Relationship between the ectomycorrhizal fungus Pisolithus tinctorius associated with loblolly pine and acid-generating Thiobacillus spp. on an acidic strip mine site

1985 ◽  
Vol 31 (9) ◽  
pp. 878-879 ◽  
Author(s):  
James W. Hendrix ◽  
Cathy Stevens Hunt ◽  
Dale M. Maronek
Keyword(s):  
Loblolly Pine ◽  
Thiobacillus Ferrooxidans ◽  
Bacterial Species ◽  
Ectomycorrhizal Fungus ◽  
Pisolithus Tinctorius ◽  
Ferrous Ions ◽  
Mine Site ◽  
Root Zones ◽  
Pine Seedlings ◽  
Strip Mine

Loblolly pine seedlings were transplanted onto a pyritic coal mine site in a commercial reclamation effort. After 5 years, trees which became naturally infected with Pisolithus tinctorius, or other ectomycorrhizal fungi which produced sporocarps, were twice the height and stem diameter of trees not associated with sporocarps. The hypothesis that P. tinctorius promotes superior growth of its host by inhibiting the growth or activity of Thiobacillus ferrooxidans and T. thiooxidans, which catalyze production of sulfuric acid from pyrite, was examined. Soil pH was lower in the root zones of trees associated with P. tinctorius than with trees not associated with P. tinctorius, and sulfate was higher. Differences were not found in ferric or ferrous ions or in populations of the two bacterial species. Apparently P. tinctorius benefits its hosts by mechanisms other than inhibition of Thiobacillus spp.

Inoculation of Nursery Seedbeds with Pisolithus Tinctorius Spores Mixed with Hydromulch Increases Ectomycorrhizae and Growth of Loblolly Pines

1979 ◽  
Vol 3 (4) ◽  
pp. 175-178 ◽  
Author(s):  
Donald H. Marx ◽  
John G. Mexal ◽  
William G. Morris
Keyword(s):  
Loblolly Pine ◽  
Pisolithus Tinctorius ◽  
Pine Seedlings ◽  
Loblolly Pines

Abstract Different methods of introducing basidiospores (4/5 oz. spores/100 linear ft. of nursery bed) of Pisolithus tinctorius into fumigated soil at Weyerhaeuser's nursery in Oklahoma were tested to determine their effectiveness in forming ectomycorrhizae on loblolly pine seedlings. Two of five methods proved significantly effective. Nearly three-fourths of seedlings treated with spores mixed in hydromulch and applied after sowing formed Pisolithus ectomycorrhizae. The result was 25 percent larger seedlings and 15 percent fewer culls. In plots where spores were dusted onto the soil at sowing, one-third of the seedlings formed Pisolithus ectomycorrhizae, resulting in 12 percent larger seedlings and 13 percent fewer culls.

scholarly journals Accumulation and localization of aluminium in root tips of loblolly pine seedlings and the associated ectomycorrhiza Pisolithus tinctorius

2005 ◽  
Vol 28 (2) ◽  
pp. 111-120 ◽  
Author(s):  
K. MOYER-HENRY ◽  
I. SILVA ◽  
J. MACFALL ◽  
E. JOHANNES ◽  
N. ALLEN ◽  
...  
Keyword(s):  
Loblolly Pine ◽  
Pisolithus Tinctorius ◽  
Root Tips ◽  
Pine Seedlings

scholarly journals Successful Reforestation of South Carolina Sandhills is not influenced by Seedling Inoculation with Pisolithus tinctorius in the Nursery

1999 ◽  
Vol 23 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Michelle M. Cram ◽  
John G. Mexal ◽  
Ray Souter
Keyword(s):  
South Carolina ◽  
Loblolly Pine ◽  
Growth Response ◽  
Longleaf Pine ◽  
Pinus Palustris ◽  
Pisolithus Tinctorius ◽  
Savannah River ◽  
Pine Seedlings ◽  
Seedling Inoculation ◽  
River Site

Abstract Longleaf (Pinus palustris) and loblolly pine (P. taeda) nursery beds were treated with either vegetative inoculum of Pisolithus tinctorius (Pt) or allowed to become inoculated with natural ectomycorrhizae (NI) from 1987 to 1991. The resulting Pt and NI seedlings were outplanted on 2 sites per year (1988-1992) for a total of 10 demonstration plantings on the Savannah River Site (SRS), South Carolina. After 4 yr (5 yr for Site 5) the presence of Pt on bareroot longleaf pine at the time of planting did not improve seedling diameter or height growth. Longleaf pine survival was increased with the presence of Pt only on one site, while NI seedlings had greater survival on five sites and greater growth on two sites. The presence of Pt on the containerized longleaf pine had no effect on survival or diameter growth after 4 yr. Inoculation of bareroot loblolly pine with Pt resulted in larger diameter seedlings at the time of planting for four of eight sites. However, these differences in diameter were not maintained in three of the sites after 4 yr. The use of Pt as an artificial inoculant of bareroot longleaf and loblolly pine seedlings does not increase survival or growth response of reforestation plantings on the sandhills of South Carolina after 4 (5) yr. South. J. Appl. For. 23(1):46-52.

Early Field Performance of Loblolly Pine Seedlings with Pisolithus Tinctorius Ectomycorrhizae on Two Lower Coastal Plain Sites

1983 ◽  
Vol 7 (3) ◽  
pp. 149-153 ◽  
Author(s):  
Gregory N. Leach ◽  
Homer H. Gresham
Keyword(s):  
Loblolly Pine ◽  
Coastal Plain ◽  
Field Performance ◽  
Pisolithus Tinctorius ◽  
Growing Seasons ◽  
Pine Seedlings ◽  
Bare Root ◽  
Pinus Taeda L ◽  
Lower Coastal Plain ◽  
Growth Of Seedlings

Abstract Bare root seedlings of loblolly pine (Pinus taeda L.) with Pisolithus tinctorius (Pers.) Coker and Couch (Pt) ectomycorrhizae were outplanted on typical reforestation sites in the lower coastal plain in Florida and Georgia. After two growing seasons, survival and height growth of seedlings with heavy Pt colonization at planting were not significantly different from control seedlings naturally infected with other ectomycorrhizae. Mycelial inoculum added to the nursery soil resulted in greater Pt colonization of seedlings than basidiospore inoculum applied in an inert coating on encapsulated seed.

Influence of Fertilization and Ectomycorrhizae on Loblolly Pine Growth and Susceptibility to Fusiform Rust

1983 ◽  
Vol 7 (2) ◽  
pp. 101-103 ◽  
Author(s):  
H. R. Powers ◽  
S. J. Rowan
Keyword(s):  
Loblolly Pine ◽  
Cultural Practices ◽  
Mycorrhizal Fungus ◽  
Height Growth ◽  
Pisolithus Tinctorius ◽  
Fusiform Rust ◽  
Infected Tree ◽  
Pine Seedlings ◽  
Susceptible Control ◽  
Rust Severity

Abstract The application of fertilizer and the mycorrhizal fungus, Pisolithus tinctorius, did not increase the height growth of loblolly pine seedlings during their first four years of growth. Fertilization, however, did increase the overall number of fusiform rust galls per infected tree. This increase was highly significant on the susceptible control seedlings, but was not significant on resistant Livingston Parish seedlings. This indicates that cultural practices such as fertilization can be applied to rust-resistant seedlings, such as the Livingston Parish seed source, without significantly increasing rust severity.

Ectomycorrhizal Inoculation of Containerized Douglas-fir and Lodgepole Pine Seedlings with Six Isolates of Pisolithus tinctorius

1979 ◽  
Vol 25 (4) ◽  
pp. 585-590
Author(s):  
Randy Molina
Keyword(s):  
Lodgepole Pine ◽  
Ectomycorrhizal Fungus ◽  
Douglas Fir ◽  
Pisolithus Tinctorius ◽  
Seedling Height ◽  
Vegetative Mycelium ◽  
Pine Seedlings ◽  
Specific Strain ◽  
Wide Scale ◽  
Inoculation Treatment

Abstract Containerized Douglas-fir and lodgepole pine seedlings grown under lowered fertility were inoculated with vegetative mycelium from six isolates of the ectomycorrhizal fungus Pisolithus tinctorius. The isolates differed both culturally and in effectiveness as ectomycorrhizal inoculum. The percent of seedlings successfully inoculated and percent of feeder roots colonized differed significantly among the isolates. Analysis of variance, however, for seedling height, stem diameter, stem and root dry weights, and top/root ratio showed no significant differences between any inoculation treatment and controls for either tree species. This study reinforces the concept that a wide array of fungus ecotypes should be tested before a specific strain is selected for wide-scale nursery inoculations. Forest Sci. 25:585-590.

scholarly journals Role of Suillus placidus in Improving the Drought Tolerance of Masson Pine (Pinus massoniana Lamb.) Seedlings

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 332
Author(s):  
Min Li ◽  
Haoyun Wang ◽  
Xizhou Zhao ◽  
Zhongke Lu ◽  
Xueguang Sun ◽  
...  
Keyword(s):  
Drought Stress ◽  
Defense Mechanism ◽  
Southern China ◽  
Dry Weight ◽  
Pinus Massoniana ◽  
Photosynthetic Performance ◽  
Ectomycorrhizal Fungus ◽  
Severe Drought ◽  
Masson Pine ◽  
Pine Seedlings

Masson pine is an important afforestation species in southern China, where seasonal drought is common. The present study focused on the effects of Suillus placidus, an ectomycorrhizal fungus, inoculation on the growth and physiological and biochemical performance of masson pine seedlings under four different watering treatments (well-watered, mild drought, moderate drought, and severe drought) to evaluate the symbiotic relationship between S. placidus and masson pine seedlings. Ectomycorrhizal-inoculated (ECM) and non-inoculated (NM) seedlings were grown in pots and maintained for 60 days using the weighing method. Results showed that seedlings’ growth, dry weight, RWC, chlorophyll content, PSII efficiency, and photosynthesis decreased as drought stress intensified in both ECM and NM plants. This suggests that drought stress significantly limits the growth and photosynthetic performance of masson pine seedlings. Nevertheless, increased An/gs and proline contents in both NM and ECM prevented oxidative damage caused by drought stress. In addition, increased peroxidase (POD) activity is an essential defense mechanism of ECM seedling under drought stress. Compared with NM, ECM seedlings showed faster growth, higher RWC, and photosynthetic performance, and lower lipid peroxidation in cell membranes under drought stress, as indicated by higher POD activity and lower proline and malondialdehyde (MDA). Our experiment found that S. placidus inoculation can enhance the drought resistance of masson pine seedlings by increasing antioxidant enzyme activity, water use efficiency, and proline content, thereby enhancing growth under water-deficiency conditions. S. placidus can be used to cultivate high-quality seedlings and improve their survival in regions that experience seasonal droughts.

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