DETERMINATION BY THIN-LAYER CHROMATOGRAPHY OF URINARY HOMOVANILLIC ACID IN NORMAL AND DISEASE STATES

1963 ◽  
Vol 41 (6) ◽  
pp. 1381-1388 ◽  
Author(s):  
Irwin Sankoff ◽  
T. L. Sourkes
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Homovanillic Acid ◽  
Hepatolenticular Degeneration ◽  
Huntington's Chorea ◽  
Disease States ◽  
Acetic Acid Water ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Ethyl Acetate Extracts

A rapid method using thin-layer chromatography has been developed to measure urinary HVA (homovanillic acid). Ethyl acetate extracts of urine are chromatographed on glass plates coated with silica gel. The solvent used is the organic phase of benzene: acetic acid: water (2:3:1) mixture. The eluted HVA is treated with Folin's phenol reagent and absorbance is measured at 750 mμ in a spectrophotometer. Normal amounts of HVA in human urine are 8.23 ± 2.96 (mean ± standard deviation) mg/24 hours or 6.42 ± 2.28 mg/g of creatinine. Higher values were obtained in cases of ganglioneuroma and neuroblastoma, in a case of pheochromocytoma, and in one of two cases of hepatolenticular degeneration (Wilson's disease). In three cases of Huntington's chorea, values lay in the normal range.

DETERMINATION BY THIN-LAYER CHROMATOGRAPHY OF URINARY HOMOVANILLIC ACID IN NORMAL AND DISEASE STATES

1963 ◽  
Vol 41 (1) ◽  
pp. 1381-1388 ◽  
Author(s):  
Irwin Sankoff ◽  
T. L. Sourkes
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Homovanillic Acid ◽  
Hepatolenticular Degeneration ◽  
Huntington's Chorea ◽  
Disease States ◽  
Acetic Acid Water ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Ethyl Acetate Extracts

A rapid method using thin-layer chromatography has been developed to measure urinary HVA (homovanillic acid). Ethyl acetate extracts of urine are chromatographed on glass plates coated with silica gel. The solvent used is the organic phase of benzene: acetic acid: water (2:3:1) mixture. The eluted HVA is treated with Folin's phenol reagent and absorbance is measured at 750 mμ in a spectrophotometer. Normal amounts of HVA in human urine are 8.23 ± 2.96 (mean ± standard deviation) mg/24 hours or 6.42 ± 2.28 mg/g of creatinine. Higher values were obtained in cases of ganglioneuroma and neuroblastoma, in a case of pheochromocytoma, and in one of two cases of hepatolenticular degeneration (Wilson's disease). In three cases of Huntington's chorea, values lay in the normal range.

Urinary Homogentisic Acid: Determination by Thin-Layer Chromatography

1973 ◽  
Vol 19 (5) ◽  
pp. 459-462 ◽  
Author(s):  
Jerome M Feldman ◽  
June Bowman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clinical Laboratory ◽  
Homogentisic Acid ◽  
Qualitative And Quantitative ◽  
Color Development ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Acid Determination

Abstract A new method is described for qualitative and quantitative determination of urinary homogentisic acid. The method involves extraction of urine with ethyl acetate, thin-layer chromatography of the extract on Silica Gel G, elution of the homogentisic acid into water, and color development with Folin’s phenol reagent. Absorbance is maximum at 750 nm and linear to a concentration of at least 5 mg of homogentisic acid per milliliter of urine. The method is highly specific for homogentisic acid; added gentisic acid, 3,4-dihydroxyphenylacetic acid, ascorbic acid, or L-3,4 dihydroxyphenylalanine do not interfere. The coefficient of variation "in-run" is 5.3%, "between-run" 6.8%. Using this method we have demonstrated a marked variation in day-to-day homogentisic acid excretion in a patient with alcaptonuria. This method, which offers some advantages over existing techniques, should be suitable for use in a clinical laboratory.

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA FLAVONOID DARI EKSTRAK ETANOL BUAH BELIMBING WULUH (Averrhoa bilimbi L.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS

2015 ◽  
Vol 1 (1) ◽  
pp. 22-34
Author(s):  
Siti Hamdanah ◽  
Syariful Anam ◽  
Jamaluddin Jamaluddin
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Shift Reagent ◽  
Flavonoid Compound ◽  
Polyphenolic Compound ◽  
Color Test ◽  
Oh Groups ◽  
Star Fruit ◽  
Acetic Acid Water ◽  
Layer Chromatography

Star fruits (Averrhoa bilimbi L.) from family of oxalidaceae is a plant which has potential as an antibacterial Staphylococcus aureus and Escherichia coli. Flavonoid are the active compounds from the extract of star fruit which has potential as an antibacterial. Star fruit were extracting by ethanol 96 % using maceration. Extract were identified using thin layer chromatography (TLC) with eluent analytic n-butanol- glacial acetic acid- water (6:1:3). Preparative isolation using thin layer chromatography resulting three bands. Each band was centrifuged to obtain isolates. Physics isolates were analyzing using spectrophotometer UV Vis and chemically using color test (shift reagent). UV Vis spectrum results with shift reagent from isolates 1, suspected as isoflavone compound with OH groups in position 6,7,4 and OH groups in O- ring A, in isolates 2 was not flavonoid compound and predicted as other polyphenolic compound and in isolates 3 suspected of flavone compound with OH groups in position 5,6,7 and OH groups in O-ring A

scholarly journals Profil Fitokimia Ekstrak Etil Asetat Temu Kunci (Boesenbergia rotunda L.) dan Serai (Cymbopogon citratus)

2021 ◽  
Vol 8 (1) ◽  
pp. 45
Author(s):  
Muhammad Priyadi ◽  
Nurul Chusna ◽  
Isnawati Isnawati ◽  
Opi Indriani
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Chemical Compounds ◽  
Cymbopogon Citratus ◽  
Color Reagent ◽  
Qualitative Test ◽  
Boesenbergia Rotunda ◽  
Layer Chromatography ◽  
Ethyl Acetate Extracts

Senyawa bahan alam yang terdapat pada tanaman memiliki banyak khasiat bagi kesehatan yang telah dibuktikan melalui pengobatan tradisional secara empiris. Identifikasi senyawa kimia sangat penting untuk mengetahui kemungkinan adanya senyawa yang dapat memiliki aktivitas farmakologi. Tanaman yang telah banyak digunakan oleh masyarakat termasuk pengobatan adalah temu kunci (Boesenbergia rotunda L.) dan serai (Cymbopogon citratus). Temu kunci dan serai diekstraksi menggunakan pelarut etil asetat. Uji fitokimia pada ekstrak etil asetat temu kunci dan serai dilakukan dengan uji kualitatif pereaksi warna dan pengendapan serta kromatografi lapis tipis untuk melihat gambaran pemisahan senyawa kimia yang terkandung. Ekstrak etil asetat temu kunci dan serai mengandung senyawa golongan alkaloid, terpenoid, flavonoid, fenol, dan kuinon. Senyawa pada temu kunci dan serai dapat dipisahkan menggunakan kromatografi lapis tipis dengan fase gerak etil asetat : n-heksan (8:2).    Kata Kunci: Fitokimia, Temu Kunci, Serai, Ekstrak Etil Asetat, Kromatografi Lapis Tipis Natural compounds found in plants have many health benefits that have been proven through empirically traditional medicine. Identification of chemical compounds is very important to determine the possibility of compounds having pharmacological activity. Plants that have been widely used by the community, including medicinal plants, are Temu Kunci (Boesenbergia rotunda L.) and serai or lemongrass (Cymbopogon citratus). Temu Kunci and lemongrass were extracted using ethyl acetate as a solvent. Phytochemical test on ethyl acetate extract of temu Kunci and lemongrass was carried out by qualitative test using color reagent and deposition and thin layer chromatography to see the description of the separation of the chemical compounds contained. Temu Kunci and lemongrass ethyl acetate extracts contain alkaloids, terpenoids, flavonoids, phenols, and quinones. Compounds in Temu Kunci and lemongrass can be separated using thin layer chromatography with ethyl acetate: n-hexane (8: 2) as mobile phase.

scholarly journals Simultaneous Determination of Bergenin and Gallic Acid in Bergenia ligulata Wall by High-Performance Thin-Layer Chromatography

2000 ◽  
Vol 83 (6) ◽  
pp. 1480-1483 ◽  
Author(s):  
Sateesh K Chauhan ◽  
Brijpal Singh ◽  
Sudhakar Agrawal
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Thin Layer Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Solvent System ◽  
Simultaneous Quantification ◽  
Acetic Acid Water ◽  
Layer Chromatography

Abstract A simple and reproducible high-performance thin-layer chromatographic method was developed for the simultaneous determination of bergenin and gallic acid in Bergenia ligulata. Water and methanol were used as the extracting solvents. The concentrations of bergenin and gallic acid in both of these solvents were found to be almost the same. The method involves separation of the components by thin-layer chromatography on a precoated Silica Gel 60 F254 plate with a solvent system of ethyl acetate–formic acid–acetic acid–water (100 + 11 + 11 + 27). The sensitivity of the method for bergenin was 0.30 μg, whereas for gallic acid it was 0.25 μg. The proposed method is precise and sensitive and can be used for the detection, monitoring, and simultaneous quantification of bergenin and gallic acid in B. ligulata.

scholarly journals Determination of Oxycodone Hydrochloride in Oral Solutions by High-Performance Thin-Layer Chromatography/Densitometry

2000 ◽  
Vol 83 (6) ◽  
pp. 1497-1501
Author(s):  
Hannele E M Salomies ◽  
Piia K Salo
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
High Performance ◽  
Acetic Acid Water ◽  
Oxycodone Hydrochloride ◽  
Layer Chromatography

Abstract A thin-layer chromatography/densitometry method was developed and validated for the determination of oxycodone hydrochloride in oral solutions by using silica gel plates. A horizontal technique was used for development of the plates. The optimum composition for the mobile phase, which provided a suitable Rf value of 0.6 for oxycodone, was propanol–acetic acid–water–25% ammonia–methanol (20 + 1 + 1 + 3 + 10). Detection was at 234 nm. Oxycodone hydrochloride was stable on the sorbent and was precisely and accurately measured in the range of 0.3–1.5 μg/band.

scholarly journals Thin Layer Chromatography (TLC) and Phytochemical Analysis of Moringa Oleifera Methanol, Ethanol, Water and Ethyl Acetate Extracts

2019 ◽  
Vol 05 (10) ◽  
pp. 817-820
Author(s):  
Ahlam Rashed Ameerah Shaeroun ◽  
Ahmed Belgasem Ahmed Hamed Alqamoudy ◽  
Khalifa.S Mohamed ◽  
Nadea Almunir Nouri Kushlaf ◽  
A.M.EL-mahmoudy Akram Almabrouk misbah ◽  
...  
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Moringa Oleifera ◽  
Phytochemical Analysis ◽  
Layer Chromatography ◽  
Ethyl Acetate Extracts
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