An examination of fluorescence and pigment production by Rhizopus species on coconut agar medium

1982 ◽  
Vol 28 (12) ◽  
pp. 1399-1401
Author(s):  
D. P. Thompson ◽  
Broderick E. Eribo
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ultraviolet Light ◽  
Agar Medium ◽  
Yellow Pigment ◽  
Green Fluorescence ◽  
Incandescent Light ◽  
Orange Yellow ◽  
Layer Chromatography

On coconut agar medium, all species of Rhizopus examined produced an orange–yellow pigment when observed under incandescent light and a blue–green fluorescence under longwave ultraviolet light. However, results obtained from thin-layer chromatography suggested that the characteristics were due to the presence of secondary metabolites other than aflatoxin.

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

Direct Fluorescent Detection of Organothiophosphorus Pesticides and Some of Their Sulfur-containing Breakdown Products After Thin Layer Chromatography

1967 ◽  
Vol 50 (5) ◽  
pp. 1088-1098
Author(s):  
Mohamed Tawfik H Ragab
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Ultraviolet Light ◽  
Congo Red ◽  
Fluorescent Detection ◽  
Bromine Vapor ◽  
Layer Chromatography ◽  
Sulfur Containing ◽  
Dark Blue

Abstract A rapid, simple, convenient, and widely applicable method for the direct fluorescent detection of organothiophosphorus pesticides and some postulated breakdown products of these compounds is presented. The coinpounds were spotted on thin layer chromatographic sheets, developed in ethyl acetate :nhexane, and made visible by exposure to bromine vapor followed by spraying with ferric chloride and 2-(o-hydroxy phenyl) benzoxazole. Of the 47 compounds tested, 32 compounds produced fluorescent blue spots vinder longwave ultraviolet light; these consisted of 25 organothiophosphorus pesticides, 5 sulfur-containing breakdown products, and phosphoric and hypophosphorous acids. A superimposed Congo red spray destroyed the fluorescence and resulted in dark blue spots against a red backgrovind. The sensitivity of this method is in the range of 0.2 to 5.0 μg, depending on the specific compound.

Thin-Layer Chromatographic Detection and Identification of Methaqualone Metabolites in Urine

1975 ◽  
Vol 21 (1) ◽  
pp. 76-80 ◽  
Author(s):  
H Kenneth Sleeman ◽  
James A Cella ◽  
Johnnie L Harvey ◽  
Douglas J Beach
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ultraviolet Light ◽  
Silica Gel ◽  
Chloroform Extract ◽  
Detection And Identification ◽  
Positive Urine ◽  
Chromatographic Detection ◽  
Layer Chromatography ◽  
Urine Specimens

Abstract A procedure for detecting methaqualone and identifying methaqualone metabolites in urine by thin-layer chromatography is described and evaluated. Urine is hydrolyzed with HCl or NalO4, adjusted to pH 9.5, and extracted with chloroform. The chloroform extract is evaporated, reconstituted in methanol, applied to fluorescent silica-gel plates, and developed with ethyl acetate:methanol:ammonium hydroxide (28%) (85:10:5 by vol). Methaqualone use is detected by a pattern of four metabolites, which can be seen under ultraviolet light or are made visible by acidified iodoplatinate reagent. Synthetic methaqualone metabolites are used for identification and to compensate for procedural variables. More than 250 positive urine specimens were correctly identified by this method. Hydrolyzed natural and synthetic metabolites were identical by several criteria.

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN MAHONI (Swietenia mahagoni (L.) Jacq.) TERHADAP LARVA Aedes aegypti L.

2017 ◽  
Vol 4 (2) ◽  
pp. 23
Author(s):  
Tisa Rizkika Nur Amelia
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Thin Layer ◽  
Key Words ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
Botanical Insecticide ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Swietenia Mahagoni

<p>The aim of the research were to evaluate the efficacy of botanical insecticide of <em>S. mahagoni </em>leaves extracts against larvae of <em>Ae. aegypti,</em> based on concentration of the leaves <em>S. mahagoni </em>extract, and in additional to determine secondary metabolites compounds of <em>S. mahagoni </em>leaves extract. The extraction of <em>S. mahagoni </em>leaves was done by using ethanol solvents and than was analyzed by using Thin Layer Chromatography. The result indicated that ethanolic extract of <em>S. mahagoni </em>leaf contained alkaloid, tannin, saponin, terpenoid, and flavonoid compounds. The value of LC<sub>50 </sub>and LC<sub>90</sub> calculation showed that LC<sub>50</sub> of ethanolic extract over second and third instar larvae respectively were 488 ppm and 644 ppm. However the value of LC<sub>90</sub> of both instar larvae were 732 ppm and 797 ppm. Based on the above result, it can be concluded that ethanolic extract of <em>S. mahagoni </em>leaf was effective against larvae of <em>Ae .aegypti</em>.</p><p>Key words: <em>Ae. aegypti</em>, <em>S. mahagoni</em>, botanical insecticide</p>

TELAAH FITOKIMIA KULIT KACANG TANAH (Arachis hypogaea, L).

2021 ◽  
Vol 10 (1) ◽  
pp. 82-91
Author(s):  
Rizki Nisfi Ramdhini ◽  
Isna Mulyani ◽  
Syaikhul Aziz
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Waste Product ◽  
Phytochemical Screening ◽  
Medicinal Products ◽  
Processing Industry ◽  
Arachis Hypogaea L ◽  
Tlc Analysis ◽  
Layer Chromatography

Peanut peel are a waste product of the peanut processing industry with little commercial value. Some of studies have been conducted indicating peanut peel can be beneficial as a source for traditional medicinal products since it is also rich of antioxidants. The aim of this research was to identify the content of secondary metabolites on the peanut peel. The method used was maseration with 96% ethanol. Phytochemical screening and assaying were performed using thin layer chromatography (TLC) method. The results of TLC analysis showed that the secondary metabolites in peanut peel were positive for flavonoids, alkaloids, tannins and quinon. Keywords: Peanut peel, Phytochemical, Thin-Layer chromatography (TLC)

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