scholarly journals Sperm motility assessment of epididymal sperm from post mortem goat testicles held at 5°C

2017 ◽  
Vol 4 (2) ◽  
pp. 16
Author(s):  
Angelica C. Bumanlag ◽  
Hannah Lei M. Harada ◽  
Cynthia C. Divina ◽  
Marlon B. Ocampo ◽  
Lerma C. Ocampo
Keyword(s):  
Sperm Motility ◽  
Storage Time ◽  
Genetic Material ◽  
Holding Time ◽  
Considerable Proportion ◽  
Prolonged Storage ◽  
Post Mortem ◽  
Epididymal Sperm ◽  
Yield Quality ◽  
Slow Moving

Appropriate holding conditions for post mortem testicles of goat to yield quality epididymal sperm (ES) as a source of genetic material for cryobanking and fertilization studies are lacking. In this study, the effect of storage time on the motility of ES from post mortem testicles maintained at 5°C was evaluated. In the laboratory, the cauda epididymides were excised from the testicles after 4 hr (G-1) and 24 hr (G-2) of holding time before collecting the sperm in a Tris-citrate buffered solution and evaluated using a CASA. Sperm motility profiling revealed a subpopulation of static, slow, motile and progressive ES. The proportion of static sperm in the control (16.97±6.21) and G-1 (21.53±5.60) were lower significantly than G-2 (36.13±5.05). The proportion of slow moving sperm was lower significantly than G-1 (23.31±3.57) and G-2 (25.45±3.32). The proportion of motile and progressive motile sperm decreases significantly (P<0.05) as the holding time increases at 78.46±4.64% (G-1) to 63.85±4.06 (G-2) and 45.53±8.89 (G-1) to 25.46±8.42 (G-2), respectively. The results showed that prolonged storage of post mortem testicles at 5°C could result to a reduced percentage of motile and progressively motile ES. Nevertheless, this considerable proportion of ES remained useful both for cryobanking and fertilization studies.

scholarly journals Effect of Cold Storage on Livability and Progressive Motility of Cauda Epididymal Spermatozoa of Red Sokoto Buck Recovered Post Mortem

2021 ◽  
Author(s):  
Faith Chinonye Eke ◽  
Terzungwe Ahemen
Keyword(s):  
Sperm Motility ◽  
Cold Storage ◽  
Standard Error ◽  
Storage Time ◽  
Post Mortem ◽  
Time Processing ◽  
Progressive Motility ◽  
Epididymal Spermatozoa

Post mortem spermatozoa recovery is an important tool for harvesting germplasm. The study aimed to determine the effect of cold storage time at 4°C for 48 and 72 hours on the quality of sperm of Red Sokoto buck caudal epididymaly spermatozoa in terms of motility and livability recovered postmortem. Fifteen testicles with attached epididymis were collected and processed at 0, 48, and 72 hours of storage. The samples were divided into 3 groups according to the time processing after collection ; 0 hour, 48 hours and 72 hours, with a total of 5 samples per group. Each storage group motility and livability was evaluated, mean and the standard error of their mean was recorded. Mean percent sperm motility declined significantly ( p< 0.05) as postmortem storage time increased ( motility: 79.00+/- 2.92%, 40.00+/- 4.18, 37.00+/- 3.39) .There was no significant (p> 0.05) decrease in livability from the 0 hour storage to the 48 hour and 72 hours storage ( 75.60+/- 4.40, 73.60+/- 3.84, 68.20+/- 2.51). The current study has shown that viable spermatozoa can be recovered after 48 hours of storage at 4˚C of the cauda epididymides Red Sokoto buck.

scholarly journals The accuracy of gypsum casts obtained from the disinfected extended-pour alginate impressions through prolonged storage times

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Rania A. Sharif ◽  
Khalid M. Abdelaziz ◽  
Najla M. Alshahrani ◽  
Fatimah S. Almutairi ◽  
Mohrah A. Alaseri ◽  
...  
Keyword(s):  
Dimensional Stability ◽  
Storage Time ◽  
Prolonged Storage ◽  
Time Intervals ◽  
Superior Surface ◽  
Stability Data ◽  
Surface Detail ◽  
Storage Times ◽  
One Way Anova ◽  
Chemical Disinfection

Abstract Background Manufacturers of the extended-pour alginates claimed their dimensional stability through prolonged storage. No data confirmed the ability of these materials to maintain their dimensions and the reproduced oral details following their chemical disinfection. Therefore, this study evaluated the dimensional stability and surface detail reproduction of gypsum casts obtained from disinfected extended-pour alginate impressions through different storage time intervals. Methods Two hundred and forty three hydrocolloid impressions were made from one conventional (Tropicalgin) and two extended-pour (Hydrogum 5 and Chromaprint premium) alginates. These impressions were subjected to none, spray and immersion disinfection before their storage in 100% humidity for 0, 72 and 120 h. The dimensional stability and the surface detail reproduction were indirectly evaluated under low angle illumination on the resulted gypsum casts. At α = 0.05, the parametric dimensional stability data were analyzed using One-Way ANOVA and Tukey’s comparisons, while the nonparametric detail reproduction data were analyzed using KrusKal Wallis and Mann–Whitney's tests. Results All gypsum casts exhibited a degree of expansion; however, the recorded expansion values did not differ between test categories (P > 0.05). Generally, casts obtained from spray-disinfected impressions showed lower detail accuracy (P < 0.05). Immersion-disinfected extended-pour alginates produced casts with better detail accuracy following 120 h of storage (P < 0.05). Conclusion All alginates materials offer comparable cast dimensions under different testing circumstances. Extended-pour alginates offer casts with superior surface details following their immersion disinfection and 120 h of storage. Spray-disinfection using 5.25% sodium hypochlorite affects the surface details of casts obtained from conventional and extended-pour alginates adversely.

Fresh Osteochondral Allograft Transplantation: Is Graft Storage Time Associated With Clinical Outcomes and Graft Survivorship?

2017 ◽  
Vol 45 (10) ◽  
pp. 2260-2266 ◽  
Author(s):  
Kenneth J. Schmidt ◽  
Luís E. Tírico ◽  
Julie C. McCauley ◽  
William D. Bugbee
Keyword(s):  
Clinical Outcomes ◽  
Storage Time ◽  
Osteochondral Allograft ◽  
Prolonged Storage ◽  
Graft Size ◽  
Early Release ◽  
Fresh Osteochondral Allograft ◽  
Release Group ◽  
The Mean

Background: Regulatory concerns and the popularity of fresh osteochondral allograft (OCA) transplantation have led to a need for prolonged viable storage of osteochondral grafts. Tissue culture media allow a longer storage time but lead to chondrocyte death within the tissue. The long-term clinical consequence of prolonged storage is unknown. Hypothesis: Patients transplanted with OCAs with a shorter storage time would have lower failure rates and better clinical outcomes than those transplanted with OCAs with prolonged storage. Study Design: Cohort study; Level of evidence, 3. Methods: A matched-pair study was performed of 75 patients who received early release grafts (mean storage, 6.3 days [range, 1-14 days]) between 1997 and 2002, matched 1:1 by age, diagnosis, and graft size, with 75 patients who received late release grafts (mean storage time, 20.0 days [range, 16-28 days]) from 2002 to 2008. The mean age was 33.5 years, and the median graft size was 6.3 cm2. All patients had a minimum 2-year follow-up. Evaluations included pain, satisfaction, function, failures, and reoperations. Outcome measures included the modified Merle d’Aubigné-Postel (18-point) scale, International Knee Documentation Committee (IKDC) form, and Knee Society function (KS-F) scale. Clinical failure was defined as revision OCA transplantation or conversion to arthroplasty. Results: Among patients with grafts remaining in situ, the mean follow-up was 11.9 years (range, 2.0-16.8 years) and 7.8 years (range, 2.3-11.1 years) for the early and late release groups, respectively. OCA failure occurred in 25.3% (19/75) of patients in the early release group and 12.0% (9/75) of patients in the late release group ( P = .036). The median time to failure was 3.5 years (range, 1.7-13.8 years) and 2.7 years (range, 0.3-11.1 years) for the early and late release groups, respectively. The 5-year survivorship of OCAs was 85% for the early release group and 90% for the late release group ( P = .321). No differences in postoperative pain and function were noted between the groups. Ninety-one percent of the early release group and 93% of the late release group reported satisfaction with OCA results. Conclusion: The transplantation of OCA tissue with prolonged storage is safe and effective for large osteochondral lesions of the knee and has similar clinical outcomes and satisfaction to the transplantation of early release grafts.

Measurement of epididymal sperm motility as a test variable in the rat

1986 ◽  
Vol 36 (1) ◽  
pp. 317-324 ◽  
Author(s):  
Ralph E. Linder ◽  
Lillian F. Strader ◽  
W. Keith McElroy
Keyword(s):  
Sperm Motility ◽  
Epididymal Sperm ◽  
Test Variable

Characteristics of diluted-stored and post-thawed semen of Hutsul stallions

2021 ◽  
Keyword(s):  
Sperm Motility ◽  
Disease Transmission ◽  
Survival Rates ◽  
Genetic Material ◽  
Sperm Bank ◽  
Resource Protection ◽  
Frozen Semen ◽  
Genetic Reserve ◽  
Basic Parameters ◽  
Risk Of Disease

Abstract The use of frozen semen lowers the risk of disease transmission, eliminates geographical limitations and supports the implementation of genetic resource protection programs. However, due to the very rare use of frozen semen from Hutsul stallions, their genetic material is not secured in sperm banks, and very little information is available about their semen, including its suitability for cryopreservation, and sperm survival rates after thawing. The aim of this study was to analyse basic parameters such as sperm motility, vitality and morphology in diluted-stored and post-thawed Hutsul semen, using a CASA system. There were no differences in sperm motility (P = 0.3372) or morphology between the groups, although the progressive motility was higher in thawed semen (P = 0.0151), while the sperm vitality was higher in diluted-stored semen (P = 0.00517). This study demonstrates that semen from Hutsul horses is suitable for cryopreservation, thus supporting the creation of a sperm bank as a genetic reserve for representatives of this breed.

Effect of Storage Time on the Physicochemical and Sensory Properties of Man-tou (Chinese Steamed Bread)

2007 ◽  
Vol 3 (3) ◽  
Author(s):  
Kun Sha ◽  
Ping Qian ◽  
Li-Jun Wang ◽  
Zhan-hui Lu ◽  
Li-Te Li
Keyword(s):  
Water Retention ◽  
Storage Time ◽  
Soluble Starch ◽  
Prolonged Storage ◽  
Water Retention Capacity ◽  
Study Quality ◽  
Total Water ◽  
Retention Capacity ◽  
Steamed Bread

In this study, quality of Man-tou, the Chinese traditional steamed bread during storage was studied. Values for water retention capacity, total water solubles, soluble starch, and soluble amylose and amylopectin of Man-tou decreased with storage time. Results showed that hardness, chewiness and gumminess of Man-tou increased, while, springiness cohesiveness and resilience decreased along with prolonged storage time (p<0.05). Sensory evaluation results showed that all sensory rating of Man-tou, including softness, stickiness, cohesiveness, elasticity, taste and total score, dropped drastically within 24 h of storage time (p<0.05). Differential Scanning Calorimeter (DSC) was used to determine crystalline structure of amylopectin in Man-tou, and the value for enthalpy of crystallization was found increased with storage time. Amylopectin crystallization was significantly associated to Man-tou firming (p<0.01).

Spermatotoxic effects of carbaryl in rats

1996 ◽  
Vol 15 (9) ◽  
pp. 736-738 ◽  
Author(s):  
N. Pant ◽  
R. Shankar ◽  
SP Srivastava
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Young Male ◽  
Male Rats ◽  
Epididymal Sperm ◽  
Abnormal Morphology ◽  
Age Dependent ◽  
Effect Level

The spermatotoxic effect of carbaryl in adult and young male rats has been examined. Carbaryl 50 and 100 mg/kg b.wt. Male fed 5 d/week for 60 days, caused dose and age- dependent decline in epididymal sperm count and sperm motility, an increase in sperm with abnormal morphology. The dose of 25 mg/kg/d was a 'No observed effect level' for the indices studied. Young animals in comparison to adults exhibited pronounced spermatotoxic effects.

Some Osmotic Measurements of the Molecular Weight of a High Molecular-Weight Fraction of Rubber Hydrocarbon

1955 ◽  
Vol 28 (2) ◽  
pp. 504-507
Author(s):  
G. W. Drake
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Average Molecular Weight ◽  
Weight Fraction ◽  
Considerable Proportion ◽  
Prolonged Storage ◽  
High Molecular Weight Fraction ◽  
The United Kingdom ◽  
Molecular Weight Fractions ◽  
Temperate Climates

Abstract Fractionation of the rubber hydrocarbon in temperate climates has usually resulted in high molecular-weight fractions, with a molecular weight of the order of one million. Bloomfield has shown that fresh latex contains a considerable proportion of hydrocarbon having an intrinsic viscosity (η) of 10 or over and, therefore, a molecular weight of well over 106. The fractionation technique used by Bloomfield in Malaya has now been applied by the writer to smoked sheet and to F rubber, working in the United Kingdom. No very high molecular-weight fractions were found in the smoked sheet, but the F rubber yielded a fraction of (η)=7.3 and a number average molecular weight 6×106, determined osmometrically. The average molecular weight of natural rubber when freshly prepared is probably well over a million, and includes a substantial portion having a molecular weight of several millions. By the time smoked sheet has reached temperate climates, the high molecular-weight portion has probably been converted to gel. F rubber, presumably because of its different method of preparation, retains the major part of its high molecular-weight material during prolonged storage.

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