Levels of sulfogalactosylglycerolipid in capacitated motile and immotile mouse spermatozoa

1990 ◽  
Vol 68 (2) ◽  
pp. 528-535 ◽  
Author(s):  
Nongnuj Tanphaichitr ◽  
Jacqueline Smith ◽  
Morris Kates
Keyword(s):  
Sperm Motility ◽  
Culture Medium ◽  
Mature Male ◽  
Percoll Gradient ◽  
Direct Relation ◽  
Sperm Capacitation ◽  
Epididymal Sperm ◽  
Pellet Fraction ◽  
Mouse Sperm ◽  
Time Point

The purpose of this study was to determine whether sulfogalactosylglycerolipid (SGG) was desulfated during mouse sperm capacitation. Levels of [35S]SGG were determined in freshly retrieved caudal epididymal sperm, motile capacitated sperm, and immotile sperm, after feeding mature male mice with [35S]sulfate-laced chow for 32 days. Caudal epididymal sperm and coisolated epididymal cells were separated into pellet and interphase fractions by centrifugation through a two-step Percoll gradient (45 and 90%). Upon resuspension in Krebs–Ringer bicarbonate medium supplemented with 0.4% bovine serum albumin, the Percoll-gradient pellet fraction consisted mainly of motile capacitated sperm, whereas the interphase fraction comprised largely immotile sperm and fragmented epididymal epithelial cells. The level of [35S]SGG in the Percoll-gradient-pelleted sperm appeared to be much higher than that in the Percoll-gradient interphase sperm. Percoll-gradient-pelleted sperm were further incubated in the culture medium for 2 h. The level of [35S]SGG showed little or no change after 1 h, but was reduced appreciably after 2 h. At this time point, sperm motility was also decreased. Reduction of sperm SGG is correlated with sperm immotility and (or) senescence and may have no direct relation to the capacitation process.Key words: sulfogalactosylglycerolipid, sperm motility, sperm capacitation.

Mouse Sperm Association During Epididymal Transit

2000 ◽  
Vol 6 (S2) ◽  
pp. 968-969
Author(s):  
M. Monclus ◽  
M. H. Burgos ◽  
M.W. Fornés
Keyword(s):  
Sperm Motility ◽  
Culture Medium ◽  
Cell Interaction ◽  
Sperm Head ◽  
Mouse Sperm ◽  
Epididymal Fluid ◽  
Epididymal Duct ◽  
Cell Cell

Head-to-head sperm association was described in some species as a normal phenomenon during epididyma transit (Phillips and Bedford, 1987). Recently, a new sperm head association (SHA) was characterized in the rat (Fornés & Burgos 1990; 1994).At this moment, the study of SHA was extended to mouse sperm. SHA is a type of cell-cell interaction clearly observed when an a drop of epididymal fluid, obtained by a puncture of epididymal duct, is placed in a culture medium (HMB media: Modified Ringers medium).This preparation can be observed under contrast phase microscope (optiphot-2, Nikkon) with or without fixative (2% paraformaldehyde and 2% glutaraldehyde in 0.01 M PBS buffer, pH 7,2). The mass of spermatozoa begin to move but not as a single sperm. They move as a clump of sperm and without fixative the sperm motility alone disaggregate the SHA.

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

Measurement of epididymal sperm motility as a test variable in the rat

1986 ◽  
Vol 36 (1) ◽  
pp. 317-324 ◽  
Author(s):  
Ralph E. Linder ◽  
Lillian F. Strader ◽  
W. Keith McElroy
Keyword(s):  
Sperm Motility ◽  
Epididymal Sperm ◽  
Test Variable

scholarly journals Sperm motility assessment of epididymal sperm from post mortem goat testicles held at 5°C

2017 ◽  
Vol 4 (2) ◽  
pp. 16
Author(s):  
Angelica C. Bumanlag ◽  
Hannah Lei M. Harada ◽  
Cynthia C. Divina ◽  
Marlon B. Ocampo ◽  
Lerma C. Ocampo
Keyword(s):  
Sperm Motility ◽  
Storage Time ◽  
Genetic Material ◽  
Holding Time ◽  
Considerable Proportion ◽  
Prolonged Storage ◽  
Post Mortem ◽  
Epididymal Sperm ◽  
Yield Quality ◽  
Slow Moving

Appropriate holding conditions for post mortem testicles of goat to yield quality epididymal sperm (ES) as a source of genetic material for cryobanking and fertilization studies are lacking. In this study, the effect of storage time on the motility of ES from post mortem testicles maintained at 5°C was evaluated. In the laboratory, the cauda epididymides were excised from the testicles after 4 hr (G-1) and 24 hr (G-2) of holding time before collecting the sperm in a Tris-citrate buffered solution and evaluated using a CASA. Sperm motility profiling revealed a subpopulation of static, slow, motile and progressive ES. The proportion of static sperm in the control (16.97±6.21) and G-1 (21.53±5.60) were lower significantly than G-2 (36.13±5.05). The proportion of slow moving sperm was lower significantly than G-1 (23.31±3.57) and G-2 (25.45±3.32). The proportion of motile and progressive motile sperm decreases significantly (P<0.05) as the holding time increases at 78.46±4.64% (G-1) to 63.85±4.06 (G-2) and 45.53±8.89 (G-1) to 25.46±8.42 (G-2), respectively. The results showed that prolonged storage of post mortem testicles at 5°C could result to a reduced percentage of motile and progressively motile ES. Nevertheless, this considerable proportion of ES remained useful both for cryobanking and fertilization studies.

Spermatotoxic effects of carbaryl in rats

1996 ◽  
Vol 15 (9) ◽  
pp. 736-738 ◽  
Author(s):  
N. Pant ◽  
R. Shankar ◽  
SP Srivastava
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Young Male ◽  
Male Rats ◽  
Epididymal Sperm ◽  
Abnormal Morphology ◽  
Age Dependent ◽  
Effect Level

The spermatotoxic effect of carbaryl in adult and young male rats has been examined. Carbaryl 50 and 100 mg/kg b.wt. Male fed 5 d/week for 60 days, caused dose and age- dependent decline in epididymal sperm count and sperm motility, an increase in sperm with abnormal morphology. The dose of 25 mg/kg/d was a 'No observed effect level' for the indices studied. Young animals in comparison to adults exhibited pronounced spermatotoxic effects.

scholarly journals Involvement of a Na+/HCO Cotransporter in Mouse Sperm Capacitation

2002 ◽  
Vol 278 (9) ◽  
pp. 7001-7009 ◽  
Author(s):  
Ignacio A. Demarco ◽  
Felipe Espinosa ◽  
Jennifer Edwards ◽  
Julian Sosnik ◽  
José Luis de la Vega-Beltrán ◽  
...  
Keyword(s):  
Sperm Capacitation ◽  
Mouse Sperm
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