Effect of massive X-irradiation on proteins and protein synthesis in peanut cotyledons

1968 ◽  
Vol 46 (7) ◽  
pp. 685-689 ◽  
Author(s):  
R. B. van Huystee
Keyword(s):  
Gel Electrophoresis ◽  
De Novo ◽  
Protein Biosynthesis ◽  
Water Soluble ◽  
X Rays ◽  
Soluble Protein Content ◽  
De Novo Biosynthesis ◽  
Water Soluble Protein ◽  
Radiation Resistant ◽  
X Irradiation

Peanut seeds were irradiated with 500 kR of X rays and stored for 6 weeks. The cotyledons from 3-day-old seedlings were harvested, and incubated with leucine-14C. The water-soluble protein content of those cotyledons was half to one-third that of the non-irradiated cotyledons. Protein biosynthesis in the irradiated cotyledons was equally reduced to one-third or less of control. Separation of the extracted proteins by acrylamide-gel electrophoresis suggested that there may be a larger amount of small-molecular proteins in the irradiated system. De novo biosynthesis after irradiation was restricted in all protein fractions. Enzyme activity was also severely restricted. No particular radiation-resistant isoenzyme of peroxidase could be detected.

scholarly journals Effects of Different Heat Treatments on The Chemical and Microbiological Characteristics of Egg-Free and Quail Egg Dried Pasta

2018 ◽  
Vol 46 (2) ◽  
pp. 85-90
Author(s):  
Ildikó Szedljak ◽  
Viktória Tóth ◽  
Judit Tormási ◽  
Anikó Kovács ◽  
László Somogyi ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Antioxidant Capacity ◽  
Soluble Protein ◽  
Heat Treatments ◽  
Yellow Pigment ◽  
Water Soluble ◽  
Soluble Protein Content ◽  
Yellow Pigment Content ◽  
Microbiological Characteristics ◽  
Water Soluble Protein

Abstract In Hungary, dried pasta products are very popular amongst all groups of society. In recent years the demand for not only dried pasta made from hen eggs (Gallus gallus domesticus) but from alternative types of ingredients has increased. However, according to the literature the chemical and microbiological characteristics of this type of pasta have yet to be studied in depth. The effects of the use of quail eggs and heat treatments at different temperatures were studied by chemical and microbiological measurements. The activity of oxidative enzymes and nutritional characteristics (water-soluble total polyphenol content, water-soluble antioxidant capacity, peroxidase enzymatic activity, water-soluble protein content and yellow pigment content) was tested during our experiments. The data were evaluated by relevant statistical methods. Significant differences were found both between heat treatments and between the egg content of the dried pasta samples. The peroxidase enzymatic activity and yellow pigment content increased with temperature. However, the usage of quail eggs provides a higher water-soluble protein content and water-soluble antioxidant capacity. The presence of microorganisms is decreased by increasing the drying temperature. The number of all the examined microorganisms was within limits.

scholarly journals The Potential of Hydrolysate from Rabbit Meat Protein as an Angiotensin Converting Enzyme Inhibitor

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Edy Permadi ◽  
Jamhari Jamhari ◽  
Edi Suryanto ◽  
Zaenal Bachruddin ◽  
Yuny Erwanto
Keyword(s):  
Molecular Weight ◽  
Angiotensin Converting Enzyme ◽  
Soluble Protein ◽  
Ace Inhibitor ◽  
Water Soluble ◽  
Converting Enzyme ◽  
Soluble Protein Content ◽  
Water Soluble Protein ◽  
Rabbit Meat ◽  
Ace Inhibitory

This research aimed to investigate the rabbit meat hydrolysate potential as an angiotensin-converting enzyme (ACE) inhibitor. Indonesian local rabbit meats were used in this study. The research was conducted in Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, from August 2016 to February 2017. The local rabbit meats were hydrolyzed by pepsin, trypsin, and pancreatic. The obtained hydrolysates were then analyzed to identify the water-soluble protein content. The molecular weight of the hydrolysates were also confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The ACE inhibitory properties of the hydrolysates were analyzed in vitro. The results showed that pepsin, trypsin, and pancreatic hydrolysis showed a significant effect on the water-soluble protein content of rabbit meat (p<0.05). The water-soluble protein of rabbit meat hydrolysed by pepsin, trypsin, and pancreatic were 9.41, 7.66, and 9.75 mg/mL respectively. The molecular weight of the rabbit meat hydrolysate were increased from 10 to 43 kDa; 17 to 43 kDa; and 10 to 43 kDa, after hydrolysed by by pepsin, trypsin, and pancreatic respectively. Furthermore, the ACE inhibitory properties ) of the hydrolysed rabbit meat by pepsin, trypsin, and pancreatic were 439, 170, and 380 μg/mL, respectively. The rabbit meat hydrolysate showed a potential to be ACE inhibitor after hydrolyzed with pepsin, trypsin and pancreatic. Moreover, it also showed a promising potential to be used as bioactive components in different pharmaceutical applications. The highest ACE inhibitory capability was showed on trypsin hydrolysis with the total of 65.45% and 170 μg/mL ACE inhibition

scholarly journals Association of RYR1 and MYOG Genotype with Carcass and Meat Quality Traits in Grower-finisher Pigs

2010 ◽  
Vol 79 (2) ◽  
pp. 243-248
Author(s):  
Artur Rybarczyk ◽  
Arkadiusz Pietruszka ◽  
Eugenia Jacyno ◽  
Josef Dvořák ◽  
Tadeusz Karamucki ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Meat Quality ◽  
Water Soluble ◽  
Quality Traits ◽  
Soluble Protein Content ◽  
Large White ◽  
Meat Quality Traits ◽  
Water Soluble Protein ◽  
Water Holding ◽  
Intramuscular Fat Content

The study involved 110 hybrid grower-finisher pigs from Polish Large White x Polish Landrace sows sired by Pietrain breed boars and their crossbreds with the 990 line and Duroc. The aim of the study was to determine an association of the polymorphism of RYR1, myogenin (MYOG) genes and carcass meatiness level with carcass slaughter value and meat quality traits in hybrid pigs. Better meat quality was found in pigs of CC genotype at locus RYR1 than in those of CT genotype, with a similar carcass slaughter value and meat chemical composition. No significant differences were found in meat traits (carcass slaughter value) and quality between AA and AB genotypes at locus MYOG. Similarly, no significant differences were found between carcass meatiness ranges adopted in meat quality and its chemical composition, except for water holding capacity, which was higher in pigs with ⪬ 54% meatiness than in those with > 54%. Moreover, interaction was found between carcass meatiness range and RYR1 genotype in relation to backfat thickness and intramuscular fat content, as well as a connection between carcass meatiness range and MYOG genotype in relation to water-soluble protein content. The results indicate the need to continue selection work towards the elimination of allele T RYR1 gene’s in pedigree herds to improve meat quality in fatteners.

Eds Of Radioactive Particles By Self-Induced Fluorescence

1990 ◽  
Vol 48 (2) ◽  
pp. 238-239
Author(s):  
Gregory L. Finch ◽  
Richard G. Cuddihy
Keyword(s):  
Electron Beam Irradiation ◽  
Nuclear Reactor ◽  
X Rays ◽  
Reactor Accident ◽  
Internal Radiation ◽  
X Ray ◽  
External Sources ◽  
X Irradiation ◽  
Available Information ◽  
Individual Particles

The elemental composition of individual particles is commonly measured by using energydispersive spectroscopic microanalysis (EDS) of samples excited with electron beam irradiation. Similarly, several investigators have characterized particles by using external monochromatic X-irradiation rather than electrons. However, there is little available information describing measurements of particulate characteristic X rays produced not from external sources of radiation, but rather from internal radiation contained within the particle itself. Here, we describe the low-energy (< 20 KeV) characteristic X-ray spectra produced by internal radiation self-excitation of two general types of particulate samples; individual radioactive particles produced during the Chernobyl nuclear reactor accident and radioactive fused aluminosilicate particles (FAP). In addition, we compare these spectra with those generated by conventional EDS.Approximately thirty radioactive particle samples from the Chernobyl accident were on a sample of wood that was near the reactor when the accident occurred. Individual particles still on the wood were microdissected from the bulk matrix after bulk autoradiography.

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