Thin-layer chromatography of urinary homovanillic acid and vanillylmandelic acid for large-scale neuroblastoma mass screening

1989 ◽  
Vol 17 (5-6) ◽  
pp. 364-367 ◽  
Author(s):  
Christiane Auray-Blais ◽  
Robert Giguére ◽  
Bernard Lemieux
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Screening ◽  
Large Scale ◽  
Homovanillic Acid ◽  
Vanillylmandelic Acid ◽  
Layer Chromatography

DETERMINATION BY THIN-LAYER CHROMATOGRAPHY OF URINARY HOMOVANILLIC ACID IN NORMAL AND DISEASE STATES

1963 ◽  
Vol 41 (1) ◽  
pp. 1381-1388 ◽  
Author(s):  
Irwin Sankoff ◽  
T. L. Sourkes
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Homovanillic Acid ◽  
Hepatolenticular Degeneration ◽  
Huntington's Chorea ◽  
Disease States ◽  
Acetic Acid Water ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Ethyl Acetate Extracts

A rapid method using thin-layer chromatography has been developed to measure urinary HVA (homovanillic acid). Ethyl acetate extracts of urine are chromatographed on glass plates coated with silica gel. The solvent used is the organic phase of benzene: acetic acid: water (2:3:1) mixture. The eluted HVA is treated with Folin's phenol reagent and absorbance is measured at 750 mμ in a spectrophotometer. Normal amounts of HVA in human urine are 8.23 ± 2.96 (mean ± standard deviation) mg/24 hours or 6.42 ± 2.28 mg/g of creatinine. Higher values were obtained in cases of ganglioneuroma and neuroblastoma, in a case of pheochromocytoma, and in one of two cases of hepatolenticular degeneration (Wilson's disease). In three cases of Huntington's chorea, values lay in the normal range.

Micro-analysis for quinidine in serum by thin-layer chromatography followed by fluorescence densitometry.

1983 ◽  
Vol 29 (12) ◽  
pp. 2100-2102 ◽  
Author(s):  
M Kelner ◽  
D N Bailey
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Large Scale ◽  
Internal Standard ◽  
Peak Height ◽  
Peak Height Ratio ◽  
Height Ratio ◽  
Coefficients Of Variation ◽  
Micro Analysis ◽  
Layer Chromatography

Abstract We report a thin-layer-chromatographic micro-analysis for quinidine in serum, with detection by fluorescence densitometry. Quinidine is extracted from 20 microL of serum at pH 13 into 3 mL of hexane/acetone solution (80/20 by vol) containing N-(1-naphthyl)ethylenediamine as internal standard. The extract is concentrated and applied to silica-gel-impregnated plates for conventional thin-layer chromatography. Quinidine is identified from its RF value and quantified from the peak-height ratio between quinidine and the internal standard, relative to that of simultaneously extracted serum standards. The proposed assay is sensitive (to 0.2 mg/L), specific for unmetabolized quinidine, precise (between-run coefficients of variation less than 6%), and readily adaptable to large-scale "batch" analysis. Peak-height ratio is linearly related to concentration to at least 20 mg/L. Quinidine concentrations in the serum of patients, as measured by the proposed method (x) and by a traditional double-extraction spectrofluorometric assay (y), were related as follows: y = 0.994x + 0.276 (r = 0.989, n = 20).

Determination of drugs in plasma by high-performance thin-layer chromatography.

1978 ◽  
Vol 24 (8) ◽  
pp. 1386-1392 ◽  
Author(s):  
D C Fenimore ◽  
C M Davis ◽  
C J Meyer
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Large Scale ◽  
Ultraviolet Spectrophotometry ◽  
Reflectance Spectrometry ◽  
Ultraviolet Reflectance ◽  
Layer Chromatography

Abstract High-performance thin-layer chromatography was used to determine chlorpromazine, amitriptyline, nortriptyline, imipramine, desipramine, phenobarbital, and phenytoin in plasma, to demonstrate the utility of this technique for routine analysis. We quantitated the separated components by use of ultraviolet reflectance spectrometry with detection limits as low as 1 microgram/liter. Regressions of psychoactive agents extracted from plasma were linear over the range of 0 to 300 microgram/liter. The anti-convulsant drugs, phenobarbital and phenytoin, were determined over a range of 0 to 50 mg/liter. Analyses were rapid, reproducible, and well-suited to large-scale programs. Separated components also can be identified in situ by ultraviolet spectrophotometry.

scholarly journals A Pharmacognostical and Phytochemical study of Sookshma Eladi Choornam

2020 ◽  
Vol 11 (4) ◽  
pp. 712-715
Author(s):  
Harinatha Chary B ◽  
Manu Rajagopal ◽  
Pavan Kumar S ◽  
Gnana Prasuna S
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Large Scale ◽  
Fine Powder ◽  
Chemical Study ◽  
Quality Parameters ◽  
Water Soluble ◽  
Dissolution Time ◽  
Phytochemical Study ◽  
Layer Chromatography

Introduction: Sookshma Eladi choornam is one of the Ayurvedic formulations specially mentioned for Kaphaja Hridroga in Hridroga prakarana of Bhaishajya Ratnavali.  Dhamani pratichaya (Atherosclerosis) is one of the diseases of Kaphaja nanatmaja vaydhi which is the more important cause for Ischemic Heart Disease. The cause for Atherosclerosis could be Hyperlipidemias. Eladi choornam is a compound preparation which contains powders of Sookshma ela (Elettaria cardamomum Maton.) and Pippali moolam (root of Piper longum L.,). As these drugs are Katu vipaka in nature they can be used in Hyerlipidemia.  To know the efficacy of the drugs their quality parameters are highly essential to manufacture in the large scale. Method: The present study deals with the Pharmacognostical and phyto-chemical study of Sookshma eladi choornam including Thin Layer chromatography study (TLC) as per the standard literature. Result: Consistency of Sookshma eladi choornam is fine powder. Colour was brown, odour was aromatic spicy smell, Taste was pungent, touch was smooth powder form,  Qualitative study showed that pH is 4.9, total ash value 12 %, loss on drying is 4.5%, Water soluble matter17%, Alcohol soluble matter 8%,  Acid insoluble ash 8.5%. Thin Layer chromatography (TLC) revealed one yellow spot.  Dissolution time is 4 minutes and Moisture content was 8%. Phytochemicals as Alkaloids, Glycosides, Tannins, Flavonoids and Phenols were found. Conclusion: Pharmacognostical, phyto-chemical and TLC studies inferred that the formulation meets the minimum quality standards. The study may be used as reference standard in the further quality control researches.

DETERMINATION BY THIN-LAYER CHROMATOGRAPHY OF URINARY HOMOVANILLIC ACID IN NORMAL AND DISEASE STATES

1963 ◽  
Vol 41 (6) ◽  
pp. 1381-1388 ◽  
Author(s):  
Irwin Sankoff ◽  
T. L. Sourkes
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Homovanillic Acid ◽  
Hepatolenticular Degeneration ◽  
Huntington's Chorea ◽  
Disease States ◽  
Acetic Acid Water ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Ethyl Acetate Extracts

A rapid method using thin-layer chromatography has been developed to measure urinary HVA (homovanillic acid). Ethyl acetate extracts of urine are chromatographed on glass plates coated with silica gel. The solvent used is the organic phase of benzene: acetic acid: water (2:3:1) mixture. The eluted HVA is treated with Folin's phenol reagent and absorbance is measured at 750 mμ in a spectrophotometer. Normal amounts of HVA in human urine are 8.23 ± 2.96 (mean ± standard deviation) mg/24 hours or 6.42 ± 2.28 mg/g of creatinine. Higher values were obtained in cases of ganglioneuroma and neuroblastoma, in a case of pheochromocytoma, and in one of two cases of hepatolenticular degeneration (Wilson's disease). In three cases of Huntington's chorea, values lay in the normal range.

TECHNIQUE FOR SEPARATION AND ESTIMATION OF ANDROSTERONE, AETIOCHOLANOLONE AND DEHYDROEPIANDROSTERONE BY USE OF THIN-LAYER CHROMATOGRAPHY

1966 ◽  
Vol 51 (3) ◽  
pp. 447-460 ◽  
Author(s):  
S. Sulimovici ◽  
B. Lunenfeld ◽  
M. C. Shelesnyak
Keyword(s):  
Spectral Analysis ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Large Scale ◽  
Quantitative Estimation ◽  
Small Samples ◽  
Gas Liquid Chromatography ◽  
Silyl Derivatives ◽  
Layer Chromatography

ABSTRACT A method is described for the quantitative estimation of urinary androsterone, aetiocholanolone and dehydroepiandrosterone which employs enzymatic hydrolysis for glucuronide conjugates, solvolysis for sulphate esters, ascending thin-layer chromatography, elution and spectrophotometric quantitation of the eluted steroids as Zimmermann chromogens. The reliability of the method was established by recovery experiments and by duplicate assays. Its specificity was confirmed by u. v. and i. r. spectral analysis of the urinary components eluted from the thin-layer chromatograms and by gas-liquid chromatography of these components and their trimethyl-silyl derivatives. Since the method is relatively rapid and requires small samples of urine it has the advantage of being suitable for large-scale clinical determinations.

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