A computer program for writing the steady-state rate equation for a multisubstrate enzymic reaction

1969 ◽  
Vol 47 (10) ◽  
pp. 941-944 ◽  
Author(s):  
R. O. Hurst
Keyword(s):  
Steady State ◽  
Computer Program ◽  
Rate Equation ◽  
Digital Computer ◽  
Memory Capacity ◽  
Enzymic Reaction ◽  
Steady State Rate ◽  
State Rate ◽  
Enzyme Species

The determinant procedure for deriving the rate equation for an enzymic mechanism has been converted into Fortran IV language for use with a digital computer. The distribution equations for three to nine enzyme species, which may interact along all possible pathways, can be determined readily, limited only by the size of the memory capacity of the computer employed. The method permits several mechanisms to be analyzed in a matter of a few minutes.

scholarly journals Studies on the unusual behaviour of bovine liver UDP-glucose dehydrogenase in assays at acid and neutral pH and on the presence of tightly bound nucleotide material in purified preparations of this enzyme

1988 ◽  
Vol 255 (3) ◽  
pp. 775-780 ◽  
Author(s):  
F M Dickinson
Keyword(s):  
Steady State ◽  
Glucose Dehydrogenase ◽  
Bovine Liver ◽  
Enzyme Release ◽  
Unusual Behaviour ◽  
Ph Values ◽  
Steady State Rate ◽  
State Rate ◽  
Slow Dissociation ◽  
Enzyme Species

Assays of UDP-glucose dehydrogenase at pH 6.0 show long (10-15 min) lag periods before the steady-state rate is established, but at pH 9.0 no lag is observed. At intermediate pH values the lag is progressively shorter as the pH becomes more alkaline. The behaviour of the enzyme in assays at neutral and acid pH depends on the pH and concentration of the enzyme used to initiate the assay. The steady-state rate at pH 6.0 is strongly concentration-dependent. It is suggested that these phenomena arise because of the slow dissociation of an inactive enzyme species to an active one. Purified preparations of the enzyme release approx. 1 mol of a UDP-sugar/mol of enzyme subunit on denaturation. The identity of the UDP-sugar is unknown.

scholarly journals An automatic method for deriving steady-state rate equations

1977 ◽  
Vol 165 (1) ◽  
pp. 55-59 ◽  
Author(s):  
A Cornish-Bowden
Keyword(s):  
Steady State ◽  
Computer Program ◽  
Rate Equations ◽  
British Library ◽  
Automatic Method ◽  
Steady State Rate ◽  
State Rate

A method is described for systematically deriving steady-state rate equations. It is based on the schematic method of King & Altman [J. Phys. Chem. (1956) 60, 1375-1378], but is expressed in purely algebraic terms. It is suitable for implementation as a computer program, and a program has been written in FORTRAN IV and deposited as Supplementary Publication SUP 50078 (12 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1977) 161, 1-2.

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