Promiscuous nodulation of Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala by indigenous Rhizobium meliloti

1990 ◽  
Vol 36 (5) ◽  
pp. 369-372 ◽  
Author(s):  
E. S. P. Bromfield ◽  
L. R. Barran
Keyword(s):  
Phaseolus Vulgaris ◽  
Host Range ◽  
Leucaena Leucocephala ◽  
Rhizobium Meliloti ◽  
Symbiotic Genes ◽  
Apparent Relationship ◽  
Macroptilium Atropurpureum ◽  
Axenic Conditions ◽  
Cryptic Plasmids ◽  
Reference Strains

Thirty-three isolates of indigenous Rhizobium meliloti, either possessing cryptic plasmids that hybridize to probes for symbiotic genes or lacking a 1500-kb megaplasmid band in Eckhardt gels, were tested for infectivity on 10 legume species grown under axenic conditions. A previous study had shown that all but two isolates were symbiotically effective with Medicago sativa. All indigenous isolates and two reference strains of R. meliloti induced nodules which were symbiotically ineffective on Trigonella foenum-graecum (100% plants nodulated) and Phaseolus vulgaris (40 to 100% plants nodulated). Eighteen indigenous isolates of R. meliloti elicited ineffective nodules on Macroptilium atropurpureum (2 to 25% plants nodulated) and Leucaena leucocephala (11 to 75% plants nodulated). The identity of single colony nodule isolates from each R. meliloti inoculant and host combination was verified by phage typing and analysis of plasmid profiles; tests with subsamples of these isolates showed that all were capable of nodulating M. sativa. There was no apparent relationship between the host range of indigenous R. meliloti and either the presence of cryptic plasmids that hybridize to probes for symbiotic genes or the absence of a megaplasmid band in Eckhardt gels. The data suggest that nodulation promiscuity may be a relatively common characteristic of R. meliloti. Key words: host range, Rhizobium meliloti, Leucaena, Macroptilium, Phaseolus.

scholarly journals Sulfation of Nod Factors via nodHPQ Is nodD Independent in Rhizobium tropici CIAT899

1998 ◽  
Vol 11 (10) ◽  
pp. 979-987 ◽  
Author(s):  
Jorge Luis Folch-Mallol ◽  
Hamid Manyani ◽  
Silvia Marroquí ◽  
Carolina Sousa ◽  
Carmen Vargas ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Leucaena Leucocephala ◽  
Regulatory Region ◽  
Constitutive Expression ◽  
Nod Factors ◽  
Rhizobium Tropici ◽  
Independent Manner ◽  
Macroptilium Atropurpureum ◽  
Tlc Analysis ◽  
Layer Chromatography

A cosmid from the Rhizobium tropici CIAT899 symbiotic plasmid, containing most of the nodulation genes described in this strain, has been isolated. Although this cosmid does not carry a nodD gene, it confers ability to heterologous Rhizobium spp. to nodulate R. tropici hosts (Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala). The observed phenotype is due to constitutive expression of the nodABCSUIJ operon, which has lost its regulatory region and is expressed from a promoter present in the cloning vector. Thin-layer chromatography (TLC) analysis of the Nod factors produced by this construction shows that it is still capable of synthesizing sulfated compounds, suggesting that the nodHPQ genes are organized as an operon that is transcribed in a nodD-independent manner and is not regulated by flavonoids. Se ha aislado un cósmido del plásmido simbiótico de Rhizobium tropici CIAT899 que contiene la mayoría de los genes de nodulación descrito para esta estirpe, menos el gen regulador nodD. La introducción de este cósmido en una estirpe curada del plásmido simbiótico de R. tropici CIAT899 permite la nodulación en las plantas ensayadas (Phaseolus vulgaris, Macroptilium atropurpureum, y Leucaena leucocephala). El fenotipo observado se debe a la expresión constitutiva del operón nodABCSUIJ bajo el promotor del gen de resistencia a la kanamicina, que lleva el vector donde se ha clonado el fragmento de ADN. Análisis por cromatografia de capa fina demuestran que esta construcción es capaz de sulfatar el extremo reductor del factor Nod. Estas evidencias sugieren que los genes nodHPQ constituyen un operón, y que su expresión es independiente del gen regulador nodD.

scholarly journals Avaliação quantitativa e qualitativa da fitomassa de leguminosas para uso como cobertura de solo

2004 ◽  
Vol 34 (3) ◽  
pp. 947-949 ◽  
Author(s):  
João Tavares Nascimento ◽  
Ivandro de França da Silva
Keyword(s):  
Leucaena Leucocephala ◽  
Cajanus Cajan ◽  
Pueraria Phaseoloides ◽  
Crotalaria Juncea ◽  
Clitoria Ternatea ◽  
Canavalia Ensiformis ◽  
Calopogonium Mucunoides ◽  
Macroptilium Atropurpureum

Para uso como adubo verde em um experimento em Alagoinha-PB, avaliaram-se doze espécies de leguminosas: crotalária (Crotalaria juncea L), guandu (Cajanus cajan L), guandu anão (Cajanus cajan (L) Millsp), calopogônio (Calopogonium mucunoides L), feijão-de-porco (Canavalia ensiformis L), lab-lab (Dolichos lab lab L), kudzu tropical (Pueraria phaseoloides L), siratro (Macroptilium atropurpureum L), leucena (Leucaena leucocephala L), cunhã (Clitoria ternatea L), mucuna preta (Styzolobium aterrimum L), e mucuna cinza (Styzolobium cinereum Piper e Tracy), no DBC com 12 tratamentos, em parcelas de 20m2, com três repetições. Constataram-se as maiores produções de fitomassa para leucena, guandu, mucuna preta, kudzu tropical, feijão-de-porco e cunhã, enquanto que para a crotalária, a menor produção. A fitomassa do kudzu tropical mostrou-se com melhor qualidade para a incorporação.

scholarly journals Un sistema agroforestal de secano para el altiplano semiárido de México

2019 ◽  
pp. 89-103
Author(s):  
Esteban Salvador Osuna-Ceja ◽  
Benjamín Figueroa-Sandoval ◽  
Miguel Ángel Martínez-Gamiño ◽  
José Pimentel-López
Keyword(s):  
Phaseolus Vulgaris ◽  
Sorghum Bicolor ◽  
Leucaena Leucocephala ◽  
Phaseolus Vulgaris L ◽  
Sorghum Bicolor L

En el Altiplano semiárido del centro-norte de México, la erosión del suelo es uno de los principales problemas que afectan la sustentabilidad de las tierras agrícolas. Como resultado, los rendimientos y los ingresos son bajos y la calidad del suelo continúa disminuyendo. Con el objetivo de desarrollar un sistema de explotación agroforestal para el manejo sostenible de suelos, se diseñó un sistema agroforestal (SAF) de secano que incluyó sorgo (Sorghum bicolor L. Moench) de nervadura café (bmr) y frijol (Phaseolus vulgaris L.) en rotación, nopal (Opuntia sp.) y leucaena (Leucocephala glauca) como alternativa de manejo mejorado de las unidades de producción agrícola, y no como una variante más de la reconversión productiva. El SAF se evalúo en el Sitio Experimental ‘Sandovales’, Aguascalientes, durante 2014 y 2015, en condiciones ambientales restrictivas, con 344 y 320 mm de precipitación en los ciclos de cultivo para probar que es sostenible, pues favorece la conservación y fertilidad del suelo y el aprovechamiento del agua de lluvia. El rendimiento promedio de sorgo sembrado en cama con seis hileras fue 6.33 t ha-1 de MS, el del frijol de las variedades Flor de Junio Dalia, Pinto Saltillo, Pinto Centenario, Flor de Mayo Dolores y Azufrado 2, en cuatro hileras fue 2.03, 1.8, 1.74, 1.34 y 0.60 t ha-1 de grano. Se concluye que es posible implementar un SAF de productividad sustentable, donde el sorgo y el frijol de secano sean incluidos en una rotación de cultivos. El SAF propuesto ofrece opciones técnicamente eficientes para el control de la erosión en la agricultura de secano, con productividad baja.

Phaseolus vulgaris is nodulated in northern Spain by Rhizobium leguminosarum strains harboring two nodC alleles present in American Rhizobium etli strains: biogeographical and evolutionary implications

2010 ◽  
Vol 56 (8) ◽  
pp. 657-666 ◽  
Author(s):  
Paula García-Fraile ◽  
Daniel Mulas-García ◽  
Alvaro Peix ◽  
Raúl Rivas ◽  
Fernando González-Andrés ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizobium Leguminosarum ◽  
Intergenic Spacer ◽  
Climatic Conditions ◽  
Phylogenetic Group ◽  
Rhizobium Etli ◽  
Symbiotic Genes ◽  
Northern Spain ◽  
Major Producer ◽  
Core Genes

In this study a collection of rhizobial strains were isolated from effective nodules of Phaseolus vulgaris in a wide region of northern Spain, which is the major producer region of this legume in Spain. The analysis of their core genes, rrs, atpD, and recA, and the 16S–23S intergenic spacer showed that all isolates belong to the phylogenetic group of Rhizobium leguminosarum and some of them were identical to those of strains nodulating Vicia or Trifolium . None of the isolates was identified as Rhizobium etli ; however, all of them carry the nodC alleles α and γ harboured by American strains of this species. These alleles were also found in strains nodulating P. vulgaris in southern Spain identified as R. etli. These results suggest that R. etli was carried from America to Spain with common bean seeds, but that they could have found difficulties persisting in the soils of northern Spain, probably because of the climatic conditions. The symbiotic genes of this species could have been transferred, after the arrival of P. vulgaris, to strains of R. leguminosarum already present in northern Spanish soils.

A cryptic plasmid of indigenous Rhizobium meliloti possesses reiterated nodC and nifE genes and undergoes DNA rearrangement

1992 ◽  
Vol 38 (6) ◽  
pp. 563-568 ◽  
Author(s):  
V. K. Rastogi ◽  
E. S. P. Bromfield ◽  
S. T. Whitwill ◽  
L. R. Barran
Keyword(s):  
Genomic Dna ◽  
Rhizobium Meliloti ◽  
Phage Type ◽  
Cryptic Plasmid ◽  
Dna Rearrangement ◽  
Homologous Sequences ◽  
Phage Types ◽  
Phage Sensitivity ◽  
Cryptic Plasmids ◽  
Free Strain

Indigenous Rhizobium meliloti were previously characterized on the basis of plasmid profiles and phage sensitivity patterns (phage types). Rhizobium meliloti 1076, which contained two cryptic plasmids, was one of four isolates comprising phage type 23. In this study, the large cryptic plasmid pVS1(size >500 b) was transferred from isolate 1076 into the plasmid-free strain of Agrobacterium tumefaciens UBAPF1. This plasmid contained nucleotide sequences homologous to genes for nodulation (nodB, nodC) and nitrogen fixation (nifH, nifD, nifK, nifE). Cosmid clones possessing the nod and nif homologous sequences, which had been selected from a genomic bank of A. tumefaciens UBAPF1 containing pVS1, complemented R. meliloti nodC and nifE mutants, respectively. These results demonstrate that the nodC and nifE homologous sequences are functionally expressed. Three of four isolates comprising phage type 23 possessed a megaplasmid band in agarose gels characteristic of R. meliloti, as well as two cryptic plasmids. The fourth isolate (No. 323) lacked the large cryptic plasmid corresponding to pVS1, but instead showed a band of lesser mobility than that of the megaplasmids. Nevertheless, its restricted genomic DNA retained the nodC and nifE hybridizing fragments characteristic of pVS1, indicating that the cryptic plasmid has undergone DNA rearrangement. Key words: Rhizobium, plasmid, reiteration, genes, rearrangement.

Stability and transmissibility of the cryptic plasmids of Rhizobium meliloti GR4

1993 ◽  
Vol 160 (6) ◽  
pp. 477-485 ◽  
Author(s):  
Jes�s Mercado-Blanco ◽  
Jos� Olivares
Keyword(s):  
Rhizobium Meliloti ◽  
Cryptic Plasmids
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