Intracellular mechanisms for the decomposition of a lipid peroxide. I. Decomposition of a lipid peroxide by metal ions, heme compounds, and nucleophiles

1969 ◽  
Vol 47 (5) ◽  
pp. 485-492 ◽  
Author(s):  
P. J. O'Brien
Keyword(s):  
Catalytic Activity ◽  
Linoleic Acid ◽  
Metal Ions ◽  
Ph Dependence ◽  
Lipid Peroxide ◽  
Transition Metal Ions ◽  
Complexing Agents ◽  
Linoleic Acid Hydroperoxide ◽  
Hydrogen Donors ◽  
Acid Hydroperoxide

Linoleic acid hydroperoxide was prepared. Two types of mechanisms for its decomposition were found. The hydroperoxide was rapidly decomposed by certain transition metal ions, heme, and hemoprotein to a complex range of products, the decomposition being accompanied by changes in ultraviolet absorption spectra. The production of radical oxidizing species may account for these products. It was also found that the hydroperoxide could be decomposed by nucleophiles presumably in a nonradical reaction to a hydroxy acid without any change in ultraviolet spectra.The kinetics, the pH dependence, and the effects of metal-complexing agents, inhibitors, and hydrogen donors on the catalytic activity of the metal ions and heme compounds were also investigated.

Intracellular mechanisms for the decomposition of a lipid peroxide. II. Decomposition of a lipid peroxide by subcellular fractions

1969 ◽  
Vol 47 (5) ◽  
pp. 493-499 ◽  
Author(s):  
P. J. O'Brien ◽  
C. Little
Keyword(s):  
Linoleic Acid ◽  
Glutathione Peroxidase ◽  
Ph Dependence ◽  
Lipid Peroxide ◽  
Subcellular Fractions ◽  
Radical Mechanism ◽  
Complexing Agents ◽  
Linoleic Acid Hydroperoxide ◽  
Hydrogen Donors ◽  
Acid Hydroperoxide

The properties of subcellular fractions of rat liver in catalyzing the decomposition of linoleic acid hydroperoxide have been compared with those of transition salts, heme compounds, and nucleophiles. The properties compared included the range of products produced, the pH dependence of the reaction, and the effects of metal-complexing agents, inhibitors, and hydrogen donors. It was concluded that the decomposition of the hydroperoxide in the liver cell was due principally to reaction with the intracellular nucleophile glutathione by a mechanism catalyzed by the enzyme glutathione peroxidase. In the absence of glutathione, however, both the mitochondrial and microsomal fractions decomposed the hydroperoxide presumably by a radical mechanism probably involving the cytochromes.

scholarly journals Effects of Metal Ions and pH on the Stability of Linoleic Acid Hydroperoxide in the Water Phase

1997 ◽  
Vol 61 (12) ◽  
pp. 1973-1976 ◽  
Author(s):  
Tamako Nishiike ◽  
Satoko Kondo ◽  
Tamae Yamamoto ◽  
Aya Shigeeda ◽  
Yoshimi Yamamoto ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Metal Ions ◽  
Water Phase ◽  
Linoleic Acid Hydroperoxide ◽  
Acid Hydroperoxide ◽  
The Stability

Selenium-independent glutathione peroxidase activity in rabbit liver

1980 ◽  
Vol 58 (10) ◽  
pp. 1012-1017 ◽  
Author(s):  
Paul Morrissey ◽  
Peter J. O'Brien
Keyword(s):  
Linoleic Acid ◽  
Glutathione Peroxidase ◽  
Glutathione Transferase ◽  
Lipid Peroxide ◽  
Rabbit Liver ◽  
Linoleic Acid Hydroperoxide ◽  
Glutathione Peroxidase Activity ◽  
Liver Cytosol ◽  
Acid Hydroperoxide ◽  
Rat Liver Cytosol

The reduction of linoleic acid hydroperoxide catalyzed by rat liver cytosol was previously shown to be catalyzed by a selenium-dependent glutathione peroxidase. In contrast, the activity in rabbit liver cytosol could also be attributed to a selenium-independent glutathione peroxidase present in an approximately equal amount to the selenium-dependent peroxidase. The selenium-independent peroxidase copurified with glutathione transferase B and was completely inhibited by antitransferase B antiserum and transferase substrates. These results suggest that glutathione transferase B in rabbit liver cytosol is involved in the intracellular decomposition of lipid peroxide and could explain the lower selenium requirement of rabbits in comparison with other species.

The effect of partial exchange of sulphonated macroporous styrene-divinylbenzene copolymers for different ions on their catalytic activity

1981 ◽  
Vol 46 (10) ◽  
pp. 2354-2363 ◽  
Author(s):  
Svatomír Kmošták ◽  
Karel Setínek
Keyword(s):  
Catalytic Activity ◽  
Transition Metal ◽  
Metal Ions ◽  
Gas Phase ◽  
Transition Metal Ions ◽  
Exchange Capacity ◽  
Total Exchange Capacity ◽  
Styrene Divinylbenzene ◽  
Na Ions ◽  
Type Of Transition

The catalytic activity of sulphonated macroporous styrene-divinylbenzene copolymers, the exchange capacity of which was neutralized from 30, 50 and 80% by Fe(III) ions and from 30% by Na ions and that of Wofatit Y-37 ion exchanger neutralized from 10% of its total exchange capacity by several transition metal ions and by sodium has been studied in isomerisation of cyclohexene and dehydration of 1-propanol in the gas phase at 130 °C. It was demonstrated that in both reactions transition metal ions exhibit additional effect to the expected neutralization of the polymer acid groups. In the case of cyclohexene isomerization, this effect depends on the degree of crosslinking of polymer mass of the catalyst. Such dependence has not been, however, observed in dehydration of 1-propanol. The type of transition metal ions did not exhibit any significant effect on the catalytic activity of the polymer catalysts studied.

scholarly journals Interaction between Mitochondrial Cytochromes and Linoleic Acid Hydroperoxide

1982 ◽  
Vol 69 (6) ◽  
pp. 1308-1314 ◽  
Author(s):  
Jacques Dupont ◽  
Pierre Rustin ◽  
Claude Lance
Keyword(s):  
Linoleic Acid ◽  
Linoleic Acid Hydroperoxide ◽  
Acid Hydroperoxide ◽  
Mitochondrial Cytochromes

scholarly journals Flavonoids Suppress the Cytotoxicity of Linoleic Acid Hydroperoxide toward PC12 Cells

2002 ◽  
Vol 25 (8) ◽  
pp. 1093-1096 ◽  
Author(s):  
Naoko Sasaki ◽  
Takeshi Toda ◽  
Takao Kaneko ◽  
Naomichi Baba ◽  
Mitsuyoshi Matsuo
Keyword(s):  
Linoleic Acid ◽  
Pc12 Cells ◽  
Linoleic Acid Hydroperoxide ◽  
Acid Hydroperoxide
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