A histochemical study of sclerotia of Botrytis cinerea and Botrytis fabae

1984 ◽  
Vol 30 (2) ◽  
pp. 171-178 ◽  
Author(s):  
D. Backhouse ◽  
H. J. Willetts
Keyword(s):  
Botrytis Cinerea ◽  
Cell Walls ◽  
Histochemical Study ◽  
Botrytis Fabae ◽  
Nutrient Reserves

Histochemical techniques were used to determine the nature and distribution of nutrient reserves and other materials in sclerotia of two isolates of Botrytis cinerea and one isolate of B. fabae. All isolates showed similar staining reactions. Hyphal walls contained chitin and β-glucans, including β-1,3-glucans, while rind cell walls also contained a melanic pigment. Medullary hyphae were surrounded by a continuous matrix of β-glucans. The intracellular nutrient reserves found were protein, glycogen, polyphosphate, and lipid.

scholarly journals Genome-wide transcriptional profiling of Botrytis cinerea genes targeting plant cell walls during infections of different hosts

2014 ◽  
Vol 5 ◽  
Author(s):  
Barbara Blanco-Ulate ◽  
Abraham Morales-Cruz ◽  
Katherine C. H. Amrine ◽  
John M. Labavitch ◽  
Ann L. T. Powell ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Plant Cell ◽  
Cell Walls ◽  
Transcriptional Profiling ◽  
Plant Cell Walls ◽  
Genome Wide

scholarly journals Application of molecular methods for characterization of Botrytis fabae and Botrytis cinerea of faba bean

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 314-318
Author(s):  
N.M. Abou-Zeid ◽  
I.H. Dorriah ◽  
A.A. Marwa
Keyword(s):  
Botrytis Cinerea ◽  
Faba Bean ◽  
Morphological Characters ◽  
Molecular Methods ◽  
Pcr Analysis ◽  
Conidial Morphology ◽  
Rapd Pcr ◽  
Botrytis Fabae

Application of the RAPD methods allowed to clearly characterization of isolates of Botrytis fabae and isolates of B. cinerea. Results from RAPD-PCR analysis indicate different groups. Clusters were related with groups based on conidial morphology, morphological characters of the isolates of Botrytis spp.

scholarly journals Ultrastructural and Cytochemical Aspects of the Biological Control of Botrytis cinerea by Candida saitoana in Apple Fruit

1998 ◽  
Vol 88 (4) ◽  
pp. 282-291 ◽  
Author(s):  
Ahmed El-Ghaouth ◽  
Charles L. Wilson ◽  
Michael Wisniewski
Keyword(s):  
Host Cell ◽  
Botrytis Cinerea ◽  
Cell Walls ◽  
Close Contact ◽  
Defense Responses ◽  
Apple Fruit ◽  
Penicillium Expansum ◽  
Fungal Colonization ◽  
Biocontrol Activity ◽  
Apple Tissue

Biocontrol activity of Candida saitoana and its interaction with Botrytis cinerea in apple wounds were investigated. When cultured together, yeast attached to Botrytis sp. hyphal walls. In wounded apple tissue, C. saitoana restricted the proliferation of B. cinerea, multiplied, and suppressed disease caused by either B. cinerea or Penicillium expansum. In inoculated apple tissue without the yeast, fungal colonization caused an extensive degradation of host walls and altered cellulose labeling patterns. Hyphae in close proximity to the antagonistic yeast exhibited severe cytological injury, such as cell wall swelling and protoplasm degeneration. Colonization of the wound site by C. saitoana did not cause degradation of host cell walls. Host cell walls in close contact with C. saitoana cells and B. cinerea hyphae were well preserved and displayed an intense and regular cellulose labeling pattern. In addition to restricting fungal colonization, C. saitoana induced the formation of structural defense responses in apple tissue. The ability of C. saitoana to prevent the necrotrophic growth of the pathogen and stimulate structural defense responses may be the basis of its biocontrol activity.

Hitting the Wall: Plant Cell Walls During Botrytis cinerea Infections

2015 ◽  
pp. 361-386 ◽  
Author(s):  
Barbara Blanco-Ulate ◽  
John M. Labavitch ◽  
Estefania Vincenti ◽  
Ann L. T. Powell ◽  
Dario Cantu
Keyword(s):  
Botrytis Cinerea ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls

scholarly journals Physical, Anatomical, and Biochemical Composition of Skins Cell Walls from Two Grapevine Cultivars (Vitis vinifera) of Champagne Region Related to Their Susceptibility to Botrytis cinerea during Ripening

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 413
Author(s):  
Marie André ◽  
Soizic Lacampagne ◽  
Audrey Barsacq ◽  
Etienne Gontier ◽  
Melina Petrel ◽  
...  
Keyword(s):  
Cell Wall ◽  
Botrytis Cinerea ◽  
Cell Walls ◽  
Disease Incidence ◽  
Morphological Characteristics ◽  
Pinot Noir ◽  
Molecular Characteristics ◽  
Pectic Polysaccharides ◽  
Grape Skin ◽  
Skin Cell

This work investigated the structural, biochemical, and molecular characteristics of grape skin cell wall during ripening, related to susceptibility to Botrytis cinerea. The comparative study between the two main grape cultivars in Champagne region, Pinot noir and Chardonnay, quantified: (1) the maturity and physical profile of grape skin; (2) the morphological characteristics; (3) soluble pectic polysaccharides located in grape skin cell walls; and (4) the gene expression of the two main degrading enzymes (VvPME1 and VvPG1) and PME activity. During the maturation period, the grape skins of the two cultivars appear different in their structure and composition. Chardonnay is characterized by higher relative humidity (RH) and level of VvPG1 expression, lower disease incidence and penetrometry values, and thicker cell walls than Pinot noir skins. Thus, the cell wall composition is sufficiently different between grape varieties from the same area to allow their discrimination and could be used to better manage the harvest date.

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