Quantitative analysis of purine and pyrimidine metabolism in Chinese hamster ovary cells

1981 ◽  
Vol 59 (10) ◽  
pp. 838-847 ◽  
Author(s):  
Darel Hunting ◽  
Joyce Hordern ◽  
J. Frank Henderson
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Study ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Pyrimidine Metabolism ◽  
Ovary Cells ◽  
Direct Measurements ◽  
Deoxyribonucleoside Triphosphate ◽  
Pool Sizes

A detailed quantitative study of purine and pyrimidine metabolism in logarithmically growing Chinese hamster ovary cells has been performed. Direct measurements of the ribonucleoside and deoxyribonucleoside triphosphate pool sizes have been made, and the pathways and rates of metabolism of adenine, hypoxanthine, guanine, guanosirae, deoxyadenosine, and deoxyguanosine and of uridine, cytidine, deoxyeytidine, deoxyuridine, and thymidine have been determined.

Comparison of purine and pyrimidine metabolism in G1 and S phases of HeLa and Chinese hamster ovary cells

1982 ◽  
Vol 60 (4) ◽  
pp. 422-433 ◽  
Author(s):  
Joyce Hordern ◽  
J. Frank Henderson
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
S Phase ◽  
Pyrimidine Metabolism ◽  
Ovary Cells ◽  
Size Dependent ◽  
Quantitative Studies ◽  
Deoxyribonucleoside Triphosphate ◽  
Rna And Dna

Detailed quantitative studies of purine and pyrimidine metabolism in G1 and S phases of synchronized HeLa and Chinese hamster ovary cells have been carried out. Concentrations of ribonucleoside triphosphates increased approximately in proportion to the increase in cell size as cells moved from G1 to S phase. Deoxyribonucleoside triphosphate concentrations were low in G1 phase and increased 2.5- to 10-fold in S phase. Pathways and rates of metabolism of radioactive adenine, guanosine, deoxyadenosine, deoxyguanosine, uridine, cytidine, deoxyuridine, deoxycytidine, and thymidine were determined by measuring the incorporation of each precursor into individual acid-soluble nucleotides and RNA and DNA bases. Cell-cycle or size-dependent differences were detected in many of the parameters studied.

Effects of altered ribonucleotide concentrations on ribonucleotide reduction in intact Chinese hamster ovary cells

1981 ◽  
Vol 59 (10) ◽  
pp. 821-829 ◽  
Author(s):  
Darel Hunting ◽  
Joyce Hordern ◽  
J. Frank Henderson
Keyword(s):  
Chinese Hamster Ovary ◽  
Narrow Range ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Naturally Occurring ◽  
Wide Range ◽  
Ribonucleoside Triphosphate ◽  
Deoxyribonucleoside Triphosphate ◽  
The Relationship

The effect of changes in ribonucleoside triphosphate concentrations on those of deoxyribonucleotides has been determined in Chinese hamster ovary K-1 cells. Ribonucleoside triphosphate concentrations were manipulated by the use of drugs or naturally occurring purines or pyrimidines. Changes in the concentration of each ribonucleoside triphosphate led to changes in the concentration of each corresponding deoxyribonucleoside triphosphate. The relationships between the concentrations of CTP and dCTP and between those of GTP and dGTP were linear over a wide range. The relationship between the concentrations of ATP and dATP was linear over the narrow range studied, while the relationship between UTP and dTTP concentrations was curvilinear. Overall, these results demonstrate that ribonucleotides play an important role in controlling deoxyribonucleotide concentrations but the exact mechanism of this control remains to be determined.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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