Study of lipids in Lipomyces and Waltomyces

1994 ◽  
Vol 40 (9) ◽  
pp. 724-729 ◽  
Author(s):  
A. Lomascolo ◽  
E. Dubreucq ◽  
V. Perrier ◽  
P. Galzy
Keyword(s):  
Fatty Acids ◽  
Lipid Composition ◽  
Unsaturated Fatty Acids ◽  
Lipid Fraction ◽  
Short Chain Fatty Acids ◽  
Membrane Phospholipids ◽  
Culture Temperature ◽  
Third Way ◽  
Total Fatty Acids ◽  
Arachidonic Acids

The lipid composition, particularly the fatty acid composition (6–25 carbon atoms), of one strain of each species of Lipomyces and Waltomyces was determined; the influence of the culture temperature on the lipid composition was also studied. The neutral lipid fraction, essentially composed of intracellular triacylglycerols, and the polar fraction, composed of membrane phospholipids, were analyzed separately. For all strains and in the two fractions, the major fatty acids were oleic, linoleic, palmitic, palmitoleic, stearic, and α-linolenic acids, which formed 85% of the total fatty acids. Small amounts of γ-linolenic, dihomo-γ-linolenic, and arachidonic acids were present, which indicated the presence of Δ12, Δ15, Δ6, and Δ5 desaturases. Waltomyces lipofer and Lipomyces tetrasporus were the two species richest in lipids and fatty acids. In the genus Lipomyces and in Waltomyces, maintenance of membrane fluidity at low temperature required an increase in unsaturated fatty acids and the accumulation of short-chain fatty acids, especially in the polar lipid fraction; in the case of Lipomyces anomalus, the synthesis of branched fatty acids may be a third way to maintain membrane fluidity.Key words: Lipomyces, Waltomyces, lipid composition, fatty acids.

scholarly journals The Changes in the Lipid Composition of Mung Bean Seeds as Affected by Processing Methods

2007 ◽  
Vol 3 (5) ◽  
Author(s):  
El-Sayed Ali Abdel-Rahman ◽  
Fawzy A El-Fishawy ◽  
Mohamed A El-Geddawy ◽  
Tomas Kurz ◽  
Mohamed N El-Rify
Keyword(s):  
Fatty Acids ◽  
Lipid Composition ◽  
Mung Bean ◽  
Unsaturated Fatty Acids ◽  
Essential Fatty Acids ◽  
Saturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Lipid Fractions ◽  
Total Fatty Acids ◽  
Tlc Analysis

This study was conducted to assess in detail the possible effects of some technological processes such as soaking, germination, cooking, soaking + cooking, and germination + cooking on the lipid composition of mung bean seeds of Giza 1 variety. TLC analysis of mung bean lipids showed that the phospholipids and triglycerides recorded the highest percentage among lipid fractions (32.26 and 30.10%), while the 1,3 diglycerides constituted the least percentage (2.80%) in mung bean seeds. The soaking, germination and cooking processes caused a decrease in the phospholipids, triglycerides and hydrocarbons accompanied with an increase in monoglycerides, 1,2-(2,3)-diglycerides, sterols and free fatty acids. Eleven fractions were separated from phospholipids class of the studied samples; seven of these fractions were identified. The major component of phospholipids was phosphatidyl choline, amounting to 21.30, 17.84, 16.21, 13.87, 13.20 and 11.47% of the total phospholipids in raw, soaked, germinated, raw-cooked, soaked-cooked and germinated-cooked mung bean seeds, respectively. Gas liquid chromatography of the total lipids of mung bean seeds showed that the unsaturated fatty acids represented 69.58, 64.35, 63.3, 63.16, 61.84 and 61.12%, while the levels of saturated fatty acids were low being 30.37, 34.05, 35.66, 34.64, 37.93 and 38.75% of the total fatty acids in raw, soaked, germinated, raw-cooked, soaked-cooked and germinated-cooked, respectively. The total essential fatty acids (linoleic and linolenic) represented the highest proportion of fatty acids (50.10% of the total fatty acids).

Changes in lipid composition of apple surface layer during long-term storage in controlled atmosphere

2012 ◽  
Vol 66 (10) ◽  
Author(s):  
Kateřina Duroňová ◽  
Ivana Márová ◽  
Milan Čertík ◽  
Stanislav Obruča
Keyword(s):  
Fatty Acids ◽  
Oxygen Content ◽  
Lipid Composition ◽  
Unsaturated Fatty Acids ◽  
Storage Period ◽  
Total Lipid Content ◽  
Modified Atmosphere ◽  
Low Oxygen ◽  
Saturation Degree ◽  
Total Fatty Acids

AbstractApples are the most frequently consumed fruit and about 90 % of apple production is stored. Fatty acids and lipids are important constituents of plant cells. Disturbances in the lipid composition of fruit may lead to various stress processes, resulting in some storage disorders. This work is focused on an analysis of surface lipids of different varieties of apples stored in a normal atmosphere and a modified atmosphere with ultra-low oxygen content, for 4 months and 6 months. The major fatty acids in apple surface layers are palmitic acid, stearic acid, linoleic acid, and oleic acid. During the 6-months storage period, a variety-specific decrease in the total fatty acids content and an increase in saturation degree was observed in all the varieties tested, when compared with the 4-months storage. The greatest differences in saturation degree were observed in the Golden Delicious variety, in which the highest content of unsaturated fatty acids was also found. Microbial contamination of apple surfaces increased gradually over the storage process. Higher fungi levels were found in apples stored in the regular atmosphere than in the modified atmosphere, which can be attributed to changes observed in the total lipid content and saturation degree of the surface fatty acids and also to the sensitivity of microorganisms to the oxygen content in the storage room.

Regional differences in lipid composition and incorporation of saturated and unsaturated fatty acids into microsomal membranes of rat small intestine

1988 ◽  
Vol 66 (6) ◽  
pp. 794-800 ◽  
Author(s):  
M. L. Garg ◽  
M. Keelan ◽  
A. Wierzbicki ◽  
A. B. R. Thomson ◽  
M. T. Clandinin
Keyword(s):  
Fatty Acids ◽  
Small Intestine ◽  
Lipid Composition ◽  
Unsaturated Fatty Acids ◽  
Lipid Synthesis ◽  
Proximal Jejunum ◽  
Rat Small Intestine ◽  
Membrane Phospholipids ◽  
Significant Difference ◽  
Microsomal Membranes

Incorporation of [1-14C]palmitic (16:0) and [1-14C]linoleic (18:2ω6) acids into microsomal membranes of proximal (jejunum) and distal (ileum) regions of rat small intestine was investigated, and the lipid composition, including fatty acid profiles of membrane phospholipids, was determined. Jejunal microsomes contained significantly higher amounts of total phospholipids, phosphatidylcholine, and phosphatidylinositol, and lower amounts of cholesterol and sphingomyelin when compared with ileal microsomes. Jejunal microsomal phospholipids contained higher levels of stearic (18:0), 18:2ω6, and eicosapentaenoic (20:5ω3) acids followed by reduced levels of oleic (18:1ω9), arachidonic (20:4ω6), and docosahexaenoic (22:6ω3) acids when compared with those from the ileum, except for phosphatidylinositol where no significant difference between 20:4ω6 content of each site was observed. In both jejunal and ileal microsomes, incorporation of [1-14C]18:2ω6 was significantly higher than that of [1-14C]16:0. Incorporation of both [1-14C]16:0 and [1-14C]18:2ω6 was significantly higher in jejunal microsomal lipid fractions (phospholipids, diacylglycerols, triacylglycerols) when compared with the ileal microsomal fraction. These data suggest that (1) jejunal and ileal microsomal membranes differ from each other in terms of lipid composition and lipid synthesis, (2) site variations in the specificity of acyltransferases for different fatty acids exist, and (3) higher Δ9-, Δ6-, Δ5-, and Δ4-desaturase activities exist in ileal compared with jejunal enterocytes.

DEVELOPMENT OF AN EXPERIMENTAL MODEL OF AVITAMINOSIS F

2020 ◽  
Vol 20 (2) ◽  
pp. 38-40
Author(s):  
A. Levitsky ◽  
A. Lapinska ◽  
I. Selivanskaya
Keyword(s):  
Fatty Acids ◽  
Experimental Model ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Coconut Oil ◽  
The Body ◽  
Regulatory Function ◽  
Omega 3 ◽  
White Rats ◽  
Arachidonic Acids

The article analyzes the role of essential polyunsaturated fatty acids (PUFA), especially omega-3 series in humans and animals. The biosynthesis of essential PUFA in humans and animals is very limited, so they must be consumed with food (feed). Тhe ratio of omega-3 and omega-6 PUFA is very important. Biomembranes of animal cells contain about 30% PUFA with a ratio of ω-6/ ω-3 1-2. As this ratio increases, the physicochemical properties of biomembranes and the functional activity of their receptors change. The regulatory function of essential PUFA is that in the body under the action of oxygenase enzymes (cyclooxygenase, lipoxygenase) are formed extremely active hormone-like substances (eicosanoids and docosanoids), which affect a number of physiological processes: inflammation, immunity, metabolism. Moreover, ω-6 PUFA form eicosanoids, which have pro-inflammatory, immunosuppressive properties, and ω-3 PUFAs form eicosanoids and docosanoids, which have anti-inflammatory and immunostimulatory properties. Deficiency of essential PUFA, and especially ω-3 PUFA, leads to impaired development of the body and its state of health, which are manifestations of avitaminosis F. Prevention and treatment of avitaminosis F is carried out with drugs that contain PUFA. To create new, more effective vitamin F preparations, it is necessary to reproduce the model of vitamin F deficiency. An experimental model of vitamin F deficiency in white rats kept on a fat –free diet with the addition of coconut oil, which is almost completely free of unsaturated fatty acids, and saturated fatty acids make up almost 99 % of all fatty acids was developed. The total content of ω-6 PUFA (sum of linoleic and arachidonic acids), the content of ω-3 PUFA (α-linolenic, eicosapentaenoic and docosahexaenoic acids) in neutral lipids (triglycerides and cholesterol esters) defined. Тhe content of ω-6 PUFA under the influence of coconut oil decreased by 3.3 times, and the content of ω-3 PUFA - by 7.5 times. Тhe influence of coconut oil, the content of ω-6 PUFA decreased by 2.1 times, and the content of ω-3 PUFA - by 2.8 times. The most strongly reduces the content of ω-3 PUFA, namely eicosapentaenoic, coconut oil, starting from 5 %. Consumption of FFD with a content of 15 % coconut oil reduces the content of eicosapentaenoic acid to zero, ie we have an absolute deficiency of one of the most important essential PUFAs, which determined the presence of vitamin F deficiency.

scholarly journals Influence of the method of disintegration of biomass of microalga Chlorella sorokiniana on the production of lipid fraction

2019 ◽  
Vol 59 (7) ◽  
pp. 85-91
Author(s):  
Yulia A. Smyatskaya ◽  
◽  
Natalia A. Politaeva ◽  
Amira Toumi ◽  
◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Microwave Radiation ◽  
High Speed ◽  
Unsaturated Fatty Acids ◽  
Lipid Fraction ◽  
Chlorella Sorokiniana ◽  
Lipid Fractions ◽  
Significant Difference ◽  
Selection Of

This article discusses the effect of the disintegration of the cell wall of the microalgae Chlorella sorokiniana on the output of the lipid fraction. The biomass of the microalgae Chlorella sorokiniana was grown under laboratory conditions in special photobioreactors at a temperature of 25 °C, with a constant aeration of a mixture of carbon dioxide and air at a rate of 1.5 liters/min, illumination 2200-2800 Lx. Nutrient medium for cultivation contained macro – and micronutrients for high-speed growth of microalgae. Selection of optimal cultivation parameters allows obtaining biomass with desired properties. Disintegration was carried out with the homogenization of biomass and under the influence of microwave radiation. Extraction of lipids was carried out on a semi-automatic extractor according to the Randall method, using organic solvents. The output of the lipid fraction without treatment was 10.18% after the destruction of the cell wall 14.45% with the homogenization of biomass and 13.85% under the influence of microwave radiation. A qualitative analysis of the lipid fraction, carried out under gas chromatography, obtained under various conditions showed that there was no significant difference in composition from the disintegration method. Lipid fractions (more than 50%) in both cases consist mainly of unsaturated fatty acids, of which irreplaceable unsaturated fatty acids constitute more than 18% for both samples. The residual biomass formed after the extraction of the lipid fraction can be used as fertilizer in the plant, for the manufacture of sorption materials for the purification of industrial water and as a biofuel. The purpose of this study was to study the effect of cell wall disintegration on the output of the lipid fraction and qualitative composition.

scholarly journals Chemical composition of germinating Tunisian almond (Prunus Amygdalus Mill.) seedlings oil

2021 ◽  
Vol 48 (2) ◽  
Author(s):  
Refka Dhouibi ◽  
◽  
Hanen Oueslati ◽  
Senda Bahri ◽  
Khaled Jabou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Lipolytic Activity ◽  
Chemical Properties ◽  
Dry Mass ◽  
Chemical Components ◽  
Prunus Amygdalus ◽  
Almond Oil ◽  
Physico Chemical ◽  
Total Fatty Acids

Almonds (Prunus amygdalus) are a rich source of many essential nutrients. However, there is a lack of enough information on almond varieties' biochemical composition, especially at the germination stage. Therefore, this study was conducted to determine the chemical components of the germinating Tunisian almonds. The study included determining the content of oils, proteins, fatty acids, and triglycerides during germination. Results indicated that the oleaginous seeds are rich in oil (55 to 65% of the dry mass) and crude protein (21.825 mg/mL). The dominant polyunsaturated fatty acids are oleic and linoleic acids which represent 64.53% and 24.38%, respectively, while palmitic acid is the most dominant saturated fatty acid with 7.65% of the total fatty acids. Also, the primary molecular types of triglycerides detected by L.C. analysis are triolein (32.3%) and dioleolinolein (24.0%), followed by palmitodiolein (12.5%) and oleodilinolein (12.6%.). The physico-chemical properties study revealed that almond oil remains stable, thus preserving its quality and nutritional value, even during transition from dormancy to germination. On the other hand, we also detected the presence of a lipolytic activity which is maximum on the 3rd day of germination (4.66 mUI). Our results indicate that almond oil plays an important role in human nutrition due to the presence of unsaturated fatty acids, and it is more stable than other oils.

scholarly journals Effect of atorvastatin and rosuvasta tin on the fatty acid spectrum of lymphocyte membranes in patients with unstable angina

2020 ◽  
pp. 92-95
Author(s):  
T.V. Bogdan ◽  
Keyword(s):  
Cardiovascular Disease ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Unstable Angina ◽  
Unsaturated Fatty Acids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Membrane Phospholipids ◽  
Stabilization Of Membranes ◽  
Dynamic Structures

Numerous studies have demonstrated the superiority of rosuvastatin over other statins in the treatment of cardiovascular disease. It has been proven that rosuvastatin is more effectively lowers low-density lipoprotein cholesterol in patients with cardiovascular disease than other members of this drug group. Despite the known mechanisms of action of statins on blood lipids, their effective use in patients with cardiovascular disease, as well as side effects, the influence of these drugs on the fatty acid spectrum of lymphocyte (LC) membrane phospholipids in patients with ischemic heart disease remains unexplored. The results of the studies cited in the article indicate that, in patients with unstable angina who received the therapy that included rosuvastatin, unlike patients receiving the basic treatment with atorvastatin, the relative phosphate lipid contents of palmitic, stearic, and stearin arachidonic polyunsaturated fatty acids and the amount of unsaturated fatty acids are normalized, which testifies to the stabilization of membranes as dynamic structures.

Regulation Of The Fatty Acid Composition Of Platelet Phospholipids

1981 ◽  
Author(s):  
M L McKean ◽  
J B Smith ◽  
M J Silver
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Unsaturated Fatty Acids ◽  
De Novo ◽  
Membrane Phospholipids ◽  
De Novo Biosynthesis ◽  
Coa Transferase

The fatty acid composition of cell membrane phospholipids does not remain constant after de novo biosynthesis, but undergoes continual remodelling. One of the major routes for remodelling probably includes the deacylation-reacylation steps of the Lands Pathway. This has been shown to be important for the incorporation of long chain, polyunsaturated fatty acids into phospholipids by liver and brain. An understanding of the mechanisms involved in these processes in platelets is especially important in light of the large stores of arachidonic acid (AA) in platelet phospholipids and the role of AA in hemostasis and thrombosis. Previous results from this laboratory have shown that the turnover of radioactive AA, 8,11,14-eicosatrienoic and 5,8,11,14,17-eicosapentaenoic acids in the phospholipids of resting platelets is more rapid than the turnover of radioactive C16 and C18 saturated and unsaturated fatty acids. However, little is known about how fatty acids, especially AA and its homologues, are incorporated into platelet phospholipids during de novo biosynthesis or how they are exchanged during remodelling.At least three enzymes are involved in the deacylation- reacylation of phospholipids: phospholipase A2; acyl CoA synthetase; and acyl CoA transferase. We have studied acyl CoA transferase and have found considerable activity in human platelet membranes. Experiments are in progress to determine the substrate specificity and other properties of this enzyme.

scholarly journals Chlamydomonas reinhardtii Is a Potential Food Supplement with the Capacity to Outperform Chlorella and Spirulina

2020 ◽  
Vol 10 (19) ◽  
pp. 6736
Author(s):  
Randa Darwish ◽  
Mohamed A. Gedi ◽  
Patchaniya Akepach ◽  
Hirut Assaye ◽  
Abdelrahman S. Zaky ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Chlamydomonas Reinhardtii ◽  
Unsaturated Fatty Acids ◽  
Model Organism ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Food Supplement ◽  
Alpha Linolenic Acid ◽  
Total Carotenoids ◽  
Total Fatty Acids

Chlamydomonas reinhardtii is a green microalgae used as a model organism associated with biotechnological applications, yet its nutritional value has not been assessed. This study investigates the nutritional capacity of C. reinhardtii as an additional value for this species beyond its known potential in biofuels and bio-products production. The composition of key nutrients in C. reinhardtii was compared with Chlorella and Spirulina, the species widely regarded as a superfood. The results revealed that the protein content of C. reinhardtii (46.9%) was comparable with that of Chlorella (45.3) and Spirulina (50.4%) on a dry weight basis. C. reinhardtii contained all the essential amino acids with good scores based on FAO/WHO values (0.9–1.9) as in Chlorella and Spirulina. Unsaturated fatty acids predominated the total fatty acids profile of C. reinhardtii were ~74 of which ~48% are n-3 fatty acids. Alpha-linolenic acid (ALA) content in C. reinhardtii (42.4%) was significantly higher than that of Chlorella (23.4) and Spirulina (0.12%). For minerals, Spirulina was rich in iron (3.73 mg/g DW) followed by Chlorella (1.34 mg/g DW) and C. reinhardtii (0.96 mg/g DW). C. reinhardtii, unlike the other two species, consisted of selenium (10 µg/g DW), and had a remarkably lower heavy metal load. Moreover, C. reinhardtii contained relatively high concentrations of chlorophyll (a + b) and total carotenoids (28.6 mg/g DW and 6.9 mg/g DW, respectively) compared with Chlorella (12.0 mg/g DW and 1.8 mg/g DW, respectively) and Spirulina (8.6 mg/g DW and 0.8 mg/g DW, respectively). This study confirms that, based on its nutrient credentials, C. reinhardtii has great potential as a new superfood or ingredient for a food supplement.

scholarly journals Current Knowledge on Interspecific Hybrid Palm Oils as Food and Food Ingredient

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 631
Author(s):  
Massimo Mozzon ◽  
Roberta Foligni ◽  
Cinzia Mannozzi
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Elaeis Guineensis ◽  
Current Knowledge ◽  
Fats And Oils ◽  
Crude Oils ◽  
Human Consumption ◽  
Food Ingredient ◽  
Elaeis Oleifera ◽  
Total Fatty Acids

The consumers’ opinion concerning conventional palm (Elaeis guineensis) oil is negatively affected by environmental and nutritional issues. However, oils extracted from drupes of interspecific hybrids Elaeis oleifera × E. guineensis are getting more and more interest, due to their chemical and nutritional properties. Unsaturated fatty acids (oleic and linoleic) are the most abundant constituents (60%–80% of total fatty acids) of hybrid palm oil (HPO) and are mainly acylated in position sn-2 of the glycerol backbone. Carotenes and tocotrienols are the most interesting components of the unsaponifiable matter, even if their amount in crude oils varies greatly. The Codex Committee on Fats and Oils recently provided HPO the “dignity” of codified fat substance for human consumption and defined the physical and chemical parameters for genuine crude oils. However, only few researches have been conducted to date on the functional and technological properties of HPO, thus limiting its utilization in food industry. Recent studies on the nutritional effects of HPO softened the initial enthusiasm about the “tropical equivalent of olive oil”, suggesting that the overconsumption of HPO in the most-consumed processed foods should be carefully monitored.

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