Resolution of ribonucleic acids by Sepharose 4B column chromatography

Biochemistry ◽  
1977 ◽  
Vol 16 (7) ◽  
pp. 1378-1382 ◽  
Author(s):  
Margarita Zeichner ◽  
Robert Stern
Keyword(s):  
Column Chromatography ◽  
Ribonucleic Acids ◽  
Sepharose 4B

Fractionation of Escherichia coli isoaccepting tRNA species by Sepharose 4B Column chromatography

1979 ◽  
Vol 93 ◽  
pp. 248-250 ◽  
Author(s):  
Vittorio Colantuoni ◽  
Ludovico Guarini ◽  
Riccardo Cortese
Keyword(s):  
Escherichia Coli ◽  
Column Chromatography ◽  
Trna Species ◽  
Sepharose 4B

scholarly journals Purification and properties of acid phosphatase from Avena elatior L. seeds

2015 ◽  
Vol 46 (3) ◽  
pp. 481-488 ◽  
Author(s):  
E. Wieczorek ◽  
I. Lorenc-Kubis ◽  
B. Morawiecka
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Column Chromatography ◽  
Gel Filtration ◽  
Ph Optimum ◽  
Purification And Properties ◽  
Sepharose 4B

Acid phosphatase F1 from <i>Avena elatior</i> seeds was isolated and partially purified by means of alcohol precepitation, DEAE-, CM-column chromatography, Sephadex G-150, Sephadex G-200 and Sepharose 4B - gel filtration. The enzyme was stable at 50°C, pH 5.1. The pH optimum for phosphatase activity was 4.2. Fluoride, Zn<sup>2+</sup>, molybdate were effective inhibitors. EDTA and l, 10-phenanthroline activated the enzyme.

Immunoenzymometric assay for insulin involving column chromatography and insulin immobilized on Sepharose.

1985 ◽  
Vol 31 (4) ◽  
pp. 628-630 ◽  
Author(s):  
R Yamamoto ◽  
S Kimura ◽  
S Hattori ◽  
A Matsuura ◽  
T Hayakawa
Keyword(s):  
Enzyme Immunoassay ◽  
Sodium Carbonate ◽  
Column Chromatography ◽  
Sodium Carbonate Solution ◽  
Solid Phase ◽  
Enzyme Reaction ◽  
Carbonate Solution ◽  
Chromatographic Column ◽  
Serum Samples ◽  
Sepharose 4B

Abstract This practical assay for measuring insulin involves use of a 4 X 8 mm chromatographic column. Serum samples are incubated at 30 degrees C for 1 h with beta-D-galactosidase-labeled antibody to insulin, then passed through the 0.1-mL column containing insulin immobilized on Sepharose 4B. After the column is washed to remove the bound label, the buffer in the column is replaced with a solution of o-nitrophenyl-beta-D-galactoside. The column is then incubated at 30 degrees C for 1 h, the enzyme reaction is stopped by washing the column with sodium carbonate solution, and the absorbance of the eluate is measured at 420 nm. Results obtained by this method were compared with those by a radioimmunoassay and a solid-phase enzyme immunoassay.

The purification and properties of valine tRNAs of Drosophila melanogaster

1978 ◽  
Vol 56 (6) ◽  
pp. 618-623 ◽  
Author(s):  
Robert Dunn ◽  
W. R. Addison ◽  
I. C. Gillam ◽  
G. M. Tener
Keyword(s):  
Drosophila Melanogaster ◽  
Column Chromatography ◽  
Base Composition ◽  
The Other ◽  
Ribonucleic Acids ◽  
Purification And Properties ◽  
Major Species

The valine transfer ribonucleic acids of Drosophila melanogaster have been purified by column chromatography on BD-cellulose, Sepharose 6B, and RPC-5. Three major species were analyzed for base composition and coding properties. [Formula: see text] binds strongly to ribosomes in the presence of GUA and to a lesser extent with GUU and GUG. [Formula: see text] binds strongly in the presence of GUG and very poorly if at all with the other three triplets whereas [Formula: see text], which contains inosine, binds strongly in the presence of GUU, GUC, and GUA and weakly with GUG.

Vergleichende Analyse der nach den Extraktionsmethoden von Kirby und Georgiev aus Mäuseleber extrahierten RNS-Fraktionen / Analysis of the RNA Fractions of Mouse Liver: Comparison of the Extraction Methods of Kirby and Georgiev

1971 ◽  
Vol 26 (3) ◽  
pp. 235-243 ◽  
Author(s):  
W. Hennig ◽  
W. Kunz ◽  
B. Schnieders ◽  
K. Williams
Keyword(s):  
Comparative Analysis ◽  
Nucleic Acid ◽  
Acid Composition ◽  
Column Chromatography ◽  
Mouse Liver ◽  
Extraction Methods ◽  
Ribonucleic Acids ◽  
Ca 50 ◽  
Liver Homogenates ◽  
Labeled Rna

Ribonucleic acids labeled with 14C- or 3H-orotic acids, respectively were extracted from aliquots of mouse liver homogenates according to the cold-phenol-method of Kirby or the method of Georgiev employing different temperature steps.This comparative analysis showed that only ca. 50% of the total labeled RNA was extracted by the Kirby method. Especially the high molecular D-RNA and R-RNA were not sufficiently extracted, as was demonstrated by hydroxyapatite- and methylalbumin-Kieselgur column chromatography.Reextraction of the tissue at 65 °C first extracted by the Kirby method resulted in a recovery of most of the missing nucleic acid species.Therefore, results obtained by the Kirby and Georgiev methods will be different in regard to the nucleic acid composition. These findings could explain contradictary results about this problem in literature.

Microscopic investigations of novel intracellular bodies of a Nocardia SP

1994 ◽  
Vol 52 ◽  
pp. 356-357
Author(s):  
J. L. Stites
Keyword(s):  
Uranyl Acetate ◽  
Electron Microscopic ◽  
En Bloc ◽  
Ribonucleic Acids ◽  
Transmission Electron ◽  
Nocardia Sp ◽  
Internal Constitution ◽  
Membrane Bound ◽  
Molecular Components ◽  
Protein Rich

A Nocardia sp.was found during an initial transmission electron microscopic (TEM) examination to have unusual intracellular bodies (ICB's) which do not appear to have been described previously in the literature. Most intracellular structures within bacteria have been classified as storage granules, a product of membrane invagination (i.e. mesosomes), or vacuoles. In bacteria there are no known intracellular membrane-bound organelles, and all internal membranes are invaginations of the unit membrane. Several microscopic-level examinations of the Nocardia sp. ICB's were initiated in order to determine their overall structure, classification, and internal constitution.Different TEM staining procedures were performed to determine possible molecular components of the ICB. In all of the staining protocols the ICB's showed a lack of electron density similar to the cell wall. Because the ICB's showed no affinity to any stain, it appeared they do not have strong positive charge (phosphotungstic acid), are not protein rich (en bloc uranyl acetate), lack glycogen and are not phosphate or sulphur rich (lead citrate), nor do they contain lipids or ribonucleic acids (osmium tetroxide).

Electronmicroscopic localization of ribonucleic acids in ciliate Bursaria truncatella during cell division

1990 ◽  
Vol 48 (3) ◽  
pp. 132-133
Author(s):  
Vladimir Popenko ◽  
Natalya Cherny ◽  
Maria Yakovleva
Keyword(s):  
Cell Division ◽  
High Specificity ◽  
Longitudinal Axis ◽  
Gold Complexes ◽  
Somatic Nucleus ◽  
Ribonucleic Acids ◽  
Biological Meaning ◽  
Bivalent Cations ◽  
Lowicryl K4m ◽  
Short Time

Highly polyploid somatic nucleus (macronucleus) of ciliate Bursaria truncatella under goes severe changes in morphology during cell division. At first, macronucleus (Ma) condences, diminishes in size and turns perpendicular to longitudinal axis of the cell. After short time, Ma turns again, elongates and only afterwards the process of division itself occurs. The biological meaning of these phenomena is not clear.Localization of RNA in the cells was performed on sections of ciliates B. truncatella, embedded in “Lowicryl K4M” at various stages: (1) before cell division (Figs. 2,3); (11) at the stage of macronucleus condensation; (111) during elongation of Ma (Fig.4); (1111) in young cells (0-5min. after division). For cytochemical labelling we used RNaseAcolloidal gold complexes (RNase-Au), which are known to bind to RNA containing cell ularstructures with high specificity. The influence of different parameters on the reliability and reproducibility of labelling was studied. In addition to the factors, discussed elsewhere, we found that the balance of mono- and bivalent cations is of great significance.

Heparin Excretion in Intact and Hepatectomized Rats

1981 ◽  
Vol 45 (02) ◽  
pp. 146-149
Author(s):  
Ray Losito ◽  
Harry Gattiker ◽  
Ginette Bilodeau
Keyword(s):  
Column Chromatography ◽  
Normal Values ◽  
Anticoagulant Activity ◽  
Biliary Fistula ◽  
Kinetics Of ◽  
Intact Rats

SummaryMetabolism and kinetics of 3H-heparin were compared in intact and hepatectomized rats. Rats were divided into three groups: 1) intact rats with biliary fistulas and cystostomies 2) intact rats with only cystostomies and 3) hepatectomized rats with cystostomies. Radioactivity in blood, bile and urine besides anticoagulant activity in blood and urine were examined. In addition, column chromatography of urine was used to isolate possible metabolites. Seventy percent and 80% of the radioactive dose was found in the urine of intact rats at 24 hr and 48 hr. Close to 5% of the radioactivity was found in bile or rats with a biliary fistula after 48 hr. The APTT declined to near normal values at 1 hr whether rats had a biliary fistula or not. In contrast, only 25 % of the radioactivity could be excreted into the urine of hepatectomized rats in 24 hr; the APTT did not decline as fast and at 5 hr, it was still 100 seconds. Only one radioactive component could be isolated on chromatography from all urines of these animals and appears to be similar to the original heparin. Thus, the liver has an important role to play in regulating the anticoagulant effects and excretion of heparin.

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