Determination of cell culture medium components with overlapping near-IR absorbance peaks

1998 ◽  
Author(s):  
Michael J. McShane ◽  
Gerard L. Cote
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Medium Components ◽  
Near Ir

Effect of cell culture medium components on color of formulated monoclonal antibody drug substance

2013 ◽  
Vol 29 (5) ◽  
pp. 1270-1277 ◽  
Author(s):  
Natarajan Vijayasankaran ◽  
Sharat Varma ◽  
Yi Yang ◽  
Melissa Mun ◽  
Silvana Arevalo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Medium ◽  
Drug Substance ◽  
Cell Culture Medium ◽  
Medium Components ◽  
Antibody Drug

scholarly journals Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?

2017 ◽  
Vol 6 (1) ◽  
pp. 1272832 ◽  
Author(s):  
Juan Pablo Tosar ◽  
Alfonso Cayota ◽  
Erez Eitan ◽  
Marc K. Halushka ◽  
Kenneth W. Witwer
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Extracellular Rna ◽  
Medium Components

scholarly journals Quantitative determination of urea concentrations in cell culture medium

2009 ◽  
Vol 87 (3) ◽  
pp. 541-544 ◽  
Author(s):  
Robert J.X. Zawada ◽  
Peggy Kwan ◽  
Kellen L. Olszewski ◽  
Manuel Llinas ◽  
Shu-Gui Huang
Keyword(s):  
Cell Culture ◽  
Quantitative Determination ◽  
Protein Metabolism ◽  
Room Temperature ◽  
Culture Medium ◽  
Urea Synthesis ◽  
Cell Culture Medium ◽  
Urea Determination ◽  
Sensitive Method

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.

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