Detection and quantitation of human enteric viruses in waste waters: increased sensitivity using a human immune serum globulin–immunoperoxidase assay on MA-104 cells

1987 ◽  
Vol 33 (6) ◽  
pp. 568-570 ◽  
Author(s):  
Pierre Payment ◽  
Michel Trudel
Keyword(s):  
Cytopathic Effect ◽  
Enteric Viruses ◽  
Immune Serum ◽  
Secondary Effluent ◽  
Immunoperoxidase Method ◽  
Waste Waters ◽  
Immune Serum Globulin ◽  
Serum Globulin ◽  
Human Immune ◽  
Human Enteric Viruses

This study demonstrates that the most sensitive method for the detection and quantitation of cultivable human enteric viruses in water samples after repassage in the MA-104 cell line is the detection of infected cells by the human immune serum globulin–immunoperoxidase (HISG–IP) method recently described by the authors. This immunoperoxidase method is up to 50 times more sensitive than a liquid overlay assay by cytopathic effect in BGM cells. The viral content of waste waters was evaluated with this new methodology. By this method the average viral content of raw sewage (RS) was 900 mpniu/L (most probable number of infectious units per litre), 1056 mpniu/L in primary effluent (PE), and 106 mpniu/L in secondary effluent (SE). With a cytopathic effect assay on BGM cells, values of 85 (RS), 56 (PE), and 2 (SE) mpniu/L were observed, a striking underestimation of the viral content of secondary effluents.

scholarly journals Survival of Oral Human Immune Serum Globulin in the Gastrointestinal Tract of Low Birth Weight Infants

1981 ◽  
Vol 15 (9) ◽  
pp. 1256-1260 ◽  
Author(s):  
Paul M Blum ◽  
Dale L Phelps ◽  
Bonnie J Ank ◽  
Herbert J Krantman ◽  
E Richard Stiehm
Keyword(s):  
Birth Weight ◽  
Gastrointestinal Tract ◽  
Low Birth Weight ◽  
Immune Serum ◽  
Immune Serum Globulin ◽  
Serum Globulin ◽  
Low Birth Weight Infants ◽  
Human Immune

Column Ion Exchange Chromatographie Production of Human Immune Serum Globulin for Intravenous Use

Vox Sanguinis ◽  
1985 ◽  
Vol 48 (4) ◽  
pp. 201-212
Author(s):  
A.D. Friesen ◽  
J.M. Bowman ◽  
W.C.H. Bees
Keyword(s):  
Ion Exchange ◽  
Immune Serum ◽  
Immune Serum Globulin ◽  
Serum Globulin ◽  
Human Immune

Standard and Special Human Immune Serum Globulins as Therapeutic Agents

PEDIATRICS ◽  
1979 ◽  
Vol 63 (2) ◽  
pp. 301-319
Author(s):  
E. Richard Stiehm
Keyword(s):  
Active Immunization ◽  
Immune Serum ◽  
Secretory Iga ◽  
Passive Immunity ◽  
Side Reactions ◽  
Antibody Activity ◽  
Immune Serum Globulin ◽  
Serum Globulin ◽  
Sensitivity Testing ◽  
Human Immune

Human γ-globulin is a valuable and frequently prescribed biological used to provide passive immunity against microbial agents, toxins, or cells. γ-Globulin is most widely used for the short-term prevention of certain bacterial or viral diseases when active immunization is unavailable (eg, hepatitis A) or when active immunization has not been given before exposure (eg, tetanus, rabies). γ-Globulin is also indicated on a continuous basis in replacement therapy for persons with antibody immunodeficiency. It is also (mis)used by many physicians for colds, allergies, and recurrent infection despite lack of proved efficacy. Two types of γ-globulin preparations are available: standard human immune serum globulin (HISG) for general use, and special human immune serum globulins with a known antibody content for specific illnesses (Table I). In addition, certain animal sera and antitoxins are available for passive immunity (Table I). Although a discussion of these agents is beyond the scope of this review, it is important to emphasize that their use is associated with considerable risk of adverse side reactions (anaphylaxis and serum sickness). Accordingly, the physician should know the exact indications for their use, always institute sensitivity testing, and be ready to treat side reactions immediately.1 SOME PROPERTIES OF IgG GLOBULIN The immunoglobulins or "γ-globulins" are the proteins of the plasma and tissue made in lymphoreticular tissues which have antibody activity.2 Although there are six classes of immunoglobulin—IgG, IgM, IgA, IgD, IgE, and secretory IgA—only IgG is present in significant quantities in HISG. IgG is a glycoprotein with a molecular weight of 150,000 daltons distributed equally between the serum and the tissues.

scholarly journals Prevention of non-A, non-B, post-transfusion hepatitis with intravenous administration of human immune serum globulin (Venoglobulin-I).

Kanzo ◽  
1989 ◽  
Vol 30 (8) ◽  
pp. 841-849
Author(s):  
Toshiharu SHIINO ◽  
Shigeru TAKAMOTO ◽  
Kuniaki UEDA ◽  
Noboru MIYAGAWA ◽  
Akira MURAKAMI ◽  
...  
Keyword(s):  
Intravenous Administration ◽  
Immune Serum ◽  
Immune Serum Globulin ◽  
Serum Globulin ◽  
Post Transfusion Hepatitis ◽  
Human Immune
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