Ribonucleic Acid Polymerase Induced in L-Cells Infected with Vesicular Stomatitis Virus

1973 ◽  
Vol 51 (5) ◽  
pp. 721-729 ◽  
Author(s):  
Helene Galet ◽  
Joseph G. Shedlarski Jr. ◽  
Ludvik Prevec
Keyword(s):  
Enzyme Activity ◽  
Rna Polymerase ◽  
Vesicular Stomatitis Virus ◽  
Ribonucleic Acid ◽  
Polymerase Activity ◽  
L Cells ◽  
Ribonucleoprotein Complexes ◽  
Rna Polymerase Activity ◽  
Vesicular Stomatitis ◽  
Long Chain

RNA polymerase activity capable of synthesizing long-chain, heteropolymeric RNA is associated with specific ribonucleoprotein complexes present in the cytoplasm of cells infected with vesicular stomatitis virus (VSV). The synthesis is independent of added exogenous template and the product labelled with GTP precursors can be totally annealed with excess virion RNA. The enzyme activity is therefore a transcriptase similar to that observed in association with the mature VSV virion.

RNA polymerase activity and poly(A) synthesizing activity in defective T particles of vesicular stomatitis virus

Virology ◽  
1974 ◽  
Vol 58 (1) ◽  
pp. 240-249 ◽  
Author(s):  
M.E. Reichmann ◽  
L.P. Villarreal ◽  
D. Kohne ◽  
J. Lesnaw ◽  
J.J. Holland
Keyword(s):  
Rna Polymerase ◽  
Vesicular Stomatitis Virus ◽  
Polymerase Activity ◽  
Rna Polymerase Activity ◽  
Vesicular Stomatitis

scholarly journals Ribonucleic acid polymerase activities in Jerusalem-artichoke tissue

1974 ◽  
Vol 143 (1) ◽  
pp. 107-113 ◽  
Author(s):  
John R. Gore ◽  
John Ingle
Keyword(s):  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Deae Cellulose ◽  
Jerusalem Artichoke ◽  
Polymerase Activity ◽  
Tuber Tissue ◽  
Rna Polymerase Activity ◽  
Rna Accumulation ◽  
The Relationship

1. Artichoke tuber tissue contained RNA polymerase activity bound to the chromatin and in the supernatant after chromatin sedimentation. 2. The activity in the supernatant, the soluble polymerase, was fractionated into polymerases I and II by DEAE-cellulose chromatography, and the properties of each activity were determined. 3. The proportions of chromatin-bound and soluble activities varied with growth of the tissue, and there was a correlation between chromatin-bound activity and RNA accumulation. 4. The properties of the solubilized chromatin activity were compared with those of the soluble activity, and the relationship between these two activities is discussed.

Studies on Isolated Brain Nuclear DNA-Dependent RNA Polymerase

1972 ◽  
Vol 50 (3) ◽  
pp. 299-304 ◽  
Author(s):  
Vijendra K. Singh ◽  
S. C. Sung
Keyword(s):  
Enzyme Activity ◽  
Rna Polymerase ◽  
Actinomycin D ◽  
Nuclear Dna ◽  
Divalent Cations ◽  
Polymerase Activity ◽  
Maximum Activity ◽  
Optimum Concentration ◽  
Brain Nuclei ◽  
Rna Polymerase Activity

DNA-dependent RNA polymerase, which was isolated from beef brain nuclei, was stimulated by Mn2+ and Mg2+. The polymerase was about four times as active with Mn2+ as with Mg2+ but both of these divalent cations were required for maximum activity. KCl stimulated the enzyme activity with an optimum concentration around 0.2 M. The stimulation by KCl was much more pronounced in the presence of Mn2+ or Mn2+ plus Mg24 than in the presence of Mg2+ alone. Spermidine and spermine also stimulated the RNA polymerase activity and this stimulation was much greater in the presence of Mn2+ than in the presence of Mg2+ or both together. With Mn2+ plus Mg2+, spermidine had little or no stimulatory effect when heat-denatured DNA served as template, whereas with Mn2+ alone, spermidine markedly stimulated the enzyme in the presence of either native or heat-denatured DNA. The effect of spermidine was different with Mg2+ alone. The enzyme was inhibited as much as 75% by actinomycin D and almost completely by α-amanitin but not by rifampicin. Yeast RNA inhibited the enzyme activity considerably while spermidine appeared to overcome and prevent this inhibition of RNA polymerase.

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