scholarly journals Quantitative determination of urea concentrations in cell culture medium

2009 ◽  
Vol 87 (3) ◽  
pp. 541-544 ◽  
Author(s):  
Robert J.X. Zawada ◽  
Peggy Kwan ◽  
Kellen L. Olszewski ◽  
Manuel Llinas ◽  
Shu-Gui Huang
Keyword(s):  
Cell Culture ◽  
Quantitative Determination ◽  
Protein Metabolism ◽  
Room Temperature ◽  
Culture Medium ◽  
Urea Synthesis ◽  
Cell Culture Medium ◽  
Urea Determination ◽  
Sensitive Method

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.

Quantitative determination of VEGF165 in cell culture medium by aptamer sandwich based chemiluminescence assay

Talanta ◽  
2017 ◽  
Vol 171 ◽  
pp. 197-203 ◽  
Author(s):  
Siwen Shan ◽  
Ziyi He ◽  
Sifeng Mao ◽  
Mingsha Jie ◽  
Linglu Yi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Quantitative Determination ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Chemiluminescence Assay

HPLC determination of malondialdehyde in ECV304 cell culture medium for measuring the antioxidant effect of vitexin-4″-O-glucoside

2008 ◽  
Vol 31 (7) ◽  
pp. 878-885 ◽  
Author(s):  
Xi-xiang Ying ◽  
Hai-bo Li ◽  
Zheng-yun Chu ◽  
Yan-jun Zhai ◽  
Ai-jing Leng ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Antioxidant Effect ◽  
Hplc Determination ◽  
Cell Culture Medium ◽  
Ecv304 Cell
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