Erratum: Expansion of phospholipid pool size of rat intestinal villus cells during fat absorption

1983 ◽  
Vol 61 (4) ◽  
pp. 229-229
Author(s):  
N. A. Shaikh ◽  
A. Kuksis
Keyword(s):  
Pool Size ◽  
Fat Absorption ◽  
Intestinal Villus ◽  
Phospholipid Pool

Expansion of phospholipid pool size of rat intestinal villus cells during fat absorption

1982 ◽  
Vol 60 (4) ◽  
pp. 444-451 ◽  
Author(s):  
N. A. Shaikh ◽  
A. Kuksis
Keyword(s):  
Phospholipid Composition ◽  
Pool Size ◽  
Cell Protein ◽  
Phospholipid Content ◽  
Fat Absorption ◽  
Mucosal Cells ◽  
Microsomal Membranes ◽  
Single Meal ◽  
Intestinal Mucosal Cells ◽  
Phospholipid Pool

The effect of fat absorption upon the phospholipid pool size of the intestinal mucosal cells was determined in rats receiving fatty emulsions as a bolus by stomach tube or as multiple meals in the form of fat-laden laboratory chow. The phospholipid content of the mucosal scrapings and of the isolated villus cells was determined 3 to 34 h after the meals and was compared with the phospholipid content of cells from similar animals receiving water alone or 10% sucrose in water. It was shown that continuously fed animals averaged 5–10% and single meal fed animals up to 40% higher phospholipid content in their mucosal cells than the corresponding controls, when compared per milligram cell protein. The expansion of the phospholipid pool involved all phospholipid classes and correlated well with the phospholipid composition of prechylomicrons and of microsomal membranes, which undergo a significant proliferation during fat absorption. The apparent lower expansion of the phospholipid pool in the continuously fed animals correlated with the lower triacylglycerol content of the lumen and of the cells at these times.

Glutathione Replacement Preserves the Functional Surfactant Phospholipid Pool Size and Decreases Sepsis-Mediated Lung Dysfunction in Ethanol-Fed Rats

2002 ◽  
Vol 26 (8) ◽  
pp. 1245-1251 ◽  
Author(s):  
Alvaro Velasquez ◽  
Rabih I. Bechara ◽  
James F. Lewis ◽  
Jaret Malloy ◽  
Lynda McCaig ◽  
...  
Keyword(s):  
Pool Size ◽  
Lung Dysfunction ◽  
Phospholipid Pool

scholarly journals Lung surfactant in a cystic fibrosis animal model: increased alveolar phospholipid pool size without altered composition and surface tension function in cftrm1HGU/m1HGU mice

Thorax ◽  
1997 ◽  
Vol 52 (8) ◽  
pp. 723-730 ◽  
Author(s):  
W. Bernhard ◽  
J. Y. Wang ◽  
T. Tschernig ◽  
B. Tummler ◽  
H. J. Hedrich ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Surface Tension ◽  
Animal Model ◽  
Lung Surfactant ◽  
Pool Size ◽  
Phospholipid Pool

Further evidence for enhanced phospholipid synthesis by rat jejunal villus cells during fat absorption

1983 ◽  
Vol 61 (6) ◽  
pp. 370-377 ◽  
Author(s):  
N. A. Shaikh ◽  
A. Kuksis
Keyword(s):  
De Novo ◽  
Corn Oil ◽  
Specific Activity ◽  
Chow Diet ◽  
Fat Absorption ◽  
Membrane Phospholipids ◽  
Relative Specific Activity ◽  
Phospholipid Classes ◽  
Mucosal Cells ◽  
Phospholipid Pool

The effect of fat absorption on the phospholipid turnover of rat intestinal mucosa was determined in animals receiving single fatty meals by stomach tube or multiple meals in the form of corn-oil-soaked laboratory chow diet. The specific activity and relative specific activity of the total phospholipids and of individual phospholipid classes were measured in the isolated jejunal villus cells of fasting and fat-fed animals following an injection of radioactive inorganic phosphate 0.5–31 h prior to sacrifice, which was scheduled to coincide with the peak of fat absorption (2.5–3 h after the last meal). It was shown that the relative specific activity of the fat-absorbing cells increased by about 33% when the samples were taken 0.5 h after intravenous injection of radioactive phosphate. Samples taken 11 and 31 h after the introduction of the radioactive phosphate showed about 16% decrease in the relative specific activity of the phospholipids of the fat-absorbing cells when compared with the fasting controls. These changes in the relative specific activity of the total phospholipids included all phospholipid classes and corresponded to the recently described expansion of the cellular phospholipid pool owing partly to increased de novo synthesis of the membrane phospholipids. The present results are consistent with the known biochemical and physiological changes taking place in the mucosal cells during fat absorption and transport and find support in various less direct biochemical and morphometric measurements.

A Study of Carbohydrate and Fat Absorption from the Normal and Diseased Intestine in Man

1952 ◽  
Vol 20 (2) ◽  
pp. 287-293 ◽  
Author(s):  
F.S. Brien ◽  
D.A. Turner ◽  
E.M. Watson ◽  
J.H. Geddes
Keyword(s):  
Fat Absorption

Research Recommendations for Applying Vitamin A-Labelled Isotope Dilution Techniques to Improve Human Vitamin A Nutrition

2014 ◽  
Vol 84 (Supplement 1) ◽  
pp. 52-59 ◽  
Author(s):  
Sherry A. Tanumihardjo ◽  
Anura V. Kurpad ◽  
Janet R. Hunt
Keyword(s):  
Public Health ◽  
Vitamin A ◽  
Vitamin A Deficiency ◽  
Human Subjects ◽  
The Body ◽  
Pool Size ◽  
Serum Retinol ◽  
Vitamin A Status ◽  
Status Assessment ◽  
Total Body

The current use of serum retinol concentrations as a measurement of subclinical vitamin A deficiency is unsatisfactory for many reasons. The best technique available for vitamin A status assessment in humans is the measurement of total body pool size. Pool size is measured by the administration of retinol labelled with stable isotopes of carbon or hydrogen that are safe for human subjects, with subsequent measurement of the dilution of the labelled retinol within the body pool. However, the isotope techniques are time-consuming, technically challenging, and relatively expensive. There is also a need to assess different types of tracers and doses, and to establish clear guidelines for the use and interpretation of this method in different populations. Field-friendly improvements are desirable to encourage the application of this technique in developing countries where the need is greatest for monitoring the risk of vitamin A deficiency, the effectiveness of public health interventions, and the potential of hypervitaminosis due to combined supplement and fortification programs. These techniques should be applied to validate other less technical methods of assessing vitamin A deficiency. Another area of public health relevance for this technique is to understand the bioconversion of β-carotene to vitamin A, and its relation to existing vitamin A status, for future dietary diversification programs.

scholarly journals ON FAT ABSORPTION

1912 ◽  
Vol 11 (4) ◽  
pp. 429-434
Author(s):  
W.R. Bloor
Keyword(s):  
Fat Absorption
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