Development of a practical method for inducing white-rot fungi to grow into and degrade organopollutants in soil

1995 ◽  
Vol 41 (4-5) ◽  
pp. 345-353 ◽  
Author(s):  
C. David Boyle
Keyword(s):  
White Rot Fungi ◽  
High Growth ◽  
Nitrogen Sources ◽  
Practical Method ◽  
White Rot ◽  
Plant Residues ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Hazardous Organic Compounds ◽  
Land Farming

White-rot fungi degrade many hazardous organic compounds that are not readily degraded by other microorganisms. Some of these compounds are soil contaminants, so methods for using these fungi to decontaminate soil through either land farming or composting technologies are being developed. White-rot fungi normally colonize plants or plant residues (e.g., wood) and do not grow well in unamended soil, particularly if it is not sterilized. A practical method to promote their growth in soil, without the use of large quantities of amendments or inoculum, is presented. A variety of assays showed that growth of white-rot fungi in steamed soil is limited by availability of carbon and nitrogen sources, but not other nutrients. Ground alfalfa straw was a more effective inexpensive source of these nutrients than the other amendments that were tested. However, the fungi only sometimes colonized alfalfa-amended nonsterile soil, as a result of competition from other microorganisms. Consistently high growth of the white-rot fungi in alfalfa-amended soil could be induced by adjusting the moisture content, adding the fungicide benomyl, and inoculating with benomyl-resistant fungi. In soil so treated, degradation (mineralization) of pentachlorophenol was much more rapid than in untreated soil.Key words: white-rot fungi, bioremediation, growth, pentachlorophenol.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Role of Enzymatic System of Screened Pleurots ostreatus IBL-02 in the Bio-Removal of Synthetic Dyes Effluent

2019 ◽  
Vol 41 (3) ◽  
pp. 509-509
Author(s):  
Tahsin Gulzar Tahsin Gulzar ◽  
Shumaila Kiran Shumaila Kiran ◽  
Shazia Abrar Shazia Abrar ◽  
Muniba Rahmat Muniba Rahmat ◽  
Asma Haque Asma Haque ◽  
...  
Keyword(s):  
Spectral Line ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Nitrogen Sources ◽  
White Rot ◽  
Synthetic Dyes ◽  
Enzymatic System ◽  
Carbon And Nitrogen ◽  
Physico Chemical

White rot fungi have exclusive capacity to decolorize synthetic azo dyes. Five different fungal strains namely S. commune IBL-01 (SC), P. ostreatus IBL-02 (PO) P. chrysosporium IBL-03 (PC), T. versicolor IBL-04 (TV) and G. lucidum IBL-05 (GL) were used for the decolorization of synthetic dyes effluent. P. ostreatus IBL-02 showed maximum decolorization of synthetic textile effluent, so it was selected for the optimization of experimental factors. Different physico-chemical parameters were optimized using Pleutorus ostreatus IBL-02 (PO), for the maximum de-colorization of synthetic dyes effluent. Under optimum conditions, P. ostreatusIBL-02 (PO) decolorized the synthetic dyes effluent by 92.7%. Effect of various amendments like carbon and nitrogen sources on the decolorization and mineralization of synthetic dyes effluent by Pleutorus ostreatus IBL-02 was also studied. Ligninolytic enzymes at the end of each experiment were studied to find their role in decolorization and mineralization of synthetic dyes effluent. UV-Visible spectral analysis was used to indicate the decolourization of treated dyes effluent sample. A diminution in spectral line height displaying absorption maxima designated decolorization (%) while a move of spectral line to the UV-region depicted the degradation of synthetic dyes effluent.

Physiological control of trophophase–idiophase separation in streptomycete cultures producing secondary metabolites

1995 ◽  
Vol 41 (4-5) ◽  
pp. 309-315 ◽  
Author(s):  
Xiaowen Liao ◽  
Leo C. Vining ◽  
Janice L. Doull
Keyword(s):  
Secondary Metabolites ◽  
Streptomyces Coelicolor ◽  
High Growth ◽  
Biomass Accumulation ◽  
Nitrogen Sources ◽  
Streptomyces Venezuelae ◽  
Physiological Control ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Defined Media

Cultures of Streptomyces coelicolor A3 (2) produced actinorhodin in defined media with various carbon and nitrogen sources. Production occurred during biomass accumulation if assimilation of either the carbon or the nitrogen source limited the rate of growth. High growth rates tended to delay product synthesis until after biomass accumulation was complete, but fully biphasic fermentation profiles were achieved only with media supporting very rapid growth. The onset of actinorhodin production then coincided with a decline in the growth rate during transition of carbon-sufficient cultures to stationary phase. In cultures with maltose as a growth-limiting carbon source, depletion of phosphate increased the rate of actinorhodin biosynthesis, but did not alter the timing of its initiation. With defined media, the use of spores rather than vegetative mycelium as inocula reduced the overlap between trophophase and idiophase. The general guidelines for achieving biphasic production of actinorhodin in S. coelicolor A3 (2) cultures could be used to obtain trophophase–idiophase separation in cultures of Streptomyces venezuelae producing chloramphenicol. However, the conditions needed to be modified to give optimized biphasic fermentations with individual strains. Under conditions favouring chloramphenicol production in a distinct idiophase, aromatic amine secondary metabolites in the same cultures of S. venezuelae were produced in a pattern that overlapped the trophophase, suggesting that conditions need to be tailored also to meet differences in the regulation of secondary metabolites.Key words: Streptomyces coelicolor A3 (2), Streptomyces venezuelae, actinorhodin, biphasic fermentations, chloramphenicol, inoculum shift down.

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

scholarly journals Optimising carbon and nitrogen sources for Azotobacter chroococcum growth

2011 ◽  
Vol 10 (15) ◽  
pp. 2951-2958 ◽  
Author(s):  
Gutieacute rrez Rojas Ivonne ◽  
Beatriz Torres Geraldo Ana ◽  
Moreno Sarmiento Nubia
Keyword(s):  
Nitrogen Sources ◽  
Azotobacter Chroococcum ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen
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