Utilization of dietary nucleic acid purines for nucleotide and nucleic acid synthesis in the mouse

1976 ◽  
Vol 54 (5) ◽  
pp. 500-506 ◽  
Author(s):  
Philip W. Burridge ◽  
Robin A. Woods ◽  
J. Frank Henderson
Keyword(s):  
Skeletal Muscle ◽  
Small Intestine ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Mouse Tissues

Three preparations of radioactive yeast nucleic acids were fed to mice. One was labeled predominantly in the guanine moiety, one was labeled predominantly in the adenine moiety, and in one adenine and guanine were labeled equally. Most of the nucleic acid purines produced by digestion were excreted in the urine. However, a small amount was utilized for nucleotide and nucleic acid synthesis in the mouse tissues. Small intestine, liver and skeletal muscle contained most of the purines that were retained in the tissues. Dietary nucleic acid adenine appeared to be utilized somewhat more efficiently than was dietary nucleic acid guanine.

RELATIONSHIP BETWEEN NUCLEIC ACIDS, HISTONE AND NON-HISTONE PROTEIN SYNTHESIS IN HUMAN LYMPHOCYTES STIMULATED WITH PHYTOHEMAGGLUTININ

1970 ◽  
Vol 12 (1) ◽  
pp. 151-159 ◽  
Author(s):  
Anil B. Mukherjee
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Temporal Relationship ◽  
Human Lymphocytes ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Culture Period ◽  
Histone Protein ◽  
Pha Stimulation

The temporal relationship between the synthesis of histone and non-histone proteins and nucleic acids has been investigated by autoradiography in PHA stimulated human lymphocytes throughout the 72-hour culture period. It was found that a significant amount of 3H-arginine and 3H-lysine incorporation took place at a time when the cells were actively synthesizing DNA. Non-histone protein synthesis, as evidenced by 3H-tryptophan incorporation, was found to be dependent on PHA stimulation in human lymphocytes. Inhibition of histone and/or non-histone protein synthesis leads to the inhibition of nucleic acid synthesis.

scholarly journals THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS

1960 ◽  
Vol 112 (2) ◽  
pp. 351-359 ◽  
Author(s):  
Norman Strauss
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Hela Cells ◽  
Diphtheria Toxin ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Slight Effect ◽  
Early Effect

The effect of toxin on the incorporation of uridine and thymidine into nucleic acids was investigated. P32 orthophosphate incorporation into the nucleoside phosphate fraction was also investigated. No early effect of toxin on nucleic acid synthesis was evident and only a slight effect of toxin on nucleoside phosphate formation was found prior to 1.5 hours after toxin addition. Both these processes, however, were found to be sensitive to dinitrophenol. These results are interpreted as indicating that toxin does not act by a general uncoupling of oxidation from phosphorylation. Other possible mechanisms of toxin action are proposed.

Notizen: Contribution of Monofunctional Adducts formed by Furocoum arins with DNA to the Inhibition of Nucleic Acids Synthesis

1976 ◽  
Vol 31 (3-4) ◽  
pp. 207-208 ◽  
Author(s):  
F. Baccichetti ◽  
F. Bordin ◽  
S. Marciani ◽  
F. Dall’Acqua ◽  
G. Rodighiero
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Tumor Cells ◽  
Acid Synthesis ◽  
Ehrlich Ascites Tumor ◽  
Nucleic Acid Synthesis ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites ◽  
Monofunctional Adducts

Abstract In addition to the bifunctional adducts (cross-linkages), that furocoumarins on radiation at 365 nm form in DNA, monofunctional adducts also proved able to inhibit the nucleic acid synthesis in Ehrlich ascites tumor cells.

The relative rates of synthesis of DNA, sRNA and rRNA in the endodermal region and other parts of Xenopus laevis embryos

Development ◽  
1968 ◽  
Vol 19 (3) ◽  
pp. 363-385
Author(s):  
H. R. Woodland ◽  
J. B. Gurdon
Keyword(s):  
Nucleic Acid ◽  
Xenopus Laevis ◽  
Nucleic Acids ◽  
Cell Types ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Direct Measure ◽  
Cell Type ◽  
Different Cell Types ◽  
Xenopus Laevis Embryos

The onset and rates of synthesis of the major classes of nucleic acids have been extensively studied during the development of whole frog embryos (reviews by Brown, 1965; Gurdon, 1967a). Such information is of interest because nucleic acids are the immediate products of genes, and their rates of synthesis therefore provide a direct measure of changes in gene activity. To date nucleic acid synthesis in parts of frog embryos has been analysed mainly by methods which do not distinguish different classes of RNA (e.g. Bachvarova & Davidson, 1966; Flickinger, Miyagi, Moser & Rollins, 1967). Since embryos consist of many different cell types, it is important to know to what extent the pattern of nucleic acid synthesis observed in the whole embryo is true for its individual regions, and in particular for one differentiating cell type.

The production of interferon by animal viruses

1971 ◽  
Vol 177 (1046) ◽  
pp. 17-22 ◽  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
High Multiplicity ◽  
Multiplicity Of Infection ◽  
Viral Nucleic Acid ◽  
Interferon Inducers ◽  
Adequate Stimulus

The production of interferon by animal viruses is a response to the viral nucleic acid. At high multiplicity of infection the nucleic acid of the infecting virus is an adequate stimulus, while at lower multiplicities, viral nucleic acid synthesis is necessary for subsequent interferon formation. It is not known why viral nucleic acids are such effective interferon inducers, nor is it known how their presence within the cell leads to the formation of interferon. It is most likely that the presence of the viral nucleic acids within the cytoplasm leads to a change in the nucleus, resulting in the transcription and translation of the appropriate gene for interferon.

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