Novel ribonucleic acid species inEimeria nieschulzi are associated with RNA-dependent RNA polymerase activity

1991 ◽  
Vol 77 (7) ◽  
pp. 581-584 ◽  
Author(s):  
T. Sepp ◽  
R. Entzeroth ◽  
J. Mertsching ◽  
P. H. Hofschneider ◽  
R. Kandolf
Keyword(s):  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Polymerase Activity ◽  
Rna Dependent Rna Polymerase ◽  
Rna Polymerase Activity

scholarly journals Thermolabile L-A virus-like particles from pet18 mutants of Saccharomyces cerevisiae.

1986 ◽  
Vol 6 (2) ◽  
pp. 404-410 ◽  
Author(s):  
T Fujimura ◽  
R B Wickner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Polymerase Activity ◽  
Nonpermissive Temperature ◽  
Wild Type ◽  
Rna Dependent Rna Polymerase ◽  
Virus Like Particles ◽  
Rna Polymerase Activity ◽  
Lines Of Evidence

pet18 mutations in Saccharomyces cerevisiae confer on the cell the inability to maintain either L-A or M double-stranded RNAs (dsRNAs) at the nonpermissive temperature. In in vitro experiments, we examined the effects of pet18 mutations on the RNA-dependent RNA polymerase activity associated with virus-like particles (VLPs). pet18 mutations caused thermolabile RNA polymerase activity of L-A VLPs, and this thermolability was found to be due to the instability of the L-A VLP structure. The pet18 mutations did not affect RNA polymerase activity of M VLPs. Furthermore, the temperature sensitivity of wild-type L-A RNA polymerase differed substantially from that of M RNA polymerase. From these results, and from other genetic and biochemical lines of evidence which suggest that replication of M dsRNA requires the presence of L-A dsRNA, we propose that the primary effect of the pet18 mutation is on the L-A VLP structure and that the inability of pet18 mutants to maintain M dsRNA comes from the loss of L-A dsRNA.

An Assay for RNA-Dependent RNA Polymerase Activity

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4322
Author(s):  
Luyun Huang ◽  
John Gledhill ◽  
Craig E. Cameron
Keyword(s):  
Rna Polymerase ◽  
Polymerase Activity ◽  
Rna Dependent Rna Polymerase ◽  
Rna Polymerase Activity

scholarly journals Ribonucleic acid polymerase activities in Jerusalem-artichoke tissue

1974 ◽  
Vol 143 (1) ◽  
pp. 107-113 ◽  
Author(s):  
John R. Gore ◽  
John Ingle
Keyword(s):  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Deae Cellulose ◽  
Jerusalem Artichoke ◽  
Polymerase Activity ◽  
Tuber Tissue ◽  
Rna Polymerase Activity ◽  
Rna Accumulation ◽  
The Relationship

1. Artichoke tuber tissue contained RNA polymerase activity bound to the chromatin and in the supernatant after chromatin sedimentation. 2. The activity in the supernatant, the soluble polymerase, was fractionated into polymerases I and II by DEAE-cellulose chromatography, and the properties of each activity were determined. 3. The proportions of chromatin-bound and soluble activities varied with growth of the tissue, and there was a correlation between chromatin-bound activity and RNA accumulation. 4. The properties of the solubilized chromatin activity were compared with those of the soluble activity, and the relationship between these two activities is discussed.

scholarly journals High-throughput identification of compounds targeting influenza RNA-dependent RNA polymerase activity

2010 ◽  
Vol 107 (45) ◽  
pp. 19151-19156 ◽  
Author(s):  
C.-Y. Su ◽  
T.-J. R. Cheng ◽  
M.-I. Lin ◽  
S.-Y. Wang ◽  
W.-I. Huang ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
High Throughput ◽  
Polymerase Activity ◽  
Rna Dependent Rna Polymerase ◽  
Rna Polymerase Activity
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