Molecular evidence for a close phylogenetic relationship among box-shaped halophilic bacteria, Halobacterium vallismortis, and Halobacterium marismortui

1983 ◽  
Vol 29 (1) ◽  
pp. 52-59 ◽  
Author(s):  
Diarmuid E. Nicholson ◽  
George E. Fox
Keyword(s):  
Phylogenetic Relationship ◽  
New Genus ◽  
Sequence Data ◽  
Halophilic Bacteria ◽  
5S Rrna ◽  
Molecular Evidence ◽  
Rrna Sequence ◽  
Close Phylogenetic Relationship ◽  
Single Genus

The 5S rRNAs from Halobacterium vallismortis, Halobacterium marismortui (the Ginzburg strain), and two extremely halophilic isolates that exhibit an unusual "box-shaped" morphology were examined by ribonuclease T1 fingerprinting. The 5S rRNAs are found to be essentially identical and it is concluded that the four strains are in fact close phylogenetic relatives that should properly be assigned to a single genus. Comparison with previously available 5S rRNA sequence data from Halobacterium cutirubrum and Halococcus morrhuae indicates that the four strains examined here are quite distinct from both. It is further argued that this molecular evidence is not inconsistent with the previously proposed creation of a new genus, Haloarcula.

Reanalysis of 5S rRNA sequence data for the Vibrionaceae with the clustan program suite

1987 ◽  
Vol 15 (6) ◽  
pp. 329-335 ◽  
Author(s):  
Steven P. Nearhos ◽  
John A. Fuerst
Keyword(s):  
Sequence Data ◽  
5S Rrna ◽  
Rrna Sequence

Molecular Analysis of Leonurus Species in China Based on ITS and matK Sequences

2011 ◽  
Vol 39 (02) ◽  
pp. 411-422 ◽  
Author(s):  
Zhi-Ye Yang ◽  
Sheng-Li Pan ◽  
Ke-Ke Huo ◽  
Bing-Yi Wu ◽  
Zhi Chao
Keyword(s):  
Phylogenetic Relationship ◽  
Molecular Analysis ◽  
Maximum Parsimony ◽  
Sequence Data ◽  
Its Sequences ◽  
Interspecific Relationship ◽  
Neighbor Joining ◽  
Natural Group ◽  
Close Phylogenetic Relationship ◽  
Its Regions

The genus Leonurus has long been recognized as a natural group, but its interspecific relationship has not yet been studied in the light of sequence data. The ITS regions and matK sequences of all subgenera of Leonurus in China were amplified, sequenced and investigated. Phylogenies generated by maximum parsimony and neighbor-joining methods and division of the genus into two major clades. The phylogenetic results indicated that L. chaituroides has the very close phylogenetic relationship with Subg. Cardiochilium and supported the notion that L. macranthus acts as the bridge between Subg. cardiochilium and Subg. Leonurus. According to the analysis of information given by ITS and matK sequences, we suggest that ITS sequences would be more suitable to serve as markers for authentication of Herba Leonuri than matK does.

scholarly journals Isolates of ‘Candidatus Nostocoida limicola’ Blackall et al. 2000 should be described as three novel species of the genus Tetrasphaera, as Tetrasphaera jenkinsii sp. nov., Tetrasphaera vanveenii sp. nov. and Tetrasphaera veronensis sp. nov.

2006 ◽  
Vol 56 (10) ◽  
pp. 2279-2290 ◽  
Author(s):  
C. M. McKenzie ◽  
E. M. Seviour ◽  
P. Schumann ◽  
A. M. Maszenan ◽  
J.-R. Liu ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Data ◽  
23S Rrna ◽  
Phenotypic Characterization ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Hybridization Data ◽  
Intergenic Regions ◽  
Single Genus ◽  
Additional Support

Despite differences in their morphologies, comparative analyses of 16S rRNA gene sequences revealed high levels of similarity (>94 %) between strains of the filamentous bacterium ‘Candidatus Nostocoida limicola’ and the cocci Tetrasphaera australiensis and Tetrasphaera japonica and the rod Tetrasphaera elongata, all isolated from activated sludge. These sequence data and their chemotaxonomic characters, including cell wall, menaquinone and lipid compositions and fingerprints of their 16S–23S rRNA intergenic regions, support the proposition that these isolates should be combined into a single genus containing six species, in the family Intrasporangiaceae in the Actinobacteria. This suggestion receives additional support from DNA–DNA hybridization data and when partial sequences of the rpoC1 gene are compared between these strains. Even though few phenotypic characterization data were obtained for these slowly growing isolates, it is proposed, on the basis of the extensive chemotaxonomic and molecular evidence presented here, that ‘Candidatus N. limicola’ strains Ben 17, Ben 18, Ben 67, Ben 68 and Ben 74 all be placed into the species Tetrasphaera jenkinsii sp. nov. (type strain Ben 74T=DSM 17519T=NCIMB 14128T), ‘Candidatus N. limicola’ strain Ben 70 into Tetrasphaera vanveenii sp. nov. (type strain Ben 70T=DSM 17518T=NCIMB 14127T) and ‘Candidatus N. limicola’ strains Ver 1 and Ver 2 into Tetrasphaera veronensis sp. nov. (type strain Ver 1T=DSM 17520T=NCIMB 14129T).

scholarly journals Michaelmoelleria (Gesneriaceae), a new lithophilous dwelling genus and species with zigzag corolla tube from southern Vietnam

PhytoKeys ◽  
2020 ◽  
Vol 146 ◽  
pp. 89-107
Author(s):  
Fang Wen ◽  
Zi-Bing Xin ◽  
Long-Fei Fu ◽  
Shu Li ◽  
Lan-Ying Su ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
New Genus ◽  
Sequence Data ◽  
Its Region ◽  
Corolla Tube ◽  
Single Species ◽  
Molecular Evidence ◽  
Conservation Assessment ◽  
Dna Sequence Data ◽  
Southern Vietnam

Michaelmoelleria, a new genus from southern Vietnam is described with a single species, M. vietnamensis. The new genus is morphologically most similar to Deinostigma and Tribounia but it differs from the latter two by having four fertile stamens. Nuclear ribosomal internal transcribed spacer (ITS) region and plastid trnL-F intron spacer (trnL-F) DNA sequence data from the new genus and eighty-seven species representing 42 genera within tribe Didymocarpeae are used to resolve its generic placement. The molecular evidence reveals that it is most closely related to Cathayanthe rather than Deinostigma and Tribounia. The chromosome number is counted as 2n = 36 that further clarified its distinction comparing to the related genera within tribe Didymocarpeae. A global conservation assessment is also performed and classifies Michaelmoelleria vietnamensis as Critically Endangered (CR).

scholarly journals Yuxiensis granularis gen. et sp. nov., a Novel Quellkörper-Bearing Fungal Taxon Added to Scortechiniaceae and Inclusion of Parasympodiellaceae in Coronophorales Based on Phylogenetic Evidence

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1011
Author(s):  
Digvijayini Bundhun ◽  
Dhanushka N. Wanasinghe ◽  
Sajeewa S. N. Maharachchikumbura ◽  
Darbhe J. Bhat ◽  
Shi-Ke Huang ◽  
...  
Keyword(s):  
Posterior Probability ◽  
New Genus ◽  
Sequence Data ◽  
Bayesian Posterior Probability ◽  
The Novel ◽  
Incertae Sedis ◽  
Fungal Taxon ◽  
Close Phylogenetic Relationship ◽  
Relationship Of ◽  
Probability Analyses

An undetermined saprobic fungal taxon from Yunnan (China) is revealed as a new genus in Scortechiniaceae (Coronophorales). The novel taxon, Yuxiensis, is characterized by immersed to erumpent, semi-globose ascomata, which are not surrounded by any tomentum or conspicuous subiculum, a subcylindrical quellkörper in the centrum, clavate asci with long pedicels and allantoid hyaline ascospores with granular contents. Maximum likelihood and Bayesian posterior probability analyses based on LSU, ITS, tef1 and rpb2 sequence data depict a close phylogenetic relationship of the new genus to Pseudocatenomycopsis, hence, confirming its placement in Scortechiniaceae. Parasympodiellaceae, thus far belonging to Parasympodiellales, is transferred to Coronophorales based on multi-gene phylogenetic evidence. Additionally, the incertae sedis monotypic genus Arthrocristula is treated as a synonym of Parasympodiella, with Arthrocristula hyphenata recombined as Parasympodiella hyphenata comb. nov., as the type strain of Arthrocristula hyphenata clusters inside the Parasympodiellaceae clade along with other Parasympodiella taxa.

Nerve cells of Xenoturbella bocki (phylum uncertain) and Harrimania kupfferi (Enteropneusta) are positively immunoreactive to antibodies raised against echinoderm neuropeptides

2005 ◽  
Vol 85 (6) ◽  
pp. 1519-1524 ◽  
Author(s):  
Thomas Stach ◽  
Samuel Dupont ◽  
Olle Israelson ◽  
Geraldine Fauville ◽  
Hiroaki Nakano ◽  
...  
Keyword(s):  
Phylogenetic Relationship ◽  
Dna Sequence ◽  
Sequence Data ◽  
Nerve Cells ◽  
Phylogenetic Position ◽  
Dna Sequence Data ◽  
Close Phylogenetic Relationship ◽  
Positive Immunoreactivity ◽  
Phylogenetic Hypotheses ◽  
Relationship Of

The phylogenetic position of Xenoturbella spp. has been uncertain since their discovery in 1949. It has been recently suggested that they could be related to Ambulacraria within Deuterostomia. Ambulacraria is a taxon that has been suggested to consist of Hemichordata and Echinodermata. The hypothesis that X. bocki was related to Ambulacraria as well as the hypothesis of a monophyletic Ambulacraria is primarily based on the analysis of DNA sequence data. We tested both phylogenetic hypotheses using antibodies raised against SALMFamide 1 and 2 (S1, S2), neuropeptides isolated from echinoderms, on X. bocki and the enteropneust Harrimania kupfferi. Both species showed distinct positive immunoreactivity against S1 and S2. This finding supports the Ambulacraria-hypothesis and suggests a close phylogenetic relationship of X. bocki to Ambulacraria. In particular, the presence of immunoreactivity against S2 can be interpreted as a synapomorphy of Enteropneusta, Echinodermata, and Xenoturbella spp.

scholarly journals Ligninsphaeria jonesii gen. et. sp. nov., a remarkable bamboo inhabiting ascomycete

Phytotaxa ◽  
2016 ◽  
Vol 247 (2) ◽  
pp. 109 ◽  
Author(s):  
JIN-FENG ZHANG ◽  
JIAN-KUI LIU ◽  
KEVIN D. HYDE ◽  
YONG-XIANG LIU ◽  
ALI H. BAHKALI ◽  
...  
Keyword(s):  
New Genus ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Molecular Evidence ◽  
New Taxon ◽  
Monophyletic Clade ◽  
Incertae Sedis ◽  
Distinct Lineage

A new ascomycete species, inhabiting decaying bamboo culms, is introduced in this paper based on morphology and molecular evidence. Analyses of partial LSU sequence data place the taxon in a monophyletic clade within the order Pleosporales. Morphological characters of the taxon are also distinct from other members of Pleosporales. Ligninsphaeria jonesii gen. et sp. nov. is therefore introduced to accommodate the new taxon, which is characterized by its clypeate, scattered, deeply immersed ascomata, with a slit-like opening, long ostioles, cellular pseudoparaphyses, clavate asci containing broad-fusiform and 1-septate ascospores surrounded by a gelatinous cap at both ends. The phylogenetic analyses show that the new taxon is phylogenetically close but distinct from the families Amniculicolaceae and Testudinaceae, and forms a distinct clade from other taxa in Pleosporales. The new taxon is described and compared with similar taxa. A new genus Ligninsphaeria (Pleosporales, incertae sedis) is introduced to accommodate this distinct lineage.

scholarly journals Disjunct distribution of Uzelothripidae (Thysanoptera) new to Australia

Zootaxa ◽  
2009 ◽  
Vol 2207 (1) ◽  
pp. 67-68
Author(s):  
DESLEY J TREE
Keyword(s):  
Phylogenetic Relationship ◽  
Disjunct Distribution ◽  
The Third ◽  
Close Phylogenetic Relationship ◽  
The Family ◽  
Single Genus

The order Thysanoptera (thrips) is divided into two sub-orders, Terebrantia with eight families, and Tubulifera with just one family. Within Terebrantia, the family Uzelothripidae contains only a single genus and species, Uzelothrips scabrosus Hood. This is believed to have derived independently from an early offshoot of the thysanopteran sub-order Terebrantia (Mound & Marullo, 1996), and has no close phylogenetic relationship with any other thrips taxa (Mound & Morris 2007). The form of the wings, antennae, cephalic tentorium and female ovipositor are unlike those of any other Thysanoptera (Fig. 1). The forewings (when present) have no longitudinal veins, but have marginal cilia arising from sockets. The antennae are exceptionally long and slender, and the third segment has a unique circular sensorium ventrally near the apex. The cephalic tentorium is well developed, and the ovipositor is membranous and protusible with no developed valves (Mound et al. 1980, Moritz et al. 2001).

scholarly journals Construction of the mycoplasma evolutionary tree from 5S rRNA sequence data.

1985 ◽  
Vol 82 (4) ◽  
pp. 1160-1164 ◽  
Author(s):  
M. J. Rogers ◽  
J. Simmons ◽  
R. T. Walker ◽  
W. G. Weisburg ◽  
C. R. Woese ◽  
...  
Keyword(s):  
Sequence Data ◽  
Evolutionary Tree ◽  
5S Rrna ◽  
Rrna Sequence
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