Competition among Rhizobium leguminosarum bv. phaseoli strains for nodulation of common bean

1992 ◽  
Vol 38 (2) ◽  
pp. 157-160 ◽  
Author(s):  
Maria Luz C. George ◽  
Françoise M. Robert
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Key Words ◽  
Rhizobium Leguminosarum ◽  
Nodule Occupancy ◽  
Phaseolus Vulgaris L ◽  
Ideal Model ◽  
Genetic Studies ◽  
Bush Bean ◽  
Nodulation Competitiveness

Six effective Rhizobium leguminosarum bv. phaseoli strains were examined for nodulation competitiveness on common bean (Phaseolus vulgaris L.), using all possible two-strain combinations of inoculum. Nodule occupancy was determined with strain-specific fluorescent antibodies. The strains were divided into three groups according to their overall competitive abilities on pole bean cv. Kentucky Wonder and bush bean cv. Bountiful. Strains TAL 182 and TAL 1472 were highly competitive (greater than 70% nodule occupancy); strains KIM-5, Viking 1, and CIAT 899 were moderately competitive (approximately 50% nodule occupancy); and strain CIAT 632 was poorly competitive (less than 5% nodule occupancy). The competitiveness of the six strains was similar on the two host cultivars. The proportion of competing strains in the inoculum influenced the nodule occupancy of the highly competitive and moderately competitive strains, but not that of the poorly competitive strain. Two outstanding strains (TAL 182 and TAL 1472) were identified as ideal model strains for molecular and genetic studies on nodulation competitiveness. Key words: Rhizobium, Phaseolus vulgaris, nodulation, competition.

scholarly journals Heterosis for the root distribution trait in common bean

2020 ◽  
Vol 43 ◽  
pp. e46712
Author(s):  
Sibila Grigolo ◽  
Rita Carolina de Melo ◽  
Ana Carolina da Costa Lara Fioreze ◽  
Altamir Frederico Guidolin ◽  
Jefferson Luís Meirelles Coimbra
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Abiotic Stresses ◽  
Gene Pool ◽  
Root Distribution ◽  
Environmental Changes ◽  
Phaseolus Vulgaris L ◽  
Epistatic Interactions ◽  
Genetic Studies ◽  
The Mean

Heterosis has been one of the most studied phenomena since genetic studies were conceived. Knowledge of this phenomenon for the root distribution trait in beans (Phaseolus vulgaris L.) can favour the development of genotypes that are more tolerant to abiotic stresses. This study aimed to verify the occurrence of heterosis for the root distribution trait in hybrid F1 populations of common bean derived from crosses within and between gene groups. Thirty-six populations (six parents and 30 hybrids) were evaluated under field conditions in the agricultural years 2016/17 and 2017/18. The root distribution was assessed with the adapted Bohm method. The genotypes responded similarly to environmental changes (absence of an interaction). However, the root distribution differed significantly between the agricultural years. Comparisons between the mean of hybrids from crosses within each gene pool and the mean of parents did not reveal heterosis. The comparisons between gene groups showed significant differences only between BRS Embaixador x IPR Uirapuru, CBS 14 x IPR Uirapuru, and BAF 53 x IPR Uirapuru and the mean of parents. The absence of heterosis in the root trait can be explained by the degree of kinship between the gene groups, as well as some epistatic interactions.

Comparison of growth and N accumulation of common bean (Phaseolus vulgaris L.) cv. OAC Rico and its two nodulation mutants, R69 and R99

1996 ◽  
Vol 76 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Steven J. Shirtliffe ◽  
J. Kevin Vessey ◽  
B. R. Buttery ◽  
S. J. Park
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rhizobium Leguminosarum ◽  
Field Measurements ◽  
Wild Type ◽  
Phaseolus Vulgaris L ◽  
Mineral N ◽  
N Accumulation ◽  
Nodulation Mutants ◽  
Wild Type Parent

Nodulation mutants are present in many species of legumes and have potential as ideal reference crops in field measurements of N2 fixation. The objective of this experiment was to characterize the growth, development, mineral-N accumulation, and N2 fixation of two nodulation mutants of common bean (Phaseolus vulgaris L.), R69 (nod+/fix−) and R99 (nod−) in comparison with the wild type parent OAC Rico. The mutants were incapable of N2 fixation. With dual dependence on N2 fixation and [Formula: see text] as N sources, OAC Rico accumulated more N than R69 and R99. However, when supplied with mineral [Formula: see text] sufficient to prevent N2 fixation in OAC Rico, its growth and N accumulation did not differ from those of the mutants. There were differences in internode length that resulted in different heights among the three lines. The addition of silver to the nutrient solution failed to restore nodulation to R99. As a result, it was determined that the nod− phenotype is not a result of an ethylene-related mutation. The nodulation mutants R69 and R99 appear to be good choices as reference crops for common bean in N2 fixation studies. Key words: Common bean, N accumulation, N2 fixation, nodulation mutants, Phaseolus vulgaris, Rhizobium leguminosarum bv. phaseoli

RESPONSES OF FIELD BEAN (Phaseolus vulgaris L.) TO LEVELS OF Rhizobium leguminosarum bv. phaseoli INOCULATION IN SOILS CONTAINING EFFECTIVE R. leguminosarum bv. phaseoli POPULATIONS

1989 ◽  
Vol 69 (2) ◽  
pp. 419-426 ◽  
Author(s):  
R. M. N. KUCEY
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizobium Leguminosarum ◽  
Control Plant ◽  
Significant Proportion ◽  
Field Bean ◽  
Nodule Occupancy ◽  
Phaseolus Vulgaris L ◽  
Mineral N ◽  
N Accumulation ◽  
Antibiotic Resistant

Greenhouse studies were conducted to determine the effect of adding Rhizobium leguminosarum bv. phaseoli inocula to field beans (Phaseolus vulgaris L.) growing in soils already containing R. leguminosarum bv. phaseoli. Indigenous R. leguminosarum bv. phaseoli populations in the 12 soils used ranged from 1.1 × 101 to 4 × 105 rhizobia g−1 of soil. Antibiotic-resistant isolates of R. leguminosarum bv. phaseoli strain 3644 were used as inocula and inoculum levels ranged from 104 to 108 bacteria per seed. N-15 isotope dilution methods with barley as a nonfixing control plant were used to determine N2 fixation levels. Bean plants grown in soils containing greater than 8 × 10 R. leguminosarum bv. phaseoli did not show positive responses to added rhizobia, except in one soil where the inoculum formed a significant proportion of nodules on inoculated plants. Plants growing in soils with less than 8 × 103R. leguminosarum bv. phaseoli did show increased levels of plant N accumulation and dry matter production in response to rhizobium addition if the level of soil mineral N was less than 25 μg N g−1 soil. Nodule occupancy by the marked R. leguminosarum bv. phaseoli isolate increased only in soils containing 8 × 103R. leguminosarum bv. phaseoli or less. The resident population of rhizobia in many of the soils was determined to be effective in N2 fixation since the proportion of N derived from N2 fixation did not increase in response to inoculation. Increasing the number of R. leguminosarum bv. phaseoli added per seed from 104 to 108 did not generally increase the effectiveness of the added inocula. Responses of beans to R. leguminosarum bv. phaseoli inoculation can only be expected in soils with low levels of resident R. leguminosarum bv. phaseoli and mineral N.Key words: Field bean, nodule occupancy, N-15 dilution, competition, N2 fixation

Harowood common bean

1991 ◽  
Vol 71 (4) ◽  
pp. 1143-1145
Author(s):  
S. J. Park
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Key Words ◽  
Growth Habit ◽  
Bean Common Mosaic Virus ◽  
Phaseolus Vulgaris L ◽  
Plant Type ◽  
Navy Bean ◽  
Cultivar Description ◽  
Narrow Row

Harowood is a late-maturing, high-yielding white (navy) bean (Phaseolus vulgaris L.) cultivar with a semideterminate growth habit. The main advantages of Harowood over other cultivars are its more erect plant type which forms a narrow canopy and its high podding nodes which make the crop suitable for narrow row production and direct combining. Harowood is resistant to the alpha and delta races of anthracnose and to races 1 and 15 of bean common mosaic virus. Key words: Phaseolus vulgaris L., dry edible (navy, pea) bean, upright plant type, cultivar description

Preference in the nodulation ofPhaseolus vulgariscv. RAB39. II. Effect of delayed inoculation or low cell representation in the inoculant on nodule occupancy byRhizobium tropiciUMR1899

1996 ◽  
Vol 42 (8) ◽  
pp. 844-850 ◽  
Author(s):  
Constanza Montealegre ◽  
Peter H. Graham
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Root Tip ◽  
Nodule Occupancy ◽  
Phaseolus Vulgaris L ◽  
Rhizobium Tropici ◽  
Split Root ◽  
Split Root System ◽  
Marked Preference ◽  
Inoculant Strain

Common bean (Phaseolus vulgaris L.) is a traditional crop in much of Latin America, where it is often planted into soils containing numerous, sometimes ineffective, indigenous rhizobia. The presence of these indigenous organisms can limit response to inoculation. Because of this, we have sought bean cultivars that will nodulate preferentially with the inoculant strain, and have previously reported on the preference between the bean cultivar RAB39 and strains of Rhizobium tropici. We have detailed this interaction using the inoculant-quality strain UMR1899. In the present study the root tip marking (RTM) technique was used to demonstrate that this preference in nodulation was evident, even when inoculation with UMR1899 was delayed up to 8 relative to that with Rhizobium etli UMR1632. In contrast to studies with other legumes, roots of RAB39 were not predisposed to nodulate with UMR1632, even though preexposed to this strain for considerable periods of time. The presence of UMR1899 actually reduced nodulation by UMR1632 substantially, even when inoculation with UMR1899 was significantly delayed. When UMR1899 and UMR1632 were applied to separate halves of a split-root system, the number of nodules on the side receiving UMR1632 was less than for the half root inoculated with UMR1899, but the differences were not significant. This suggests that the preference response is not systemic but requires proximity between the strains involved. UMR1899 produced more than 50% of the nodules even when the ratio of UMR1632:UMR1899 in the inoculant was 10:1. The results are further evidence of a stable and marked preference of RAB39 for UMR1899, which warrants a more detailed study at the field level.Key words: Phaseolus vulgaris L., common bean, delayed inoculation, strain preference, cell proportions.

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