Ion exchange chromatography of 2-amino-2-deoxy-D-hexoses

1970 ◽  
Vol 48 (3) ◽  
pp. 386-388 ◽  
Author(s):  
M. Yaguchi ◽  
M. B. Perry
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Citrate Buffer ◽  
Neutral Amino Acids ◽  
Amino Acid Analyzer

An amino acid analyzer is used to separate seven 2-ammo-2-deoxy-D-hexoses (allosamine, altrosamine, glucosamine, mannosamine, gulosamine, galactosamine, and talosamine). A borate citrate buffer at pH 7.24 is used at 60 °C. Acidic and neutral amino acids, if present, are eluted before any of the hexosamines.

Effect of Deproteinating Agents (Picric Acid and Sulfosalicylic Acid) on Analysis for Free Amino Acids in Swine Blood and Tissue

1969 ◽  
Vol 52 (5) ◽  
pp. 981-984 ◽  
Author(s):  
J E Knipfel ◽  
D A Christensen ◽  
B D Owen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino ◽  
Biological Fluids ◽  
Picric Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Sulfosalicylic Acid ◽  
Coefficients Of Variation

Abstract Amino acid analyses were performed on samples of blood, liver tissue, loin muscle, and ham muscle by ion exchange chromatography after deproteination of the samples with picric acid or sulfosalicylic acid (SSA). Resolution of threonine and serine from the ion exchange column was poor when SSA was used as the deproteinating agent. Twelve of sixteen amino acids were higher (P < 0.05) in serum deproteinated with picric acid as compared to concentrations determined after SSA deproteination. Amino acid values for ham muscle tended to be higher after deproteination with picric acid; however, with liver and loin muscle samples, the values were somewhat higher after SSA deproteination. In both serum and tissue analyses, coefficients of variation were lower for niGSt amino acids when picric acid was utilized as the deproteinating agent. The latter observation, in particular, suggests that picric acid is preferable to SSA as a deproteinating agent before amino acid analyses of biological fluids. Standardization of methods of deproteination is needed to allow meaningful comparisons of data.

scholarly journals Impairments of placental amino acid metabolism in fetal growth restriction

2017 ◽  
Vol 63 (3) ◽  
pp. 266-271 ◽  
Author(s):  
T.N. Pogorelova ◽  
V.O. Gunko ◽  
V.V. Avrutskaya ◽  
L.V. Kaushanskaya ◽  
O.A. Durnitsyna
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Growth Restriction

The content of the amino acids in the placenta during physiological pregnancy and fetal growth restriction (FGR) has been investigated my means of the method of ion-exchange chromatography. It has been found that in FGR the placental amino acid pool is characterized by a decreased content of arginine, proline, alanine, serine, cysteine, methionine, tryptophan, leucine, threonine, tyrosine, phenylalanine, glutamine and an increased content of dicarboxylic amino acids, lysine, histidine and glycine. These changes are accompanied by altered activity of some enzymes of amino acid metabolism, and the degree of these changes correlates with the level of corresponding amino acids.

scholarly journals Amino Acid Accumulation by Human Reticulocytes

Blood ◽  
1960 ◽  
Vol 16 (5) ◽  
pp. 1564-1571 ◽  
Author(s):  
DAVID W. ALLEN
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Concentration Ratio ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Red Cells ◽  
Red Cell ◽  
Acid Accumulation ◽  
Amino Acid Accumulation

Abstract 1. The concentrations of certain amino acids were measured by ion exchange chromatography in mature red cells, in red cells with a high percentage of reticulocytes, and in the plasma in which these red cells had been suspended. The concentration ratio as a measure of net accumulation of the red cells for amino acids was derived from these data. 2. Adult red cells can accumulate glycine and alanine, but not methionine, valine, isoleucine or leucine. 3. Reticulocytes can accumulate glycine best, then alanine, then methionine, isoleucine, and leucine to approximately the same extent. They were unable to maintain a concentration gradient for valine. 4. In two patients with thalassemia, no abnormality was found in the plasma or red cell levels of citrulline, proline, glycine, alanine, valine, isoleucine, tyrosine, or phenylalanine. In one of the thalassemic patients no abnormality of histidine was found. The amino acid accumulation by thalassemic, or fetal red cells was the same as for normal red cells of comparable reticulocyte percentage.

AMINO ACID COMPOSITION OF UREDOSPORES OF PUCCINIA GRAMINIS TRITICI

1965 ◽  
Vol 43 (6) ◽  
pp. 695-699 ◽  
Author(s):  
David Stefanye ◽  
Kenneth R. Bromfield
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Spore Wall ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Puccinia Graminis ◽  
Total Free Amino Acids

Uredospores of Puccinia graminis var. tritici (race 56) were analyzed quantitatively for total free amino acids and ninhydrin-positive substances by ion-exchange chromatography. Extracts of these substances were obtained by leaching the spores and by re-extracting leached spores with boiling water. Thirty-five ninhydrin-positive compounds were found and identified. The leach extract differed quantitatively from the extract obtained by boiling although both contained the same 35 substances. It is proposed that there are easily extractable ninhydrin-positive substances coating the spore wall and ninhydrin-positive substances in the protoplasm that can be extracted only with difficulty.

Isolation and structure determination of the peptides from the chymotryptic hydrolysate of the alkaline protease from Aspergillus flavus

1980 ◽  
Vol 45 (7) ◽  
pp. 1996-2028 ◽  
Author(s):  
Otakar Mikeš ◽  
Jaroslava Turková ◽  
Geoffrey Allen ◽  
Nguyen bao Toan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Aspergillus Flavus ◽  
Alkaline Protease ◽  
Paper Electrophoresis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Trichloracetic Acid

From the chymotryptic hydrolysate of 1.08 g of the precipitate obtained on treatment of alkaline protease from Aspergillus flavus with trichloracetic acid 134 peptides were isolated by means of ion exchange chromatography, paper electrophoresis and paper chromatography. Among these 38 peptides containing 311 amino acids were isolated in amounts exceeding 0.50μmol. The peptides were characterized by amino acid analysis, electric charge and also mostly by the terminal groups determination. In the case of peptides isolated in larger amounts the complete or at least the partial sequence of amino acids has been determined. In all peptides the total isolated amount in μmol was determined. The peptides containing basic amino acids were subfractionated with trypsin.

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