An antibody-conjugated internalizing quantum dot suitable for long-term live imaging of cells

2007 ◽  
Vol 85 (1) ◽  
pp. 133-140 ◽  
Author(s):  
Zeenia Kaul ◽  
Tomoko Yaguchi ◽  
Jun I. Harada ◽  
Yutaka Ikeda ◽  
Takashi Hirano ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Molecular Imaging ◽  
Fluorescent Dyes ◽  
Organic Dyes ◽  
Semiconductor Nanocrystals ◽  
Biological Applications ◽  
Therapeutic Tool ◽  
Sensitive Tool ◽  
Antibody Conjugate

Quantum dots (QD) are fluorescent semiconductor nanocrystals that are emerging as superior alternatives to the conventional organic dyes used in biological applications. Although QDs offer several advantages over conventional fluorescent dyes, including greater photostability and a wider range of excitation and (or) emission wavelengths, their toxicity has been an issue in its wider use as an analytic, diagnostic and therapeutic tool. We prepared a conjugate QD with an internalizing antibody and demonstrated that the QD–antibody conjugate is efficiently internalized into cells and is visible even after multiple divisions. We demonstrate that the internalized QD is nontoxic to cells and provides a sensitive tool for long-term molecular imaging.

Application of quantum dots in drug delivery

2021 ◽  
Vol 11 ◽  
Author(s):  
Subham Jain N ◽  
Preeti S ◽  
Amit B Patil
Keyword(s):  
Drug Delivery ◽  
Quantum Dots ◽  
Organic Dyes ◽  
Quantum Confinement Effect ◽  
Semiconductor Nanocrystals ◽  
Drug Carriers ◽  
Research Field ◽  
The Body ◽  
Future Application ◽  
Potential Candidate

Background: The nanotechnology which has vast growth in the research field and the outcome product of nanotechnology is nanoparticles. Quantum dots with a size range of 2-10nm represents a new form in nanotechnology materials. It has showed widespread attention in recent years in the field of science and its application in drug delivery. Quantum dots are semiconductor nanocrystals which possess interesting properties and characteristics such as unique optical properties, quantum confinement effect and emit fluorescence on excitation with a light source which makes them a potential candidate for nano-probes and for carriers for biological application. Objective: The objective of the article is to explain the role and application of Quantum dots in drug delivery and its future application in pharmaceutical science and research. This review focuses on drug delivery through Quantum dots and Quantum dots helping nanocarriers for drug delivery. The development of QD nano-carriers for drugs has become a hotspot in the fields of nano-drug research. The Quantum Dot labelled nano-carrier can able to deliver the drugs with fewer side effects and it can able to trace the drug location in the body. Results: The Fluorescent emission of Quantum dots is better than other organic dyes which leads to better drug delivery for cancer or acting as a tag for other drug carriers. Conclusion: Because of emission property of Quantum Dots, it can be said used with other drug carriers and later it can be traced with the help of Quantum Dots. Quantum dots can be said as smart Drug delivery.

scholarly journals Loading Graphene Quantum Dots into Optical-Magneto Nanoparticles for Real-Time Tracking In Vivo

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2191 ◽  
Author(s):  
Yu Wang ◽  
Nan Xu ◽  
Yongkai He ◽  
Jingyun Wang ◽  
Dan Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Real Time ◽  
Fluorescent Dyes ◽  
Graphene Quantum Dots ◽  
Shell Nanoparticles ◽  
One Pot ◽  
Real Time Tracking

Fluorescence imaging offers a new approach to visualize real-time details on a cellular level in vitro and in vivo without radioactive damage. Poor light stability of organic fluorescent dyes makes long-term imaging difficult. Due to their outstanding optical properties and unique structural features, graphene quantum dots (GQDs) are promising in the field of imaging for real-time tracking in vivo. At present, GQDs are mainly loaded on the surface of nanoparticles. In this study, we developed an efficient and convenient one-pot method to load GQDs into nanoparticles, leading to longer metabolic processes in blood and increased delivery of GQDs to tumors. Optical-magneto ferroferric oxide@polypyrrole (Fe3O4@PPy) core-shell nanoparticles were chosen for their potential use in cancer therapy. The in vivo results demonstrated that by loading GQDs, it was possible to monitor the distribution and metabolism of nanoparticles. This study provided new insights into the application of GQDs in long-term in vivo real-time tracking.

The Cytotoxicity of Quantum Dots CdSe/CdS functionalized with -COOH and –NH2

2009 ◽  
Vol 1220 ◽  
Author(s):  
Lin-Jing Shen ◽  
Jing Cui ◽  
Jin-Hua Liu ◽  
Xiao-Bo Xu ◽  
Ming-Qiang Zhu
Keyword(s):  
Quantum Dots ◽  
Fluorescent Dyes ◽  
Semiconductor Nanocrystals ◽  
Assay Method ◽  
Dependent Effect ◽  
Lower Toxicity ◽  
Size Dependent ◽  
Dose Dependent ◽  
Dose Dependent Effect ◽  
Mtt Assays

AbstractRecently, semiconductor nanocrystals or quantum dots (QDs) aroused great concern because of their unique properties such as the size-dependent photoluminescence. They have many excellent applications in areas of molecular bioimaging, medical detection and even energy, especially as biosensing and imaging instead of fluorescent dyes. For the bio-safety, however, we should assess the cytotoxicity of QDs before used in biomedical imaging. Here, the cytotoxicity of amino-functionalized CdSe/CdS (CdSe/CdS-NH2) QDs and carboxy-functionalized CdSe/CdS (CdSe/CdS-COOH) QDs was investigated by MTT assay method. According to our findings, both CdSe/CdS-NH2 and CdSe/CdS-COOH have a dose-dependent effect on cell proliferation. The cytotoxicity of QDs varies with storing time of QDs and kinds of cells. The cytotoxicity of QDs modified with -COOH or -NH2 groups both vary with concentrations in positive linear or change with QD storing time in negative linear. The results indicate that CdSe/CdS-COOH QDs have lower toxicity than CdSe/CdS-NH2 QDs. Hela cell is somewhat more sensitive to amino- and carboxy-modified QDs than Bel7404 cell for MTT assays.

scholarly journals Quantum dot probes for cellular analysis

2017 ◽  
Vol 9 (18) ◽  
pp. 2621-2632 ◽  
Author(s):  
Dahai Ren ◽  
Bin Wang ◽  
Chen Hu ◽  
Zheng You
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Semiconductor Nanocrystals ◽  
Pivotal Role ◽  
Biological Applications ◽  
Cellular Analysis

Highly fluorescent and robust semiconductor nanocrystals (known as quantum dots or QDs) play a pivotal role in biological applications.

scholarly journals Application of Quantum Dots in Biological Imaging

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Shan Jin ◽  
Yanxi Hu ◽  
Zhanjun Gu ◽  
Lei Liu ◽  
Hai-Chen Wu
Keyword(s):  
Quantum Dots ◽  
Organic Dyes ◽  
Rapid Development ◽  
Emission Spectra ◽  
Major Type ◽  
Biological Applications ◽  
Biological Studies ◽  
Polymer Dots

Quantum dots (QDs) are a group of semiconducting nanomaterials with unique optical and electronic properties. They have distinct advantages over traditional fluorescent organic dyes in chemical and biological studies in terms of tunable emission spectra, signal brightness, photostability, and so forth. Currently, the major type of QDs is the heavy metal-containing II-IV, IV-VI, or III-V QDs. Silicon QDs and conjugated polymer dots have also been developed in order to lower the potential toxicity of the fluorescent probes for biological applications. Aqueous solubility is the common problem for all types of QDs when they are employed in the biological researches, such asin vitroandin vivoimaging. To circumvent this problem, ligand exchange and polymer coating are proven to be effective, besides synthesizing QDs in aqueous solutions directly. However, toxicity is another big concern especially forin vivostudies. Ligand protection and core/shell structure can partly solve this problem. With the rapid development of QDs research, new elements and new morphologies have been introduced to this area to fabricate more safe and efficient QDs for biological applications.

Quantum Dots Based Optical Sensors

2012 ◽  
Vol 326-328 ◽  
pp. 682-689
Author(s):  
Aleksandra Lobnik ◽  
Špela Korent Urek ◽  
Matejka Turel
Keyword(s):  
Quantum Dots ◽  
Optical Sensors ◽  
Organic Dyes ◽  
Semiconductor Nanocrystals ◽  
Short Review ◽  
Chemical Systems ◽  
Luminescent Sensors ◽  
Luminescent Nanoparticles ◽  
Points Of View ◽  
New Applications

Luminescent sensors are chemical systems that can deliver information on the presence of selected analytes through the variations in their luminescence emission. With the advent of luminescent nanoparticles several new applications in the field of chemical sensing were explored. Among them, quantum dots (QD) represent inorganic semiconductor nanocrystals that are advantageous over conventional organic dyes from many different points of view. In this short review, the optical detection of various analytes using QD-based probes/sensors is presented and significant sensors characteristics are discussed. The biosensing approaches are not included in this article.

scholarly journals Synthesis, Characterization and Evaluation of the Cytotoxicity of Ni-Doped Zn(Se,S) Quantum Dots

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Melissa Cruz-Acuña ◽  
Sonia Bailón-Ruiz ◽  
Carlos R. Marti-Figueroa ◽  
Ricardo Cruz-Acuña ◽  
Oscar J. Perales-Pérez
Keyword(s):  
Drug Delivery ◽  
Quantum Dots ◽  
Optical Properties ◽  
Cell Viability ◽  
Zinc Chloride ◽  
Semiconductor Nanocrystals ◽  
Carcinoma Cells ◽  
Biological Applications ◽  
Potential Toxicity ◽  
Light Excitation

Quantum dots (QDs) are semiconductor nanocrystals with desirable optical properties for biological applications, such as bioimaging and drug delivery. However, the potential toxicity of these nanostructures in biological systems limits their application. The present work is focused on the synthesis, characterization, and evaluation of the toxicity of water-stable Ni-doped Zn(Se,S) QDs. Also, the study of nondoped nanostructures was included for comparison purposes. Ni-doped nanostructures were produced from zinc chloride and selenide aqueous solutions in presence of 3-mercaptopropionic acid and Ni molar concentration of 0.001 M. In order to evaluate the potential cytoxicity of these doped nanostructures, human pancreatic carcinoma cells (PANC-1) were used as model. The cell viability was monitored in presence of Ni-doped Zn(Se,S) QDs at concentrations ranging from 0 μg/mL to 500 μg/mL and light excited Ni-doped Zn(Se,S) nanostructures were evaluated at 50 μg/mL. Results suggested that Ni-doped Zn(Se,S) nanostructures were completely safe to PANC-1 when concentrations from 0 μg/mL to 500 μg/mL were used, whereas non-doped nanostructures evidenced toxicity at concentrations higher than 200 μg/mL. Also, Ni-doped Zn(Se,S) QDs under light excitation do not evidence toxicity to PANC-1. These findings suggest strongly that Zn(Se,S) nanostructures doped with nickel could be used in a safe manner in light-driving biological applications and drug delivery.

scholarly journals Multimodal optical studies of single and clustered colloidal quantum dots for the long-term optical property evaluation of quantum dot-based molecular imaging phantoms

2012 ◽  
Vol 3 (6) ◽  
pp. 1312 ◽  
Author(s):  
HyeongGon Kang ◽  
Matthew L. Clarke ◽  
Silvia H. De Paoli Lacerda ◽  
Alamgir Karim ◽  
Leonard F. Pease ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Molecular Imaging ◽  
Optical Property ◽  
Quantum Dot ◽  
Colloidal Quantum Dots ◽  
Optical Studies ◽  
Property Evaluation ◽  
Imaging Phantoms

Preparation of Graphene Quantum Dots and Their Biological Applications

2016 ◽  
Vol 31 (4) ◽  
pp. 337 ◽  
Author(s):  
SUN Xiao-Dan ◽  
LIU Zhong-Qun ◽  
YAN Hao
Keyword(s):  
Quantum Dots ◽  
Graphene Quantum Dots ◽  
Biological Applications

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

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