Structure of the lipopolysaccharide O-chain of Yersinia enterocolitica serotype O:5,27

1987 ◽  
Vol 65 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Malcolm B. Perry ◽  
Leann L. MacLean
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Yersinia Enterocolitica ◽  
Lipid A ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Specific Optical Rotation ◽  
Core Oligosaccharide ◽  
Magnetic Resonance Studies ◽  
Serotype O

The cellular lipopolysaccharide produced by Yersinia enterocolitica serotype O:5,27 was of the S-type and composed of an antigenic O-chain polysaccharide linked through a core oligosaccharide region, which in turn was linked through 3-deoxy-D-manno-octulonosyl units to a lipid A moiety. The O-chain polysaccharide was composed of equal molar amounts of L-rhamnose and D-xylulose. By partial hydrolysis, periodate oxidation, methylation, specific optical rotation, and 13C and 1H nuclear magnetic resonance studies, the structure of the O-chain was established as being a linear backbone of alternating 1,3-linked α-L-rhamnopyranosyl and β-L-rhamnopyranosyl units, to which 2,2-linked β-D-threo-pent-2-ulofuranoside (D-xylulofuranoside) units were present on every L-rhamnopyranosyl residue, as shown below.[Formula: see text]

Structure of the specific capsular polysaccharide of Streptococcus pneumoniae type 23F (American type 23)

1988 ◽  
Vol 66 (7) ◽  
pp. 758-771 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
High Resolution ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Composition Analysis ◽  
Part D ◽  
Magnetic Resonance Studies

The specific capsular polysaccharide of Streptococcus pneumoniae serotype 23F (American type 23) is composed of a repeating tetrasaccharide unit containing D-glucose (one part), D-galactose (one part), L-rhamnose (two parts), glycerol (one part), and phosphate (one part). By composition analysis, optical rotation, partial hydrolysis, periodate oxidation, methylation, and high-resolution 1H and 13C nuclear magnetic resonance studies, the elucidated unambiguous structure was in agreement with our earlier proposal but is at variance with structures proposed later by other authors. The structure of the type 23F pneumococcal polysaccharide is[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 9V

1981 ◽  
Vol 59 (7) ◽  
pp. 524-533 ◽  
Author(s):  
Malcolm B. Perry ◽  
Virginia Daoust ◽  
Dennis J. Carlo
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Part D ◽  
Magnetic Resonance Studies

The specific capsular polysaccharide produced by Streptococcus pneumoniae type 9V (American type 68) is composed of D-glucuronic acid (1 part), D-galactose (1 part), 2-acetamido-2-deoxy-D-mannose (1 part), D-glucose (2 parts), and O-acetyl (1.6 parts). Methylation, periodate oxidation, optical rotation, and nuclear magnetic resonance studies, and partial hydrolysis showed that the polysaccharide is an unbranched high molecular weight linear polymer of a partially O-acetylated pentasaccharide repeating unit having the structure indicated below.[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30)

1984 ◽  
Vol 62 (2-3) ◽  
pp. 151-161 ◽  
Author(s):  
Martine Caroff ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30) is composed of D-galactose (three parts), D-glucose (one part), 2-acetamido-2-deoxy-D-glucose (one part), phosphate (one part), and glycerol (one part). Hydrolysis, periodate oxidation, methylation, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight linear polymer of a pentasaccharide repeating unit having the structure:[Formula: see text]

The structures of the two lipopolysaccharide O-chains produced by Salmonella boecker

1988 ◽  
Vol 66 (10) ◽  
pp. 1066-1077 ◽  
Author(s):  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Nitrous Acid ◽  
Optical Rotation ◽  
Partial Hydrolysis ◽  
Magnetic Resonance Studies ◽  
High Molecular Weight Polymers ◽  
Nuclear Magnetic

Salmonella boecker, which belongs to group 0:6, 14(H) and shows the antigenic factors 6, 14, [1], and [25], defined by the Kauffmann–White system, produces two lipopolysaccharides differing from each other in the structures of their 0-polysaccharide moieties. By glycose composition, partial hydrolysis, nitrous acid deamination, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies, the O-polysaccharides were demonstrated to be high-molecular-weight polymers (I and II) composed of either structurally related repeating tetrasaccharide or repeating pentasaccharide units having the structuresand[Formula: see text][Formula: see text]

Structure of the exocellular D-glucan produced by Neisseria polysaccharea

1986 ◽  
Vol 32 (12) ◽  
pp. 909-911 ◽  
Author(s):  
Jean-Yves Riou ◽  
Martine Guibourdenche ◽  
Malcolm B. Perry ◽  
Leann L. MacLan ◽  
Douglas W. Griffith
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Optical Rotation ◽  
Prototype Strain ◽  
New Taxon ◽  
Branch Points ◽  
Enzymic Hydrolysis ◽  
Specific Optical Rotation ◽  
Magnetic Resonance Studies ◽  
Exocellular Polysaccharide

Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1–5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [α]D +222° (water), and was shown from composition, specific optical rotation, methylation, enzymic hydrolysis, and 13C nuclear magnetic resonance studies to have an amylopectinlike structure containing mainly 1,4-iinked α-D-glucopyranosyl residues, but also containing ca. 6% 4,6-di-O-substituted α-D-glucopyranosyl branch points.

scholarly journals Characterization and Biological Role of the O-Polysaccharide Gene Cluster of Yersinia enterocolitica Serotype O:9

2007 ◽  
Vol 189 (20) ◽  
pp. 7244-7253 ◽  
Author(s):  
Mikael Skurnik ◽  
Marta Biedzka-Sarek ◽  
Peter S. Lübeck ◽  
Tea Blom ◽  
José Antonio Bengoechea ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Yersinia Enterocolitica ◽  
Lipid A ◽  
Inner Core ◽  
Attachment Site ◽  
Polymyxin B ◽  
Outer Core ◽  
Core Oligosaccharide ◽  
Serotype O

ABSTRACTYersinia enterocoliticaserotype O:9 is a gram-negative enteropathogen that infects animals and humans. The role of lipopolysaccharide (LPS) inY. enterocoliticaO:9 pathogenesis, however, remains unclear. The O:9 LPS consists of lipid A to which is linked the inner core oligosaccharide, serving as an attachment site for both the outer core (OC) hexasaccharide and the O-polysaccharide (OPS; a homopolymer ofN-formylperosamine). In this work, we cloned the OPS gene cluster of O:9 and identified 12 genes organized into four operons upstream of thegndgene. Ten genes were predicted to encode glycosyltransferases, the ATP-binding cassette polysaccharide translocators, or enzymes required for the biosynthesis of GDP-N-formylperosamine. The two remaining genes within the OPS gene cluster,galFandgalU, were not ascribed a clear function in OPS biosynthesis; however, the latter gene appeared to be essential for O:9. The biological functions of O:9 OPS and OC were studied using isogenic mutants lacking one or both of these LPS parts. We showed that OPS and OC confer resistance to human complement and polymyxin B; the OPS effect on polymyxin B resistance could be observed only in the absence of OC.

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F

1984 ◽  
Vol 62 (8) ◽  
pp. 666-677 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F (American type 70) is composed of D-galactose (5 parts), D-glucose (1 part), and O-acetyl (ca. 0.4 parts). Periodate oxidation, partial hydrolysis, and 1H and 13C nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight polymer of a repeating hexasaccharide unit having the structure:[Formula: see text]

Structural investigation of the lipopolysaccharide core isolated from a virulent strain of Vibrio ordalii

1985 ◽  
Vol 63 (12) ◽  
pp. 1199-1205 ◽  
Author(s):  
Joseph H. Banoub ◽  
Howard J. Hodder
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nitrous Acid ◽  
Structural Investigation ◽  
Chromium Trioxide ◽  
Virulent Strain ◽  
Partial Hydrolysis ◽  
Methylation Analysis ◽  
Core Oligosaccharide ◽  
The Core ◽  
Vibrio Ordalii

The structure of the core oligosaccharide of Vibrio ordalii has been investigated. The studies involved the use of nuclear magnetic resonance, methylation analysis, partial hydrolysis with hydrochloric acid, nitrous acid deamination, partial hydrolysis with sulfuric acid, Smith degradation, and oxidation with chromium trioxide. As a result of these studies the following structure is proposed.[Formula: see text]

Structure of the polysaccharide chain of Pasteurella haemolytica (serotype 4) lipopolysaccharide

1984 ◽  
Vol 62 (2-3) ◽  
pp. 108-114 ◽  
Author(s):  
Malcolm B. Perry ◽  
Lorne A. Babiuk
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Cell Wall ◽  
Magnetic Resonance ◽  
Periodate Oxidation ◽  
Pasteurella Haemolytica ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Nuclear Magnetic Resonance Analysis ◽  
Resonance Analysis ◽  
Antigenic Polysaccharide

The antigenic polysaccharide side chain of the cell wall lipopolysaccharide of Pasteurella haemolytica (serotype 4) was investigated by methylation, periodate oxidation, partial hydrolysis, and 13C and 1H nuclear magnetic resonance analysis methods and was found to be a simple unbranched linear polymer composed of a disaccharide repeating unit having the structure —3)-α-D-Galp-(1—3)-β-D-Galf-(1—.

Structure of the acidic capsular polysaccharide of Cryptococcus laurentii (NRRL Y-1401)

1982 ◽  
Vol 60 (2) ◽  
pp. 124-130 ◽  
Author(s):  
Malcolm B. Perry ◽  
Ann C. Webb
Keyword(s):  
Molecular Weight ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Regular Structure ◽  
Cryptococcus Laurentii ◽  
Partial Acid Hydrolysis ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The capsular polysaccharide produced by Cryptococcus laurentii (NRRL Y-1401) is composed of D-mannose (3 mol), D-glucuronic acid (1 mol), D-xylose (1 mol), and O-acetyl (~1 mol). Methylation, periodate oxidation, partial acid hydrolysis, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight branched polymer of regular structure having a repeating pentasaccharide unit with the structure:[Formula: see text]

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