RELATIONSHIP BETWEEN NUCLEIC ACIDS, HISTONE AND NON-HISTONE PROTEIN SYNTHESIS IN HUMAN LYMPHOCYTES STIMULATED WITH PHYTOHEMAGGLUTININ

1970 ◽  
Vol 12 (1) ◽  
pp. 151-159 ◽  
Author(s):  
Anil B. Mukherjee
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Temporal Relationship ◽  
Human Lymphocytes ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Culture Period ◽  
Histone Protein ◽  
Pha Stimulation

The temporal relationship between the synthesis of histone and non-histone proteins and nucleic acids has been investigated by autoradiography in PHA stimulated human lymphocytes throughout the 72-hour culture period. It was found that a significant amount of 3H-arginine and 3H-lysine incorporation took place at a time when the cells were actively synthesizing DNA. Non-histone protein synthesis, as evidenced by 3H-tryptophan incorporation, was found to be dependent on PHA stimulation in human lymphocytes. Inhibition of histone and/or non-histone protein synthesis leads to the inhibition of nucleic acid synthesis.

Utilization of dietary nucleic acid purines for nucleotide and nucleic acid synthesis in the mouse

1976 ◽  
Vol 54 (5) ◽  
pp. 500-506 ◽  
Author(s):  
Philip W. Burridge ◽  
Robin A. Woods ◽  
J. Frank Henderson
Keyword(s):  
Skeletal Muscle ◽  
Small Intestine ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Mouse Tissues

Three preparations of radioactive yeast nucleic acids were fed to mice. One was labeled predominantly in the guanine moiety, one was labeled predominantly in the adenine moiety, and in one adenine and guanine were labeled equally. Most of the nucleic acid purines produced by digestion were excreted in the urine. However, a small amount was utilized for nucleotide and nucleic acid synthesis in the mouse tissues. Small intestine, liver and skeletal muscle contained most of the purines that were retained in the tissues. Dietary nucleic acid adenine appeared to be utilized somewhat more efficiently than was dietary nucleic acid guanine.

scholarly journals Antimicrobial Drug Interactions: Systematic Evaluation of Protein and Nucleic Acid Synthesis Inhibitors

Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 114 ◽  
Author(s):  
Yilancioglu
Keyword(s):  
Protein Synthesis ◽  
Multidrug Resistance ◽  
Nucleic Acid ◽  
Drug Interactions ◽  
Treatment Options ◽  
Acid Synthesis ◽  
Mechanisms Of Action ◽  
Nucleic Acid Synthesis ◽  
Protein Synthesis Inhibitors ◽  
Nucleic Acid Synthesis Inhibitors

Antimicrobial multidrug resistance and its transmission among strains are serious problems. Success rate is decreased and treatment options are narrowed due to increasing bacterial multidrug resistance. On the other hand, the need for long-term efforts to discover new antibiotics and difficulties finding new treatment protocols make this problem more complex. Combination therapy, especially with synergistic use of antimicrobials is a rational treatment option with huge benefits. Thus, screening antibiotic interactions is crucial for finding better treatment options. Clinicians currently use combinatorial antibiotic treatment as an effective treatment option. However, antibiotics can show synergistic or antagonistic interactions when used together. In our study, we aimed to investigate interactions of antibiotics with different mechanisms of action. Antibiotics, which act as protein synthesis inhibitors (P) and nucleic acid synthesis inhibitors (N) were used in our study. We tested 66 (PN), 15 (NN), and 55 (PP) drug pairs on the Escherichia coli strain. The Loewe additivity model was used and alpha scores were calculated for analysis of interactions of drug combinations. Drug interactions were categorized as synergistic or antagonistic. Accordingly, pairwise combinations of protein synthesis inhibitors (PP) showed stronger synergistic interactions than those of nucleic acid synthesis inhibitors (NN) and nucleic acid synthesis–protein synthesis inhibitors (PN). As a result, the importance of mechanisms of action of drugs is emphasized in the selection of synergistic drug combinations.

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