THE EFFECTS OF ADRENAL CORTICAL EXTRACTS UPON CARBOHYDRATE METABOLISM WHEN MEDIUM COMPOSITION IS ALTERED

1955 ◽  
Vol 33 (5) ◽  
pp. 845-848
Author(s):  
D. W. Clarke
Keyword(s):  
Glucose Uptake ◽  
Glycogen Synthesis ◽  
Significant Inhibition ◽  
Medium Composition ◽  
Potassium Ions ◽  
Rat Diaphragm ◽  
Major Cation ◽  
Maximum Inhibition ◽  
Sodium And Potassium ◽  
Isolated Rat

The effects of adrenal cortical extract upon glucose uptake and upon glycogen synthesis in the isolated rat diaphragm were studied in three different media. The maximum inhibition by the extract upon the measured effects was seen in a medium which contained both sodium and potassium ions. In media which contained either of these alone as the major cation, there was no significant inhibition.

THE EFFECTS OF ADRENAL CORTICAL EXTRACTS UPON CARBOHYDRATE METABOLISM WHEN MEDIUM COMPOSITION IS ALTERED

1955 ◽  
Vol 33 (1) ◽  
pp. 845-848
Author(s):  
D. W. Clarke
Keyword(s):  
Glucose Uptake ◽  
Glycogen Synthesis ◽  
Significant Inhibition ◽  
Medium Composition ◽  
Potassium Ions ◽  
Rat Diaphragm ◽  
Major Cation ◽  
Maximum Inhibition ◽  
Sodium And Potassium ◽  
Isolated Rat

The effects of adrenal cortical extract upon glucose uptake and upon glycogen synthesis in the isolated rat diaphragm were studied in three different media. The maximum inhibition by the extract upon the measured effects was seen in a medium which contained both sodium and potassium ions. In media which contained either of these alone as the major cation, there was no significant inhibition.

THE INFLUENCE OF POTASSIUM ION UPON GLUCOSE UPTAKE AND GLYCOGEN SYNTHESIS IN THE ISOLATED RAT DIAPHRAGM

1955 ◽  
Vol 33 (1) ◽  
pp. 687-694 ◽  
Author(s):  
D. W. Clarke
Keyword(s):  
Glucose Uptake ◽  
Glycogen Synthesis ◽  
Potassium Ion ◽  
Ion Concentration ◽  
Rat Diaphragm ◽  
High Potassium ◽  
High Concentrations ◽  
Glycogen Breakdown ◽  
Isolated Rat

The amounts of glucose taken from a medium, and the amounts of glycogen synthesized, by rat hemidiaphragms were studied under various conditions. High concentrations of potassium ion inhibited the glucose uptake and there was also a reduced net glycogen synthesis. Glycogen breakdown was probably not increased by high potassium ion concentration. The effect of potassium was most marked when conditions were such that one would ordinarily expect a considerable glucose uptake or glycogen synthesis. The action of insulin was not peculiarly susceptible to potassium ion inhibition.

Serum factor in fasted rats inhibiting carbohydrate metabolism in the isolated diaphragm

1960 ◽  
Vol 198 (5) ◽  
pp. 1075-1078 ◽  
Author(s):  
E. R. Berman ◽  
E. Wertheimer
Keyword(s):  
Glucose Uptake ◽  
Carbohydrate Metabolism ◽  
Glycogen Synthesis ◽  
Rat Diaphragm ◽  
Serum Factor ◽  
Inhibitory Factors ◽  
Fasted Rats ◽  
Isolated Rat

A factor has been found in the serum of fasted rats which inhibits glucose uptake and glycogen synthesis in the isolated rat diaphragm. It does not affect CO2 production or O2 uptake. It is nondialyzable, stable in the cold and also stable when heated to 58°C for 1 hour. It was found in Cohn fraction IV-V. Its action did not resemble any of the known hormones, nor could it be identified as one of the inhibitory factors found in diabetic serum.

THE INFLUENCE OF POTASSIUM ION UPON GLUCOSE UPTAKE AND GLYCOGEN SYNTHESIS IN THE ISOLATED RAT DIAPHRAGM

1955 ◽  
Vol 33 (4) ◽  
pp. 687-694 ◽  
Author(s):  
D. W. Clarke
Keyword(s):  
Glucose Uptake ◽  
Glycogen Synthesis ◽  
Potassium Ion ◽  
Ion Concentration ◽  
Rat Diaphragm ◽  
High Potassium ◽  
High Concentrations ◽  
Glycogen Breakdown ◽  
Isolated Rat

The amounts of glucose taken from a medium, and the amounts of glycogen synthesized, by rat hemidiaphragms were studied under various conditions. High concentrations of potassium ion inhibited the glucose uptake and there was also a reduced net glycogen synthesis. Glycogen breakdown was probably not increased by high potassium ion concentration. The effect of potassium was most marked when conditions were such that one would ordinarily expect a considerable glucose uptake or glycogen synthesis. The action of insulin was not peculiarly susceptible to potassium ion inhibition.

scholarly journals THE EFFECT OF GROWTH HORMONE ON GLUCOSE UPTAKE BY THE ISOLATED RAT DIAPHRAGM

1952 ◽  
Vol 197 (1) ◽  
pp. 151-166 ◽  
Author(s):  
C.R. Park ◽  
David H. Brown ◽  
Marvin. Cornblath ◽  
William H. Daughaday ◽  
M.E. Krahl
Keyword(s):  
Growth Hormone ◽  
Glucose Uptake ◽  
Rat Diaphragm ◽  
Isolated Rat

In vitro Effect of Growth Hormone on the Glucose Uptake of Isolated Rat Diaphragm

Nature ◽  
1957 ◽  
Vol 179 (4557) ◽  
pp. 472-473 ◽  
Author(s):  
P. J. RANDLE ◽  
J. E. WHITNEY
Keyword(s):  
Growth Hormone ◽  
Glucose Uptake ◽  
Rat Diaphragm ◽  
Vitro Effect ◽  
Isolated Rat

THE EFFECT OF GROWTH HORMONE AND OF CORTISOL ON THE RESPONSE OF ISOLATED RAT DIAPHRAGM TO THE STIMULATING EFFECT OF INSULIN ON GLUCOSE UPTAKE AND ON INCORPORATION OF AMINO ACIDS INTO PROTEIN

1959 ◽  
Vol 18 (4) ◽  
pp. 395-408 ◽  
Author(s):  
K. L. MANCHESTER ◽  
P. J. RANDLE ◽  
F. G. YOUNG
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Protein Synthesis ◽  
Glucose Uptake ◽  
Carbohydrate Metabolism ◽  
Rat Diaphragm ◽  
Pituitary Growth Hormone ◽  
Isolated Rat ◽  
Stimulation Of

SUMMARY 1. The effect of hypophysectomy, or of adrenalectomy, and injection of pituitary growth hormone (GH) or of cortisol, on the uptake of glucose and the incorporation of glycine into protein by isolated rat diaphragm, and the effect of the addition of insulin in vitro on these processes, has been studied. 2. Both hypophysectomy and adrenalectomy raised the uptake of glucose by isolated diaphragm, while treatment of the intact or of the hypophysectomized rat with GH, or of the intact or of the adrenalectomized rat with cortisol, depressed it. Although hypophysectomy and adrenalectomy did not influence the additional glucose uptake induced by 200 mu./ml. of insulin in vitro, both these operations enhanced the effect of 0·1–1·0 mu./ml. of insulin on glucose uptake by diaphragm in vitro. Treatment of the rat with GH or cortisol diminished the rise in glucose uptake of diaphragm induced by 0·1–1·0 mu./ml. insulin. 3. Hypophysectomy depressed, and administration of GH to the intact or hypophysectomized rat raised, the incorporation of glycine into protein of the isolated diaphragm, but neither of these operations altered the magnitude of the stimulation of incorporation induced by 1·0 mu./ml. insulin. 4. Adrenalectomy raised, and administration of cortisol to the intact or adrenalectomized rat depressed, the incorporation of glycine into protein of the isolated diaphragm; adrenalectomy enhanced, the injection of cortisol diminished, the effect of 1·0 mu./ml. insulin on these processes. 5. The possibility that GH directs insulin towards the stimulation of protein synthesis, in part by restraining the action of insulin on carbohydrate metabolism, is discussed.

Glucose Uptake and Response to Insulin of the Isolated Rat Diaphragm: The Effect of Denervation

Diabetes ◽  
1959 ◽  
Vol 8 (3) ◽  
pp. 218-225 ◽  
Author(s):  
M. G. Buse ◽  
J. Buse ◽  
E. Porter
Keyword(s):  
Glucose Uptake ◽  
Rat Diaphragm ◽  
Isolated Rat

scholarly journals RELATIVE POTENCY OF ANTI-DIABETIC COSTUS SPECIOSUSPLANT EXTRACT IN MOUSE FIBROBLAST CELL LINE

2016 ◽  
Vol 4 (3) ◽  
pp. 99-107
Author(s):  
Bindu ◽  
Rama Bhat ◽  
Girish ◽  
Krishna Prasad
Keyword(s):  
Glucose Uptake ◽  
Biological Activities ◽  
Glycogen Synthesis ◽  
In Vitro Study ◽  
Significant Inhibition ◽  
Mouse Fibroblast ◽  
Relative Potency ◽  
Major Pathway ◽  
Chemical Structures

Plant-derived compounds have been used clinically to treat type 2 diabetes for many years as they also exert additional beneficial effects on various other disorders. PI3K pathway is the major pathway activated by insulin receptor (IR). It induces glucose uptake, glycogen synthesis, protein synthesis, cell growth and differentiation. Hence metabolic assay was employed to assess glucose uptake based on the property of 3T3-L 1 cells to differentiate into adipocytes which can take up the glucose in medium due to the effect of insulin or insulin like molecules. The results of the current study showed that plants extract probably exerts its anti-diabetic properties by stimulating glucose uptake in adipocytes with significant inhibition of adipogenesis demonstrating reliable relative potency in comparision to the commercial insulin.  The ability of existing therapies to target various aspects of the insulin resistance syndrome induces other metabolic abnormalities, chiefly those involved in lipid metabolism. In this preliminary in-vitro study Costusspeciosus plant extract demonstrated to have significant relative potency in comparison to commercial Insulin which can be exploited to treat diabetes using natural herbal extracts Current study leads researchers to elucidate the chemical structures, isolate active ingrediatents in the crude extract for such biological activities in reference to commercial and recombinant insulins.

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