Structure of the exocellular D-glucan produced by Neisseria polysaccharea

1986 ◽  
Vol 32 (12) ◽  
pp. 909-911 ◽  
Author(s):  
Jean-Yves Riou ◽  
Martine Guibourdenche ◽  
Malcolm B. Perry ◽  
Leann L. MacLan ◽  
Douglas W. Griffith
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Optical Rotation ◽  
Prototype Strain ◽  
New Taxon ◽  
Branch Points ◽  
Enzymic Hydrolysis ◽  
Specific Optical Rotation ◽  
Magnetic Resonance Studies ◽  
Exocellular Polysaccharide

Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1–5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [α]D +222° (water), and was shown from composition, specific optical rotation, methylation, enzymic hydrolysis, and 13C nuclear magnetic resonance studies to have an amylopectinlike structure containing mainly 1,4-iinked α-D-glucopyranosyl residues, but also containing ca. 6% 4,6-di-O-substituted α-D-glucopyranosyl branch points.

Structure of the lipopolysaccharide O-chain of Yersinia enterocolitica serotype O:5,27

1987 ◽  
Vol 65 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Malcolm B. Perry ◽  
Leann L. MacLean
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Yersinia Enterocolitica ◽  
Lipid A ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Specific Optical Rotation ◽  
Core Oligosaccharide ◽  
Magnetic Resonance Studies ◽  
Serotype O

The cellular lipopolysaccharide produced by Yersinia enterocolitica serotype O:5,27 was of the S-type and composed of an antigenic O-chain polysaccharide linked through a core oligosaccharide region, which in turn was linked through 3-deoxy-D-manno-octulonosyl units to a lipid A moiety. The O-chain polysaccharide was composed of equal molar amounts of L-rhamnose and D-xylulose. By partial hydrolysis, periodate oxidation, methylation, specific optical rotation, and 13C and 1H nuclear magnetic resonance studies, the structure of the O-chain was established as being a linear backbone of alternating 1,3-linked α-L-rhamnopyranosyl and β-L-rhamnopyranosyl units, to which 2,2-linked β-D-threo-pent-2-ulofuranoside (D-xylulofuranoside) units were present on every L-rhamnopyranosyl residue, as shown below.[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30)

1984 ◽  
Vol 62 (2-3) ◽  
pp. 151-161 ◽  
Author(s):  
Martine Caroff ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30) is composed of D-galactose (three parts), D-glucose (one part), 2-acetamido-2-deoxy-D-glucose (one part), phosphate (one part), and glycerol (one part). Hydrolysis, periodate oxidation, methylation, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight linear polymer of a pentasaccharide repeating unit having the structure:[Formula: see text]

The structures of the two lipopolysaccharide O-chains produced by Salmonella boecker

1988 ◽  
Vol 66 (10) ◽  
pp. 1066-1077 ◽  
Author(s):  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Nitrous Acid ◽  
Optical Rotation ◽  
Partial Hydrolysis ◽  
Magnetic Resonance Studies ◽  
High Molecular Weight Polymers ◽  
Nuclear Magnetic

Salmonella boecker, which belongs to group 0:6, 14(H) and shows the antigenic factors 6, 14, [1], and [25], defined by the Kauffmann–White system, produces two lipopolysaccharides differing from each other in the structures of their 0-polysaccharide moieties. By glycose composition, partial hydrolysis, nitrous acid deamination, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies, the O-polysaccharides were demonstrated to be high-molecular-weight polymers (I and II) composed of either structurally related repeating tetrasaccharide or repeating pentasaccharide units having the structuresand[Formula: see text][Formula: see text]

Structure of the D-glucans produced by Neisseria perflava

1978 ◽  
Vol 24 (11) ◽  
pp. 1419-1422 ◽  
Author(s):  
C. R. MacKenzie ◽  
M. B. Perry ◽  
I. J. McDonald ◽  
K. G. Johnson
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Optical Rotation ◽  
Side Chains ◽  
Cell Free Extract ◽  
Branch Points ◽  
Methylation Analysis ◽  
A Cell ◽  
Neisseria Perflava ◽  
Nuclear Magnetic

A chemical and enzymic study of the cellular glucan of Neisseria perflava and of the glucan produced from sucrose by a cell-free extract of N. perflava showed from optical rotation, 13C nuclear magnetic resonance, methylation analysis, and α- and β-amylase hydrolysis studies that the glucans had glycogen-like structures. These structures were composed of chains of 1-4-α-D-glucopyranosyl units with branch points of 4-O- and 6-O- disubstituted D-glucopyranose units. While the backbone structures of the two glucans were similar, the release of maltose by the action of β-amylase indicated that the 1–4 linked nonreducing side chains of the cell-free enzymically synthesized glucan were longer (~seven units) than those present in the cellular glucan (~two to three units), a result in agreement with methylation analyses.

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