SEPARATION OF BILE ACID CONJUGATES BY ION EXCHANGE CHROMATOGRAPHY

1963 ◽  
Vol 41 (1) ◽  
pp. 77-89 ◽  
Author(s):  
B. A. Gordon ◽  
A. Kuksis ◽  
J. M. R. Beveridge
Keyword(s):  
Bile Acid ◽  
Ion Exchange ◽  
Complete Separation ◽  
Exchange Chromatography ◽  
Ethanol Solution ◽  
Anion Exchange Column ◽  
Exchange Resins ◽  
Dowex 1 ◽  
Cholanic Acid ◽  
Acetate Form

A simple and reliable method for the separation of bile acid conjugates in quantities ranging from a few milligrams to several grams has been developed utilizing ion exchange resins. The bile acid conjugates were absorbed from an aqueous 25% ethanol solution on a Dowex-1 anion exchange column (acetate form). After the column was washed with aqueous 25% ethanol, the glycine conjugates were eluted with 0.05 N HCl in 25% ethanol and the taurine conjugates with 0.5 N HCl in 25% ethanol. The method permitted a complete resolution of the glycine and taurine conjugates. In addition, there was an effective though not complete separation of the tri- and di-hydroxy cholanic acid derivatives within each conjugate group. Using this method satisfactory fractionations have been performed on gallbladder bile from dog, ox, and man.

SEPARATION OF BILE ACID CONJUGATES BY ION EXCHANGE CHROMATOGRAPHY

1963 ◽  
Vol 41 (1) ◽  
pp. 77-89 ◽  
Author(s):  
B. A. Gordon ◽  
A. Kuksis ◽  
J. M. R. Beveridge
Keyword(s):  
Bile Acid ◽  
Ion Exchange ◽  
Complete Separation ◽  
Exchange Chromatography ◽  
Ethanol Solution ◽  
Anion Exchange Column ◽  
Exchange Resins ◽  
Dowex 1 ◽  
Cholanic Acid ◽  
Acetate Form

A simple and reliable method for the separation of bile acid conjugates in quantities ranging from a few milligrams to several grams has been developed utilizing ion exchange resins. The bile acid conjugates were absorbed from an aqueous 25% ethanol solution on a Dowex-1 anion exchange column (acetate form). After the column was washed with aqueous 25% ethanol, the glycine conjugates were eluted with 0.05 N HCl in 25% ethanol and the taurine conjugates with 0.5 N HCl in 25% ethanol. The method permitted a complete resolution of the glycine and taurine conjugates. In addition, there was an effective though not complete separation of the tri- and di-hydroxy cholanic acid derivatives within each conjugate group. Using this method satisfactory fractionations have been performed on gallbladder bile from dog, ox, and man.

Quantitation of o- and p-Sulfamoylbenzoic Acids in Commercial Saccharin by High-Performance Liquid Chromatography

1976 ◽  
Vol 59 (2) ◽  
pp. 243-250
Author(s):  
James J Nelson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Sodium Salt ◽  
Mobile Phase ◽  
High Performance ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Anion Exchange Column ◽  
Para Isomer

Abstract Quantitation of o- and p-sulfamoylbenzoic acid residues in saccharin and its sodium salt is achieved by a method comprising methanolic extraction and high-performance ion exchange chromatography. A commercially available anion exchange column was employed with an aqueous buffered (pH 9.2) mobile phase. As little as 80 ppm of the ortho-isomer and 25 ppm of the para-isomer can be accurately determined. The levels of detectability (2 times noise) are estimated as 8 ppm (0.16 μg on column) and 2.5 ppm (0.05 μg on column), respectively. Recoveries from saccharin ranged from 92.7 to 96.5% (ortho) and from 92.2 to 103.3% (para). Recoveries from the sodium salt ranged from 93.1 to 104.4% (ortho) and from 93.5 to 97.8% (para). Of 9 other potential saccharin impurities tested separately, only one was found to interfere slightly in the chromatographic part of the procedure.

Isolation and Fast Purification o f Neocarzinostatin by FPLC-Ion Exchange Chromatography

1983 ◽  
Vol 38 (11-12) ◽  
pp. 939-942
Keyword(s):  
Ion Exchange ◽  
Anion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
New Method ◽  
Clinical Grade ◽  
Anion Exchange Column ◽  
Exchange Column ◽  
Culture Filtrates ◽  
Reproducible Method

Neocarzinostatin, a highly toxic antitum or protein containing an essential nonprotein chromophore, can be isolated and purified from culture filtrates of Streptomyces carzinostaticus. Usually a lengthy procedure of up to 60 h is necessary for the isolation, including several chromatographic steps partly under conditions which favour inactivation of the drug by release of chromophore. We describe a new method yielding practically clinical grade Neocarzinostatin from crude extracts in 20 min. This very fast and reproducible method was made possible by using a Mono Q anion exchange column filled with monodisperse gel material which has been recently developed

scholarly journals Purified Haemoglobin Preparations in the Evaluation of HbA1c Determination by Ion Exchange Chromatography

1993 ◽  
Vol 30 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Margo P Cohen ◽  
Janice Witt ◽  
Van-Yu Wu
Keyword(s):  
Ion Exchange ◽  
Gel Electrophoresis ◽  
High Performance ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Clinical Samples ◽  
Ion Exchange Resins ◽  
Substantial Portion ◽  
Blood Samples ◽  
Exchange Resins

The use of ion exchange resins for the estimation of HbA1c in clinical samples rests on the assumption that HbA1c is effectively and efficiently separated from other N-terminally modified haemoglobins and from HbAo. To test this assumption, we applied highly purified preparations of HbA1a+1b, HbA1c and HbAo to ion exchange minicolumns, using conditions of application simulating actual blood samples and the first and second elution buffers provided by the manufacturer. The authenticity and purity of the applied haemoglobin preparations were documented by high performance liquid chromatography, gel electrophoresis and carbohydrate content. About 40% of the applied HbA1a+1b eluted in the first fraction; 45% eluted in the second fraction, and 10% to 15% required 1 mol/L NaCl to elute from the column. Of the applied HbA1c, 65–80% eluted where expected in the second fraction, about 20% required 1 mol/L NaCl to elute from the column, and the remainder eluted with HbA1a+1b. Some 3–6% of pure HbAo applied to minicolumns emerged in the second fraction, with the remainder eluting as expected after making the buffer 1 mol/L in NaCl. The results indicate that the fraction eluting from ion exchange minicolumn chromatography that is designated ‘HbA1c’ contains HbA1a+1b, and that a substantial portion of the HbA1c in an applied sample does not elute in this fraction.

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