In vitro colonization and increase in nitrogen fixation of seedling roots of black mangrove inoculated by a filamentous cyanobacteria

1995 ◽  
Vol 41 (11) ◽  
pp. 1012-1020 ◽  
Author(s):  
Gerardo Toledo ◽  
Yoav Bashan ◽  
Al Soeldner
Keyword(s):  
Nitrogen Fixation ◽  
Root Colonization ◽  
Avicennia Germinans ◽  
Plant Growth Promoting Bacteria ◽  
Filamentous Cyanobacterium ◽  
Seedling Roots ◽  
Plant Growth Promoting ◽  
Black Mangrove ◽  
Similar Quantity

An isolate of the filamentous cyanobacterium Microcoleus sp. was obtained from black mangrove aerial root (pneumatophore) and inoculated onto young mangrove seedlings to evaluate N2-fixation and root-colonization capacities of the bacterium under in vitro conditions in closed-system experiments. N2 fixation (acetylene reduction) gradually increased with time and reached its peak 5 days after inoculation. Later, it decreased sharply. The level of N2 fixation in the presence of the plant was significantly higher than the amount of nitrogen fixed by a similar quantity of cyanobacteria on a N-free growth medium. The main feature of this root colonization was the gradual production of a biofilm in which the cyanobacterial filaments were embedded. Visible biofilm production increased with time until it completely covered the entire root system of the plant. The in-and-out movement of cyanobacterial filaments from the biofilm probably allows colonization of uncolonized portions of the root several days after the initial inoculation. This is, to the best of our knowledge, the first report of the artificial inoculation of cyanobacteria on marine mangroves.Key words: Avicennia germinans, beneficial bacteria, biofilm, black mangrove, diazotrophic cyanobacteria, Microcoleus sp., nitrogen fixation, plant growth-promoting bacteria, root colonization.

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

2015 ◽  
Vol 3 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Mohamed A.M. El-Awady ◽  
Mohamed M. Hassan ◽  
Yassin M. Al-Sodany
Keyword(s):  
Nitrogen Fixation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Promotion ◽  
Exponential Growth Phase ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Growth Promoting

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

scholarly journals COLONIZATION of Rubus glaucus BENTH BY AZORHIZOBIUM CAULINODANS ORS571 USIG A FLAVONOIDE NARINGENINA

2016 ◽  
Vol 14 (2) ◽  
pp. 15
Author(s):  
Giovani Orlando Cancino Escalante ◽  
S E Cancino ◽  
Enrique Quevedo Garcia
Keyword(s):  
Bacterial Colonization ◽  
Lateral Roots ◽  
Plant Growth Promoting Bacteria ◽  
Azorhizobium Caulinodans ◽  
Intensity Parameters ◽  
Plant Growth Promoting ◽  
Measuring Frequency ◽  
Northwest Region ◽  
Azorhizobium Caulinodans Ors571

Root systems of two Andean blackberry materials (thorn and thornless) of Rubus glaucus Benth cultured in vitro in the presence of five treatments (four flavonoids and one control) were inoculated with Azorhizobium caulinodans ORS571 (pXLGD4)  (a strain carrying the lacZ reporter gene which facilitated the detection of bacterial colonization). Evaluation of colonization effectiveness for each treatment was done by means of application of experimental design measuring frequency and intensity parameters. Statistical analysis showed differences at comparing flavonoids vs. control and the overall higher effectiveness of the flavonoid naringenin. Observation of colonization was made by light and electron microscope confirming internal colonization of Andean blackberry roots by A. caulinodans. This is the first work demonstrating root colonization of R.glaucus by azorhizobia and therefore settling the basis for future investigations and scientific applications related to interaction with plant growth-promoting bacteria under the effect of flavonoids, along with possible implications of common benefit for non-legume crops in the northwest region of Colombia.  Key Words: Azorhizobium caulinodans ORS571, Andean blackberry, flavonoids, LacZ, lateral roots, naringenin. 

scholarly journals A Survey of Methylobacterium Species and Strains Reveals Widespread Production and Varying Profiles of Cytokinin Phytohormones

2021 ◽  
Author(s):  
Daniel Palberg ◽  
Anna Kisiała ◽  
Gabriel Lemes Jorge ◽  
R. J. Neil Emery
Keyword(s):  
High Performance ◽  
Active Form ◽  
Plant Growth Promoting Bacteria ◽  
Liquid Chromatography Tandem Mass ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Selection For ◽  
Indole 3 Acetic Acid

Abstract BackgroundSymbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), including the most active form, trans-Zeatin (tZ). ResultsThis study provides a comprehensive inventory of 46 representatives of Methylobacterium genus with respect to phytohormone production in vitro, including 16 CK forms, abscisic acid (ABA) and indole-3-acetic acid (IAA). High performance-liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) analyses revealed varying abilities of Methylobacterium strains to secrete phytohormones that ranged from 5.09 to 191.47 pmol mL-1 for total CKs, and 0.46 to 82.16 pmol mL-1 for tZ. Results indicate that reduced methanol availability, the sole carbon source for bacteria in the medium, stimulates CK secretion by Methylobacterium. Additionally, select strains were able to transform L-tryptophan into IAA while no ABA production was detected.ConclusionsTo better understand features of CKs in plants, this study uncovers CK profiles of Methylobacterium that are instrumental in microbe selection for effective biofertilizer formulations.

scholarly journals Compatibility of Azospirillum brasilense with Pesticides Used for Treatment of Maize Seeds

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mariana S. Santos ◽  
Artur B. L. Rondina ◽  
Marco A. Nogueira ◽  
Mariangela Hungria
Keyword(s):  
Plant Growth ◽  
Root Hair ◽  
Azospirillum Brasilense ◽  
Plant Protection ◽  
Plant Growth Promoting Bacteria ◽  
Root Volume ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Seed treatment with chemical pesticides is commonly used as an initial plant protection procedure against pests and diseases. However, the use of such chemicals may impair the survival and performance of beneficial microorganisms introduced via inoculants, such as the plant growth-promoting bacterium Azospirillum brasilense. We assessed the compatibility between the most common pesticide used in Brazil for the treatment of maize seeds, composed of two fungicides, and one insecticide, with the commercial strains Ab-V5 and Ab-V6 of A. brasilense, and evaluated the impacts on initial plant development. The toxicity of the pesticide to A. brasilense was confirmed, with an increase in cell mortality after only 24 hours of exposure in vitro. Seed germination and seedling growth were not affected neither by the A. brasilense nor by the pesticide. However, under greenhouse conditions, the pesticide affected root volume and dry weight and root-hair incidence, but the toxicity was alleviated by the inoculation with A. brasilense for the root volume and root-hair incidence parameters. In maize seeds inoculated with A. brasilense, the pesticide negatively affected the number of branches, root-hair incidence, and root-hair length. Therefore, new inoculant formulations with cell protectors and the development of compatible pesticides should be searched to guarantee the benefits of inoculation with plant growth-promoting bacteria.

Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings

2001 ◽  
Vol 47 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Donna M Penrose ◽  
Barbara A Moffatt ◽  
Bernard R Glick
Keyword(s):  
Carboxylic Acid ◽  
Plant Growth ◽  
Root Elongation ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Psychrophilic Bacterium ◽  
Ethylene Inhibitor ◽  
Seedling Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ*Tag Amino Acid Analysis Method(tm) was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

scholarly journals Growth promotion of pineapple 'vitória' by humic acids and burkholderia spp. during acclimatization

2010 ◽  
Vol 34 (5) ◽  
pp. 1593-1600 ◽  
Author(s):  
Lílian Estrela Borges Baldotto ◽  
Marihus Altoé Baldotto ◽  
Luciano Pasqualoto Canellas ◽  
Ricardo Bressan-Smith ◽  
Fábio Lopes Olivares
Keyword(s):  
Humic Acids ◽  
Growth Promotion ◽  
Combined Treatment ◽  
Plant Growth Promoting Bacteria ◽  
Ex Vitro ◽  
Nutrient Contents ◽  
Planting Material ◽  
Plant Growth Promoting ◽  
The Stability

In vitro propagation of pineapple produces uniform and disease-free plantlets, but requires a long period of acclimatization before transplanting to the field. Quicker adaptation to the ex vitro environment and growth acceleration of pineapple plantlets are prerequisites for the production of a greater amount of vigorous, well-rooted planting material. The combination of humic acids and endophytic bacteria could be a useful technological approach to reduce the critical period of acclimatization. The aim of this study was to evaluate the initial performance of tissue-cultured pineapple variety Vitória in response to application of humic acids isolated from vermicompost and plant growth-promoting bacteria (Burkholderia spp.) during greenhouse acclimatization. The basal leaf axils were treated with humic acids while roots were immersed in bacterial medium. Humic acids and bacteria application improved shoot growth (14 and 102 %, respectively), compared with the control; the effect of the combined treatment was most pronounced (147 %). Likewise, humic acids increased root growth by 50 %, bacteria by 81 % and the combined treatment by 105 %. Inoculation was found to significantly increase the accumulation of N (115 %), P (112 %) and K (69 %) in pineapple leaves. Pineapple growth was influenced by inoculation with Burkholderia spp., and further improved in combination with humic acids, resulting in higher shoot and root biomass as well as nutrient contents (N 132 %, P 131 %, K 80 %) than in uninoculated plantlets. The stability and increased consistency of the host plant response to bacterization in the presence of humic substances indicate a promising biotechnological tool to improve growth and adaptation of pineapple plantlets to the ex vitro environment.

scholarly journals Acclimatization of Pouteria gardeneriana Radlk micropropagated plantlets: role of in vitro rooting and plant growth–promoting bacteria

2021 ◽  
pp. 100209
Author(s):  
Mariluza Silva Leite ◽  
Tainara Eler Furtado Pinto ◽  
Agda Rabelo Centofante ◽  
Aurélio Rubio Neto ◽  
Fabiano Guimarães Silva ◽  
...  
Keyword(s):  
Plant Growth ◽  
In Vitro Rooting ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens

2021 ◽  
Vol 9 (4) ◽  
pp. 682
Author(s):  
Mohamad Syazwan Ngalimat ◽  
Erneeza Mohd Hata ◽  
Dzarifah Zulperi ◽  
Siti Izera Ismail ◽  
Mohd Razi Ismail ◽  
...  
Keyword(s):  
Plant Growth ◽  
Large Scale ◽  
Plant Growth Promoting Bacteria ◽  
Promising Alternative ◽  
Rice Plants ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rice Pathogens ◽  
Grain Losses

As a major food crop, rice (Oryza sativa) is produced and consumed by nearly 90% of the population in Asia with less than 9% produced outside Asia. Hence, reports on large scale grain losses were alarming and resulted in a heightened awareness on the importance of rice plants’ health and increased interest against phytopathogens in rice. To serve this interest, this review will provide a summary on bacterial rice pathogens, which can potentially be controlled by plant growth-promoting bacteria (PGPB). Additionally, this review highlights PGPB-mediated functional traits, including biocontrol of bacterial rice pathogens and enhancement of rice plant’s growth. Currently, a plethora of recent studies address the use of PGPB to combat bacterial rice pathogens in an attempt to replace existing methods of chemical fertilizers and pesticides that often lead to environmental pollutions. As a tool to combat bacterial rice pathogens, PGPB presented itself as a promising alternative in improving rice plants’ health and simultaneously controlling bacterial rice pathogens in vitro and in the field/greenhouse studies. PGPB, such as Bacillus, Pseudomonas, Enterobacter, Streptomyces, are now very well-known. Applications of PGPB as bioformulations are found to be effective in improving rice productivity and provide an eco-friendly alternative to agroecosystems.

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