Alkaline and Acid Ribonucleases of Kidney and Spleen of Nephrotic Rats

1971 ◽  
Vol 49 (5) ◽  
pp. 535-542 ◽  
Author(s):  
D. M. Nicholls ◽  
Edward S. Bishay
Keyword(s):  
Ribonuclease Activity ◽  
Ribonuclease Inhibitor ◽  
Inhibitor Activity ◽  
Alkaline Ribonuclease

Alkaline ribonuclease activity (pH 7.8) was markedly decreased in all the fractions of kidney homogenates from nephrotic rats compared to the fractions of kidney homogenates from control rats. In contrast to the active alkaline ribonuclease activity at pH 7.8, the latent alkaline ribonuclease activity at pH 7.8 was increased in fractions from homogenates of nephrotic kidney due to increased ribonuclease inhibitor activity. In liver from nephrotic rats, however, there was no decrease in pH 7.8 alkaline ribonuclease activity and no increase in alkaline ribonuclease inhibitor. Alkaline ribonuclease activity at pH 9.5 and acid ribonuclease activity (pH 5.8 and 4.8) were also decreased in fractions from homogenates of kidney from nephrotic rats but not to the same degree as pH 7.8 alkaline ribonuclease activity. In fractions from homogenates of spleen, only small changes in ribonuclease activity were noted in nephrotic rat preparations compared to control rat preparations.

Effect of age, weight and adequacy of zinc intake on the balance between alkaline ribonuclease and ribonuclease inhibitor in various tissues of the rat

1978 ◽  
Vol 39 (2) ◽  
pp. 375-382 ◽  
Author(s):  
J. K. Chesters ◽  
Marie Will
Keyword(s):  
Ribonuclease Activity ◽  
Zn Deficiency ◽  
Ribonuclease Inhibitor ◽  
Zinc Intake ◽  
Zn Concentration ◽  
Tissue Homogenates ◽  
Alkaline Ribonuclease ◽  
Natural Inhibitor ◽  
Effect Of Age

1. Deficiency of zinc inhibits growth and also increases the activity of alkaline ribonuclease in certain tissues of the rat (Prasad & Oberleas, 1973). Zn could influence ribonuclease activity by direct effects on the enzyme or its natural inhibitor, or non-specifically as occurs when growth rate is affected by various other factors. These possibilities were studied.2. Alkaline ribonuclease was shown to be inhibited by Zn in vitro, but the concentrations of Zn required were so high that the enzyme was probably not directly affected by changes in tissue Zn concentration caused by dietary deficiency.3. At lower concentrations, Zn added in vitro increased the activity of alkaline ribonuclease in tissue homogenates probably by inactivating the inhibitor of the enzyme.4. Age, weight and particularly food restriction caused tissue-specific alterations of ribonuclease and ribonuclease inhibitor concentrations in liver, kidney, oesophagus, testis and thymus.5. The ribonuclease activities in liver, kidney and testis of Zn-deficient rats were unaltered in comparison with those of pair-fed rats. In thymus, which decreased in weight in the Zn-deficient animals, there was a concomitant increase in ribonuclease activity, but in oesophagus, the deficiency reduced the activity of ribonuclease.6. The effects of Zn deficiency upon alkaline ribonuclease and its inhibitor are probably secondary consequences of reductions in food intake or growth.

Alkaline Ribonuclease Activity in Maternal Serum and in Serum of Newborns from Birth up to Thirty Days of Age

Neonatology ◽  
1985 ◽  
Vol 47 (4) ◽  
pp. 211-216
Author(s):  
Cleide Enoir Petean Trindade ◽  
Fernando José de Nóbrega ◽  
Maria Eneida Aiello Sartor ◽  
Cleide Yochie Suguihara ◽  
Suzana Souza Queiroz Tonete ◽  
...  
Keyword(s):  
Maternal Serum ◽  
Ribonuclease Activity ◽  
Alkaline Ribonuclease

Amniotic fluid alkaline ribonuclease activity: an indicator of foetal growth retardation and anencephaly

1984 ◽  
Vol 143 (2) ◽  
pp. 157-162 ◽  
Author(s):  
Paul K. Buamah ◽  
Paul H. Scott ◽  
Andrew W. Skillen ◽  
Peter Taylor ◽  
A. Milford-Ward
Keyword(s):  
Amniotic Fluid ◽  
Growth Retardation ◽  
Ribonuclease Activity ◽  
Foetal Growth ◽  
Alkaline Ribonuclease

Effect of detergents upon ribonuclease inhibitor activity

Life Sciences ◽  
1971 ◽  
Vol 10 (23) ◽  
pp. 1321-1326 ◽  
Author(s):  
Ljuben M. Sirakov ◽  
A.V. Schulga
Keyword(s):  
Ribonuclease Inhibitor ◽  
Inhibitor Activity
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