, fluorescence microscopy, nuclear magnetic resonance (NMR), and neutron radiography to visualize water, measure temperature distributions, and quantify water content. Each imaging technique has intrinsic advantages, disadvantages, and limitations for water detection and will be discussed. A critical evaluation of these techniques and their suitability for visualization of specific components of PEMFC are also discussed.

scholarly journals Assessing the Impact of DMAs and the Use of Boosters on Chlorination in a Water Distribution Network in Greece

Water ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2141
Author(s):  
Stavroula Tsitsifli ◽  
Vasilis Kanakoudis
Keyword(s):  
Water Distribution ◽  
Distribution Network ◽  
Water Distribution Network ◽  
Assessment Process ◽  
Water Volume ◽  
Pressure Management ◽  
Water Age ◽  
Chlorination Process ◽  
Management Measures ◽  
The Impact

Disinfection is one of the most important water treatment processes as it inactivates pathogens providing safe drinking water to the consumers. A fresh-water distribution network is a complex system where constant monitoring of several parameters and related managerial decisions take place in order for the network to operate in the most efficient way. However, there are cases where some of the decisions made to improve the network’s performance level, such as reduction of water losses, may have negative impacts on other significant operational processes such as the disinfection. In particular, the division of a water distribution network into district metered areas (DMAs) and the application of various pressure management measures may impact the effectiveness of the water chlorination process. Two operational measures are assessed in this paper: (a) the use of inline chlorination boosters to achieve more efficient chlorination; and (b) how the DMAs formation impacts the chlorination process. To achieve this, the water distribution network of a Greek town is chosen as a case study where several scenarios are being thoroughly analyzed. The assessment process utilizes the network’s hydraulic simulation model, which is set up in Watergems V8i software, forming the baseline to develop the network’s water quality model. The results proved that inline chlorination boosters ensure a more efficient disinfection, especially at the most remote parts/nodes of the network, compared to conventional chlorination processes (e.g., at the water tanks), achieving 100% safe water volume and consuming almost 50% less chlorine mass. DMAs’ formation results in increased water age values up to 8.27%, especially at the remote parts/nodes of the network and require more time to achieve the necessary minimum effective chlorine concentration of 0.2 mg/L. However, DMAs formation and pressure management measures do not threaten the chlorination’s efficiency. It is important to include water age and residual chlorine as criteria when optimizing water pressure and the division of DMAs.

Comparison of Water Thickness Profiles of Compressed PEMFC GDLs

2011 ◽  
Author(s):  
Pradyumna Challa ◽  
James Hinebaugh ◽  
A. Bazylak
Keyword(s):  
Water Content ◽  
Liquid Water ◽  
Water Distribution ◽  
Polymer Electrolyte Membrane ◽  
Water Injection ◽  
Liquid Water Content ◽  
Gas Diffusion ◽  
Injection Rate ◽  
Water Levels ◽  
Ex Situ

In this paper, through-plane liquid water distribution is analyzed for two polymer electrolyte membrane fuel cell (PEMFC) gas diffusion layers (GDLs). The experiments were conducted in an ex situ flow field apparatus with 1 mm square channels at two distinct flow rates to mimic water production rates of 0.2 and 1.5 A/cm2 in a PEMFC. Synchrotron radiography, which involves high intensity monochromatic X-ray beams, was used to obtain images with a spatial and temporal resolution of 20–25 μm and 0.9 s, respectively. Freudenberg H2315 I6 exhibited significantly higher amounts of water than Toray TGP-H-090 at the instance of breakthrough, where breakthrough describes the event in which liquid water reaches the flow fields. While Freudenberg H2315 I6 exhibited a significant overall decrease in liquid water content throughout the GDL shortly after breakthrough, Toray TGP-H-090 appeared to retain breakthrough water-levels post-breakthrough. It was also observed that the amount of liquid water content in Toray TGP-H-090 (10%.wt PTFE) decreased significantly when the liquid water injection rate increased from 1 μL/min to 8 μL/min.

scholarly journals The Transport of Salt and Water across Isolated Rat Ileum

1967 ◽  
Vol 50 (3) ◽  
pp. 695-727 ◽  
Author(s):  
T. W. Clarkson
Keyword(s):  
Active Transport ◽  
Transport Processes ◽  
Irreversible Processes ◽  
Pressure Gradients ◽  
Ion Flow ◽  
Chemical Gradients ◽  
In Series ◽  
Frictional Coefficients ◽  
The Relationship ◽  
Active Channel

The flows of sodium, potassium, and chloride under electrical and chemical gradients and of salt and water in the presence of osmotic pressure gradients are described by phenomenological equations based on the thermodynamics of irreversible processes. The aim was to give the simplest possible description, that is to postulate the least number of active transport processes and the least number of separate pathways across the intestine. On this basis, the results were consistent with the following picture of the intestine: Two channels exist across this tissue, one allowing only passive transport of ions and the other only active. In the passive channel, the predominant resistance to ion flow is friction with the water in the channel. The electroosmotic flow indicates that the passive channel is lined with negative fixed charged groups having a surface charge density of 3000 esu cm-2. The values of the ion-water frictional coefficients, and the relationship between ionic concentrations and flows indicate that the passive channel is extracellular. The active channel behaves as two membranes in series, the first membrane being semipermeable but allowing active transport of sodium, and the second membrane being similar to the passive channel. Friction with the ions in the second "membrane" is the predominant resistance to water flow.

Experimental Spinal Cord Injury: Spatiotemporal Characterization of Elemental Concentrations and Water Contents in Axons and Neuroglia

1999 ◽  
Vol 82 (5) ◽  
pp. 2143-2153 ◽  
Author(s):  
Richard M. LoPachin ◽  
Christopher L. Gaughan ◽  
Ellen J. Lehning ◽  
Yoshiro Kaneko ◽  
Thomas M. Kelly ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Water Content ◽  
Severely Injured ◽  
Channel Blockade ◽  
Compression Injury ◽  
Ion Distribution ◽  
Elemental Concentrations ◽  
Cord Injury ◽  
Spinal Segments

To examine the role of axonal ion deregulation in acute spinal cord injury (SCI), white matter strips from guinea pig spinal cord were incubated in vitro and were subjected to graded focal compression injury. At several postinjury times, spinal segments were removed from incubation and rapidly frozen. X-ray microanalysis was used to measure percent water and dry weight elemental concentrations (mmol/kg) of Na, P, Cl, K, Ca, and Mg in selected morphological compartments of myelinated axons and neuroglia from spinal cord cryosections. As an index of axon function, compound action potentials (CAP) were measured before compression and at several times thereafter. Axons and mitochondria in epicenter of severely compressed spinal segments exhibited early (5 min) increases in mean Na and decreases in K and Mg concentrations. These elemental changes were correlated to a significant reduction in CAP amplitude. At later postcompression times (15 and 60 min), elemental changes progressed and were accompanied by alterations in compartmental water content and increases in mean Ca. Swollen axons were evident at all postinjury times and were characterized by marked element and water deregulation. Neuroglia and myelin in severely injured epicenter also exhibited significant disruptions. In shoulder areas (adjacent to epicenter) of severely injured spinal strips, axons and mitochondria exhibited modest increases in mean Na in conjunction with decreases in K, Mg, and water content. Following moderate compression injury to spinal strips, epicenter axons exhibited early (10 min postinjury) element and water deregulation that eventually recovered to near control values (60 min postinjury). Na+ channel blockade by tetrodotoxin (TTX, 1 μM) perfusion initiated 5 min after severe crush diminished both K loss and the accumulation of Na, Cl, and Ca in epicenter axons and neuroglia, whereas in shoulder regions TTX perfusion completely prevented subcellular elemental deregulation. TTX perfusion also reduced Na entry in swollen axons but did not affect K loss or Ca gain. Thus graded compression injury of spinal cord produced subcellular elemental deregulation in axons and neuroglia that correlated with the onset of impaired electrophysiological function and neuropathological alterations. This suggests that the mechanism of acute SCI-induced structural and functional deficits are mediated by disruption of subcellular ion distribution. The ability of TTX to reduce elemental deregulation in compression-injured axons and neuroglia implicates a significant pathophysiological role for Na+ influx in SCI and suggests Na+ channel blockade as a pharmacotherapeutic strategy.

Development of a soil water dispersion index (SOWADIN) for testing the effectiveness of soil-wetting agents

Soil Research ◽  
1989 ◽  
Vol 27 (1) ◽  
pp. 17 ◽  
Author(s):  
Y Sawada ◽  
LAG Aylmore ◽  
JM Hainsworth
Keyword(s):  
Water Content ◽  
Soil Water ◽  
Water Distribution ◽  
Soil Column ◽  
Water Repellent ◽  
Computer Assisted ◽  
Dispersion Index ◽  
Soil Columns ◽  
Water Dispersion ◽  
Soil Wetting

Computer-assisted tomography (CAT) applied to gamma-ray attenuation measurements has been used to develop an index termed the soil water dispersion index (SOWADIN), which describes quantitatively the amount and distribution of water in soil columns. The index, which is determined by classifying pixels in a scanned slice into three categories according to their attenuation coefficients, contains two numerical values. The first value corresponds to the water content of the scanned slice and the second value is a measure of the dispersion of the water throughout the slice. Artificially wetted zones were created in soil columns to give one-third of the scanned layer wetted with various patterns of wetted-area distribution. The SOWADIN values obtained accurately reflected the differences in water distribution associated with the different patterns. Application of SOWADIN to columns of a water-repellent sand before and after treatment with a soil-wetting agent clearly illustrates both the increase in water content and improvement in water distribution in the soil column following treatment.

scholarly journals CARACTERIZAÇÃO DA DISTRIBUIÇÃO DA ÁGUA NO SOLO PARA IRRIGAÇÃO POR GOTEJAMENTO

Irriga ◽  
2018 ◽  
Vol 14 (4) ◽  
pp. 564-577 ◽  
Author(s):  
Leonardo Do Nascimento Lopes ◽  
Elton Martins ◽  
Bruno De Lima Santoro ◽  
Claudinei Fonseca Souza
Keyword(s):  
Water Content ◽  
Time Domain ◽  
Water Distribution ◽  
Water Storage ◽  
Time Domain Reflectometry ◽  
Wetting Front ◽  
Applied Water ◽  
Flow Rates ◽  
Abstract Knowledge ◽  
The Time Domain

CARACTERIZAÇÃO DA DISTRIBUIÇÃO DA ÁGUA NO SOLO PARA IRRIGAÇÃO POR GOTEJAMENTO  Leonardo do Nascimento Lopes1; Elton Martins2; Bruno de Lima Santoro2; Claudinei Fonseca Souza31Universidade de Taubaté, Unitau, Taubaté, SP,  [email protected] Engenharia Civil e Ambiental, Universidade de Taubaté, Taubaté, Unitau, SP 3Departamento de Recursos Naturais e Proteção Ambiental, Universidade Federal São Carlos, São Carlos, SP  1 RESUMO O conhecimento da distribuição da água no solo é de grande importância para a agricultura, uma vez que a água é um dos fatores que mais influenciam o rendimento das culturas. Existem muitas técnicas utilizadas para o monitoramento do conteúdo de água do solo, a reflectometria domínio do tempo (TDR) tem sido difundida entre os pesquisadores por apresentar várias vantagens, entre as quais a determinação em tempo real e a possibilidade de leituras automatizadas. O principal objetivo desta pesquisa foi avaliar a distribuição da água no perfil de um Latossolo Vermelho-Amarelo. Sondas de Reflectometria no domínio do Tempo (TDR) foram utilizadas para monitorar a distribuição de água no solo aplicada através de gotejadores de fluxo constante nas taxas de 2, 4 e 8 Lh-1. Considerando os resultados de diferentes perfis, observa-se um maior armazenamento da água próximo do gotejador diminuindo progressivamente para frente de molhamento. Aproximadamente, um terço da água aplicada (33%) foi armazenado na primeira camada (0-0,10 m) para todos os ensaios. Comparando diferentes taxas de aplicação, observa-se maior armazenamento de água para o gotejador de 8L h-1, com 30, 33 e 34% de água aplicada acumulada na primeira camada (0-0.10 m) para gotejadores de 2, 4 e 8L h-1, respectivamente. Os resultados sugerem que, com base no volume e frequência utilizada neste experimento, seria vantajoso aplicar pequenas quantidades de água em intervalos mais frequentes para reduzir perdas por percolação. UNITERMOS: TDR, conteúdo de água, bulbo molhado  LOPES, L. N.; MARTINS, E.; SANTORO, B. L.; SOUZA, C. F.WATER DISTRIBUTION CHARACTERIZATION IN SOIL FOR DRIP IRRIGATION   2 ABSTRACT Knowledge of water distribution in soil is of great importance to agriculture, since water is one of the factors that most influence the yield of crops. There are many techniques used to monitor soil water content. The time domain reflectometry (TDR) has been widespread among researchers because it presents several advantages, among which the determination in real time and possibility of automated readings. The main goal of this research was to evaluatethe water distribution in a profile of Red-Yellow Oxisol. Time domain reflectometry (TDR) probes were used to monitor the water distribution from drippers discharging at constant flow rates of 2, 4 and 8 Lh-1 in soil. Considering results from different profiles, we observed greater water storage near the dripper decreasing gradually towards the wetting front. About one third of the applied water (33%) was stored in the first layer (0-0.10 m) for all experiments. Comparing different dripper flow rates, we observed higher water storage for 8 L h-1, with 30, 33 and 34% of applied water accumulating in the first layer (0-0.10m) for dripper flow rates of 2, 4 and 8 L h-1, respectively. The results suggest that based on the volume and frequency used in this experiment, it would be advantageous to apply small amounts of water at more frequent intervals to reduce deep percolation losses of applied water. KEYWORDS: TDR, water content, wetted soil volume

scholarly journals Monitoring of Soil Water Content in Maize Rotated with Pigeonpea Fallows in South Africa

Water ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2761
Author(s):  
Misheck Musokwa ◽  
Paramu L. Mafongoya ◽  
Paxie W. Chirwa
Keyword(s):  
South Africa ◽  
Water Content ◽  
Soil Water ◽  
Water Distribution ◽  
Smallholder Farmers ◽  
Block Design ◽  
Maize Production ◽  
Soil Water Tension ◽  
Continuous Maize ◽  
Water Tension

Maize production under smallholder systems in South Africa (RSA) depends on rainfall. Incidences of dry spells throughout the growing season have affected maize yields negatively. The study examined water distribution and water use efficiency (WUE) of maize rotated with two-year pigeonpea fallows as compared to continuous maize without fertilizer. A randomized complete block design, replicated three times, was used with four treatments, which included continuous unfertilized maize, natural fallow-maize, pigeonpea + grass-pigeonpea-maize, and two-year pigeonpea fallow-maize. Soil water mark sensors were installed 0.2; 0.5; and 1.2 m on each plot to monitor soil water tension (kPa). Soil samples were analyzed using pressure plates to determine water retention curves which were used to convert soil water tension to volumetric water content. Maize rotated with two-year pigeonpea fallows had higher dry matter yield (11,661 kg ha−1) and WUE (20.78 kg mm−1) than continuous maize (5314 kg ha−1 and 9.48 kg mm−1). In this era of water scarcity and drought incidences caused by climate change, maize rotated with pigeonpea fallows is recommended among smallholder farmers in RSA because of its higher WUE, hence food security will be guaranteed.

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