Effects of Intraventricular Injections of N6,O2′-Dibutyryl Adenosine 3′,5′-Monophosphate on the Rat Brain Cholinergic System

1975 ◽  
Vol 53 (5) ◽  
pp. 634-635 ◽  
Author(s):  
William E. Askew ◽  
Beng T. Ho
Keyword(s):  
Cerebral Cortex ◽  
Cyclic Amp ◽  
Choline Acetyltransferase ◽  
Cholinergic System ◽  
Acetylcholinesterase Activity ◽  
Dibutyryl Cyclic Amp ◽  
Subcortical Region ◽  
Intraventricular Injections ◽  
Subcortical Regions ◽  
Choline Acetyltransferase Activity

Intraventricular injections of dibutyryl cyclic AMP effected increases of acetylcholine in the cerebral cortex – striatal and subcortical regions. No increase in choline acetyltransferase activity was observed; however, there was a significant decrease in acetylcholinesterase activity in the subcortical region.

scholarly journals Some components of the cholinergic system in the prawn Palaemonetes varians (Leach)

1973 ◽  
Vol 135 (4) ◽  
pp. 673-682 ◽  
Author(s):  
Robin D. Veldsema-Currie
Keyword(s):  
High Activity ◽  
Choline Acetyltransferase ◽  
Cholinergic System ◽  
Nervous Tissue ◽  
Acetylcholinesterase Activity ◽  
Abdominal Muscles ◽  
Suboesophageal Ganglion ◽  
Isoelectric Points ◽  
Stomatogastric System ◽  
Choline Acetyltransferase Activity

1. An enzyme similar to mammalian acetylcholinesterase is found in high activity in the nervous tissue of Palaemonetes varians, i.e. eyes plus stalks, brain, suboesophageal ganglion and ventral cord. Acetylcholinesterase is also found associated with the abdominal muscles. Multiple enzyme forms are found in extracts of nervous tissues and muscles by electrophoresis and isoelectric focusing. 2. Cholinesterase is present in high activity in the stomatogastric system of P. varians. Three electrophoretically separable forms are found, having isoelectric points at pH4.2, 4.5 and 5.4. 3. Approx. 50% of the total acetylcholinesterase activity, approx. 80% of the choline acetyltransferase activity and 100% of the acetylcholine equivalents are found associated with the nervous tissue. Among the tissues examined, eyes plus stalks were the richest source of both choline acetyltransferase and acetylcholine equivalents. Suboesophageal ganglion and brain also contained large amounts of these components. 4. The distribution of these components could support the function of acetylcholine as a central and/or sensory transmitter in P. varians.

Neurotensin immunoreactivity and choline acetyltransferase activity in the cerebral cortex in Parkinson's disease

1983 ◽  
Vol 7 (3) ◽  
pp. 277 ◽  
Author(s):  
J Alan Biggins ◽  
Robert H Perry ◽  
John M Candy ◽  
Elaine K Perry ◽  
Clive A Bloxham ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cerebral Cortex ◽  
Choline Acetyltransferase ◽  
Acetyltransferase Activity ◽  
Choline Acetyltransferase Activity

AN UNEVEN DISTRIBUTION OF CHOLINE ACETYLTRANSFERASE ACTIVITY IN THE PITUITARY NEUROINTERMEDIATE LOBE OF THE RAT

1980 ◽  
Vol 85 (3) ◽  
pp. 497-501 ◽  
Author(s):  
MARIA G. P. GALLARDO ◽  
M. A. CANNATA ◽  
J. H. TRAMEZZANI
Keyword(s):  
Pituitary Gland ◽  
Choline Acetyltransferase ◽  
Cholinergic System ◽  
Male Rats ◽  
Uneven Distribution ◽  
Pars Intermedia ◽  
Junction Area ◽  
Acetyltransferase Activity ◽  
Neurointermediate Lobe ◽  
Choline Acetyltransferase Activity

The neurointermediate lobe of the pituitary gland of male rats was assayed for choline acetyltransferase (CAT) activity. Precise microsample punches were obtained from neurohypophysial tissue, pure pars intermedia tissue and from the junction area between them. The level of CAT activity (pmol/h per μg protein) in the neurohypophysis, pars intermedia and junction area were 0·390 ± 0·038 (s.e.m.), 0·228 ± 0·042 and 1·824 ± 0·268 respectively. These values show an uneven distribution of CAT in the neurointermediate lobe. The hypothesis of a cholinergic system located in the junction area has been advanced.

scholarly journals Regional distributions of choline acetyltransferase and acetylcholinesterase activities in layers of rat cerebellar vermis.

1983 ◽  
Vol 31 (7) ◽  
pp. 927-937 ◽  
Author(s):  
C D Ross ◽  
J T Smith ◽  
D A Godfrey
Keyword(s):  
Choline Acetyltransferase ◽  
Granular Layer ◽  
Molecular Layer ◽  
Mossy Fibers ◽  
Acetylcholinesterase Activity ◽  
Cerebellar Vermis ◽  
Numerical Density ◽  
Acetyltransferase Activity ◽  
Acetylcholinesterase Staining ◽  
Choline Acetyltransferase Activity

Entire sagittal sections of rat cerebellar vermis were dissected into microgram-sized samples of molecular, granular, and white matter layers. Assayed activities of choline acetyltransferase and acetylcholinesterase were plotted back onto sectional maps of exact sample locations. On the average, the activities of choline acetyltransferase and acetylcholinesterase in the granular layer were about four and five times, respectively, those in the molecular layer. The highest activity of both enzymes was in the granular layer of the vestibulocerebellum, the nodulus and ventral uvula (lobules X and IXc of Larsell). This activity might be related to the secondary vestibulocerebellar projection, terminating as mossy fibers in the granular layer of this region. Intermediate levels of activity were found in the granular layer of the dorsal uvula (lobule IX, a and b). The lowest activities of both enzymes in the granular layer were in the culmen (lobule V). A 7.1-fold difference in choline acetyltransferase activity and a 4.5-fold difference in acetylcholinesterase activity were found between the granular layer of lobules V and X. The numerical density of aggregates of acetylcholinesterase staining product in the granular layer correlated much better with assayed acetylcholinesterase activity than with choline acetyltransferase activity.

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