Thymus metallothionein: regulation of zinc-thionein in the aging mouse

1985 ◽  
Vol 63 (2) ◽  
pp. 91-95 ◽  
Author(s):  
R. W. Olafson
Keyword(s):  
New Zealand ◽  
Early Onset ◽  
Molecular Basis ◽  
Mouse Thymus ◽  
Age Related ◽  
Thymus Tissue ◽  
Aging Mouse ◽  
Zinc Concentrations ◽  
Zinc Levels ◽  
Immune Dysfunctions

The presence of a zinc-inducible metallothionein in mouse thymus tissue has been shown, together with an age-related modulation of zinc, metallothionein, and thymus mass. Metallothionein (MT) and zinc concentrations were found to be maximum prior to completion of thymic growth and to be at a minimum when development of the thymus had ceased. Subsequent transient outgrowths of the thymus during involution showed similar associated fluctuations of MT and zinc levels. A marked difference in the latter parameters was found on comparison with New Zealand Black mice known for development of early onset autoimmunities. These findings provide a new avenue for both investigation of the regulation of zinc in thymus tissue, as well as the possible elucidation of the molecular basis for certain age-related immune dysfunctions.

scholarly journals Temperature-dependent structural and functional properties of a mutant (F71L) αA-crystallin: Molecular basis for early onset of age-related cataract

FEBS Letters ◽  
2011 ◽  
Vol 585 (24) ◽  
pp. 3884-3889 ◽  
Author(s):  
Vakdevi Validandi ◽  
V. Sudhakar Reddy ◽  
P.N.B.S. Srinivas ◽  
Niklaus H. Mueller ◽  
S.G. Bhagyalaxmi ◽  
...  
Keyword(s):  
Functional Properties ◽  
Early Onset ◽  
Molecular Basis ◽  
Temperature Dependent ◽  
Age Related ◽  
Structural And Functional Properties

Evaluation of Metal Contents in Correlation with Phytosanitary Treatments at Vineyard

2017 ◽  
Vol 68 (6) ◽  
pp. 1289-1293
Author(s):  
Oana Mihai ◽  
Octav Pantea ◽  
Daniela Roxana Popovici ◽  
Catalina Gabriela Gheorghe
Keyword(s):  
Soil Samples ◽  
Flame Atomic Absorption ◽  
Mobile Forms ◽  
Before And After ◽  
Phytosanitary Treatment ◽  
Zinc Concentrations ◽  
Zinc Levels ◽  
Ph Range ◽  
In The Beginning ◽  
Short Time

The present work aims with the evaluation of copper, manganese and zinc concentrations (mobile forms) from vineyard soil before and after phytosanitary treatment with Curzate Manox and Dithane M-45 compounds, during and after remanence period. Different vineyard soils types were collected at 0-20 cm and 20-40 cm depths. Flame Atomic Absorption Spectroscopy (FAAS) method was used for measurements of the micronutrients. The soil samples were analyzed after 5 and 21 days after treatment application. Since copper is mainly accumulates in the upper layer following fungicidal sprays application, high levels of copper concentrations are obtained. The soil samples exhibits different behavior in terms of manganese and zinc contents. Manganese and zinc levels are classified as medium in the beginning of the experiment (Mn-M0 and Zn-M0), whereas these levels increased in the soil samples (at moments M1- 5 days and M2- 21 days after treatment). This behavior can be due to the Mancozeb decomposition, knowing that Mancozeb decomposes in the pH range 5-9 and it remains short time into the soil.

Association of Different Zinc Concentrations Combined with a Fixed Caffeine Dose on Plasma and Tissue Caffeine and Zinc Levels in the Rat

2002 ◽  
Vol 87 (1-3) ◽  
pp. 183-190 ◽  
Author(s):  
Malektaj Yazdani ◽  
Sheila Gottschalk ◽  
Kazuya Ide ◽  
Tetsuo Nakamoto
Keyword(s):  
Zinc Concentrations ◽  
Zinc Levels

Methane emissions from young and mature dairy cattle

2016 ◽  
Vol 56 (11) ◽  
pp. 1897 ◽  
Author(s):  
C. A. Ramírez-Restrepo ◽  
H. Clark ◽  
S. Muetzel
Keyword(s):  
New Zealand ◽  
Emission Factor ◽  
Dry Matter ◽  
Sulfur Hexafluoride ◽  
Dry Matter Intake ◽  
Tracer Technique ◽  
Ch4 Emissions ◽  
Dairy Heifers ◽  
Age Related ◽  
The Mean

Daily methane (CH4) emissions (g) and CH4 yield (g/kg dry matter intake) were measured from 10 dairy heifers (<1 year old) and nine rumen-fistulated cows (>6 years old) fed ryegrass (Lolium perenne) chaffage indoors. The CH4 emissions were estimated using the sulfur hexafluoride tracer technique in four ~5-day periods beginning in June 2008 and repeated 4, 6 and 7 months later. Respiratory chambers were used in four ~13-day periods beginning in November 2008 and repeated 2, 5 and 6 months later. Third and fourth sulfur hexafluoride tracer periods overlapped with the first and second chamber measurement periods, respectively. Averaged over the four measurement periods the CH4 yields determined using both techniques were similar for heifers and cows. The mean CH4 yield estimated by the sulfur hexafluoride tracer technique was 25.3 ± 0.52 for heifers and 24.1 ± 0.55 for mature cows, whereas the mean CH4 yield measured in respiratory chambers was 23.7 ± 0.66 for heifers and 23.6 ± 0.66 for mature cows. Averaged over the eight measurements irrespective of technique, CH4 yields for heifers (24.5 ± 0.42) and cows (23.8 ± 0.43) were similar. There was also no difference between CH4 methods for assessing CH4 yield during the overlapping measurement periods. It was concluded that no consistent differences in CH4 yield existed between heifers and mature cows. Therefore, we do not recommend adoption of an age-related emission factor for cattle in the national inventory calculations for New Zealand.

scholarly journals Loss of sestrin 2 potentiates the early onset of age-related sensory cell degeneration in the cochlea

Neuroscience ◽  
2017 ◽  
Vol 361 ◽  
pp. 179-191 ◽  
Author(s):  
Celia Zhang ◽  
Wei Sun ◽  
Ji Li ◽  
Binbin Xiong ◽  
Mitchell D. Frye ◽  
...  
Keyword(s):  
Early Onset ◽  
Sensory Cell ◽  
Cell Degeneration ◽  
Age Related

scholarly journals Early-Onset Parkinson Disease Screening in Patients From Nigeria

2021 ◽  
Vol 11 ◽  
Author(s):  
Lukasz M. Milanowski ◽  
Olajumoke Oshinaike ◽  
Benjamin J. Broadway ◽  
Jennifer A. Lindemann ◽  
Alexandra I. Soto-Beasley ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Parkinson Disease ◽  
Neurodegenerative Diseases ◽  
Early Onset ◽  
Local Population ◽  
Age At Onset ◽  
Compound Heterozygous ◽  
Loss Of Function ◽  
African Countries ◽  
Age Related

Introduction: Nigeria is one of the most populated countries in the world; however, there is a scarcity of studies in patients with age-related neurodegenerative diseases, such as Parkinson disease (PD). The aim of this study was to screen patients with PD including a small cohort of early-onset PD (EOPD) cases from Nigeria for PRKN, PINK1, DJ1, SNCA multiplication, and LRRK2 p.G2019S.Methods: We assembled a cohort of 109 Nigerian patients with PD from the four main Nigerian tribes: Yoruba, Igbo, Edo, and Hausa. Fifteen cases [14 from the Yoruba tribe (93.3%)] had EOPD (defined as age-at-onset &lt;50 years). All patients with EOPD were sequenced for the coding regions of PRKN, PINK1, and DJ1. Exon dosage analysis was performed with a multiplex ligation-dependent probe amplification assay, which also included a SNCA probe and LRRK2 p.G2019S. We screened for LRRK2 p.G2019S in the entire PD cohort using a genotyping assay. The PINK1 p.R501Q functional analysis was conducted.Results: In 15 patients with EOPD, 22 variants were observed [PRKN, 9 (40.9%); PINK1, 10 (45.5%); and DJ1, 3 (13.6%)]. Three (13.6%) rare, nonsynonymous variants were identified, but no homozygous or compound heterozygous carriers were found. No exonic rearrangements were present in the three genes, and no carriers of SNCA genomic multiplications or LRRK2 p.G2019S were identified. The PINK1 p.R501Q functional analysis revealed pathogenic loss of function.Conclusion: More studies on age-related neurodegenerative diseases are needed in sub-Saharan African countries, including Nigeria. Population-specific variation may provide insight into the genes involved in PD in the local population but may also contribute to larger studiesperformed in White and Asian populations.

Measurement of Hypozincaemia in Mice: A Sensitive Test for Detection of Pyrogens

1981 ◽  
Vol 61 (4) ◽  
pp. 445-449 ◽  
Author(s):  
Susan Boobis ◽  
Rose E. Hartley
Keyword(s):  
Escherichia Coli ◽  
Zinc Concentration ◽  
Shigella Dysenteriae ◽  
Metabolic Inhibitor ◽  
Repeated Treatment ◽  
Plasma Zinc ◽  
Sensitive Test ◽  
Zinc Concentrations ◽  
Zinc Levels ◽  
Dose Dependent

1. The effect of bacterial pyrogens on plasma zinc concentration in mice was studied as a method of bioassay for these substances. 2. A dose-related depression of plasma zinc concentrations was observed 4 h after intravenous injection of doses of 0.05-500 ng of purified endotoxins of Salmonella abortus equi, Shigella dysenteriae and Escherichia coli. Responses were dose-dependent and reproducible for each endotoxin in five strains of mice. 3. Tolerance to endotoxin could be induced in mice by injection of doses of 500 ng but was not seen with doses of <5 ng, even after repeated treatment. 4. Dinitrophenol, a metabolic inhibitor, and amphetamine, a metabolic enhancer, did not affect plasma zinc levels, indicating that changes in basal metabolic rate do not affect the outcome of this assay. The effects of pyrogenic materials other than endotoxin on plasma zinc levels were also tested and the results suggest that measurement of hypozincaemia in mice provides the basis for a simple, practical and inexpensive test for endotoxins and other pyrogens.

Age-related insult of cochlear ribbon synapses: An early-onset contributor to D-galactose-induced aging in mice

2020 ◽  
Vol 133 ◽  
pp. 104649 ◽  
Author(s):  
Zheng-De Du ◽  
Shu-Guang Han ◽  
Teng-Fei Qu ◽  
Bin Guo ◽  
Shu-Kui Yu ◽  
...  
Keyword(s):  
Early Onset ◽  
Ribbon Synapses ◽  
Age Related

scholarly journals Ultrarare heterozygous pathogenic variants of genes causing dominant forms of early-onset deafness underlie severe presbycusis

2020 ◽  
Vol 117 (49) ◽  
pp. 31278-31289
Author(s):  
Sophie Boucher ◽  
Fabienne Wong Jun Tai ◽  
Sedigheh Delmaghani ◽  
Andrea Lelli ◽  
Amrit Singh-Estivalet ◽  
...  
Keyword(s):  
Risk Factors ◽  
Hearing Loss ◽  
Early Onset ◽  
Cell Biology ◽  
Public Health Issue ◽  
Phenotypic Variance ◽  
Pathogenic Variants ◽  
Age Related ◽  
Small Effect Size ◽  
Age Related Hearing Loss

Presbycusis, or age-related hearing loss (ARHL), is a major public health issue. About half the phenotypic variance has been attributed to genetic factors. Here, we assessed the contribution to presbycusis of ultrarare pathogenic variants, considered indicative of Mendelian forms. We focused on severe presbycusis without environmental or comorbidity risk factors and studied multiplex family age-related hearing loss (mARHL) and simplex/sporadic age-related hearing loss (sARHL) cases and controls with normal hearing by whole-exome sequencing. Ultrarare variants (allele frequency [AF] < 0.0001) of 35 genes responsible for autosomal dominant early-onset forms of deafness, predicted to be pathogenic, were detected in 25.7% of mARHL and 22.7% of sARHL cases vs. 7.5% of controls (P= 0.001); half were previously unknown (AF < 0.000002).MYO6,MYO7A,PTPRQ, andTECTAvariants were present in 8.9% of ARHL cases but less than 1% of controls. Evidence for a causal role of variants in presbycusis was provided by pathogenicity prediction programs, documented haploinsufficiency, three-dimensional structure/function analyses, cell biology experiments, and reported early effects. We also establishedTmc1N321I/+mice, carrying theTMC1:p.(Asn327Ile) variant detected in an mARHL case, as a mouse model for a monogenic form of presbycusis. Deafness gene variants can thus result in a continuum of auditory phenotypes. Our findings demonstrate that the genetics of presbycusis is shaped by not only well-studied polygenic risk factors of small effect size revealed by common variants but also, ultrarare variants likely resulting in monogenic forms, thereby paving the way for treatment with emerging inner ear gene therapy.

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